Date published: 2025-9-7
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α-actinin Antibody (H-2) - Western Blotting - Image 384018
α-actinin 抗体 (H-2) HRP: sc-17829 HRP. A-673 (A), L8 (B), K-562 (C), HeLa (D), A-10 (E) 和 SJRH30 (F) 全细胞裂解液中 α-actinin 表达的直接 western 印迹分析.
α-actinin 抗体 (H-2) HRP: sc-17829 HRP. A-673 (A), L8 (B), K-562 (C), HeLa (D), A-10 (E) 和 SJRH30 (F) 全细胞裂解液中 α-actinin 表达的直接 western 印迹分析.

α-actinin 抗体 (H-2): sc-17829

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说明书
  • alpha-actinin 抗体 H-2 是小鼠单克隆 IgG1 κ，α-actinin抗体, 在197篇文献中引用，规格为200 µg/ml
  • 免疫human物种的α-actinin-1的氨基酸593-892
  • alpha-actinin 抗体 (H-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 α-actinin isoforms
  • 抗alpha-actinin抗体（H-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取α-actinin (H-2): sc-17829的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 α-actinin 抗体 (H-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α-actinin 抗体 (H-2) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 α-actinin 抗体包括 α-actinin-1 和 α-actinin-4

α-肌动蛋白抗体（H-2）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的α-肌动蛋白蛋白 ）。α-肌动蛋白抗体（H-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。α-肌动蛋白在细胞骨架中起着至关重要的作用，它是一种捆绑蛋白，可将丝状肌动蛋白（F-肌动蛋白）交联，从而将肌动蛋白丝锚定到各种细胞内结构上。这种结构完整性对于维持细胞形状、实现细胞运动和促进肌肉收缩至关重要。值得注意的是，编码α-肌动蛋白-4的基因缺陷可导致局灶性节段性肾小球硬化症1（FSGS1），这是一种严重的肾脏疾病，其特征是进行性肾功能不全和潜在的肾衰竭。包括α-肌动蛋白在内的spectrin基因家族对于细胞骨架的正常组织至关重要，了解这些蛋白质的相互作用和功能对于阐明它们在健康和疾病中的作用至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α-actinin 抗体 (H-2) 参考文献:

  1. zyxin的一个α-肌动蛋白结合位点对亚细胞zyxin定域和α-肌动蛋白招募至关重要。  |  Reinhard, M., et al. 1999. J Biol Chem. 274: 13410-8. PMID: 10224105
  2. 富半胱氨酸蛋白中α-肌动蛋白结合位点的精细图谱。  |  Harper, BD., et al. 2000. Biochem J. 350 Pt 1: 269-74. PMID: 10926853
  3. 半球体成分与肌动蛋白家族成员的相互作用  |  Gonzalez, AM., et al. 2001. J Cell Sci. 114: 4197-206. PMID: 11739652
  4. α-肌动蛋白-长春新碱相互作用的结构动力学。  |  Bois, PR., et al. 2005. Mol Cell Biol. 25: 6112-22. PMID: 15988023
  5. 人中性粒细胞中的α-肌动蛋白和长链蛋白：黏合剂过程中的重组以及与肌动蛋白网络的关系。  |  Yürüker, B. and Niggli, V. 1992. J Cell Sci. 101 (Pt 2): 403-14. PMID: 1629252
  6. 依赖于α-肌动蛋白的细胞骨架锚定对 ICAM-5 介导的神经突起非常重要。  |  Nyman-Huttunen, H., et al. 2006. J Cell Sci. 119: 3057-66. PMID: 16820411
  7. α-肌动蛋白的结构和调节。  |  Sjöblom, B., et al. 2008. Cell Mol Life Sci. 65: 2688-701. PMID: 18488141
  8. α-肌动蛋白：一种在B细胞驱动的自身免疫中发挥重要作用的多学科蛋白。  |  Oikonomou, KG., et al. 2011. Autoimmun Rev. 10: 389-96. PMID: 21241830
  9. 人类肝细胞癌中人类α-肌动蛋白的表达。  |  Nishiyama, M., et al. 1990. Cancer Res. 50: 6291-4. PMID: 2169343
  10. 人类细胞骨架α-肌动蛋白基因的克隆和染色体定域揭示了与β-pectrin基因的联系。  |  Youssoufian, H., et al. 1990. Am J Hum Genet. 47: 62-72. PMID: 2349951
  11. CaMKII和α-肌动蛋白-2相互作用是树突棘增大的分子基础。  |  Curtis, AJ., et al. 2023. Elife. 12: PMID: 37489746
  12. α-肌动蛋白通过α-卡替宁与粘连蛋白/卡替宁细胞-细胞粘附复合体相互作用。  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

