Date published: 2025-9-6
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α-Actin Antibody (alpha-SM1) - Western Blotting - Image 360929
α-Actin