订购信息

产品名称产品编号规格价格数量收藏夹

α-actinin 抗体 (H-2)

sc-17829
200 µg/ml
RMB2377.00

α-actinin (H-2): m-IgG Fc BP-HRP 套装

sc-528328
200 µg Ab; 10 µg BP
RMB2662.00

α-actinin (H-2): m-IgGκ BP-HRP 套装

sc-520674
200 µg Ab, 40 µg BP
RMB2662.00

α-actinin (H-2): m-IgG1 BP-HRP 套装

sc-542868
200 µg Ab; 20 µg BP
RMB2662.00

α-actinin 抗体 (H-2) AC

sc-17829 AC
500 µg/ml, 25% agarose
RMB3129.00

α-actinin 抗体 (H-2) HRP

sc-17829 HRP
200 µg/ml
RMB2377.00

α-actinin 抗体 (H-2) FITC

sc-17829 FITC
200 µg/ml
RMB2482.00

α-actinin 抗体 (H-2) PE

sc-17829 PE
200 µg/ml
RMB2580.00

α-actinin 抗体 (H-2) Alexa Fluor® 488

sc-17829 AF488
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 546

sc-17829 AF546
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 594

sc-17829 AF594
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 647

sc-17829 AF647
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 680

sc-17829 AF680
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 790

sc-17829 AF790
200 µg/ml
RMB2685.00

α-actinin 抗体 (H-2) Alexa Fluor® 647の日本語SDSを送付お願いいたします。 ない場合は、日本の法規制に該当するか教えていただけますようお願いいたします。

Asked by: ハラレイコ
質問頂き、誠にありがとうございます。資料の申請、又は質問がございましたら、日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。 Thank you very much for your question. If you would like to apply for materials or have any questions, please contact your Japanese dealer and ask. Thank you for your cooperation.
Answered by: Jiajun Z
Date published: 2023-11-29

Does this antibody detect all 4 isoforms of alpha-actinin?

Asked by: MsMoonlight
Thank you for your inquiry. This antibody has a C-terminal epitope (within amino acids 593-892 ), so all isoforms present in the c-terminal would be possible to be picked up. Unfortunately the antibody has not been epitope mapped, so the exact epitope is not known.
Answered by: Tech Service
Date published: 2019-02-22

Can I freeze sc-17829 antibody for long time storage?

Asked by: Carlaat
Thank you for your question. No, freezing the antibody will negatively impact its viability. Our antibodies are contained in buffer with PBS, gelatin and sodium azide. When frozen, they precipitate out of the solution and may denature. The antibodies are best stored at 4 °C and if handled properly will retain their reactivity for several years.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

From the description of applications I guess this antibody should work for IP, but I can't find any review comment on the result of IP. Do you have any test result picture or publication using this antibody for IP? Thanks!

Asked by: Yali
Thank you for your question. This antibody is currently recommended and warrantied for IP, however we are still gathering data to show on our website. Check back for updates! There is also at least one product citation using this antibody successfully for IP/WB. Please refer to PMID: 16966331 to see their data with this antibody.
Answered by: Technical Support
Date published: 2022-06-15

Can α-actinin Antibody (H-2): sc-17829 be used for detection of NOS1 of pig origin?

Asked by: jenni
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from greatI’ve tested this antibody for IF on iPSCs-derived cardiomyocytes. The signal is very clear.
Date published: 2024-07-23
Rated 4 out of 5 by from Good for WBGood antibody, worked well diluted 1:1000 for western blot
Date published: 2022-02-04
Rated 4 out of 5 by from Great featuresI receveid the trial sample of this antibody 2 weeks ago and i tested it on Melanoma cell lines A375 and SK-Mel-5 in Western Blot at 1:1000. With odissey scanner i obtained well defined band around 95kDa.
Date published: 2021-12-14
Rated 5 out of 5 by from Good for both IF and WBThis antibody worked in IF and WB both in human melanoma cell line MDA-MB-435S. It showed differential expression among the cell lines and the expression was in line with the mass spectrometry results.
Date published: 2021-06-07
Rated 5 out of 5 by from Great resultsI got a sample of a-actinin antibody to use as a loading control, and it has been very successful. I started with the recommended dilution and have had to dilute it much more to avoid oversaturating our ECL detection reagent. We will purchase the full size soon.
Date published: 2020-02-04
Rated 4 out of 5 by from Good antibodyThe antibody is pretty good. Depending on the length of exposure an extra band (or bands) can be seen, which makes it less than perfect
Date published: 2019-12-14
Rated 5 out of 5 by from Detects chicken α-actinin for Western BlottingUsed a 1:1000 dilution, was able to detect alpha-actinin within chicken DF1 cells by Western Blotting with excellent signal.
Date published: 2019-04-02
Rated 5 out of 5 by from great signal for mouse muscle samplesUsed to detect a-actinin in mouse muscle samples, signal looks great.
Date published: 2019-01-23
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α-actinin抗体 (H-2) is rated 4.9 out of 5 by 31.
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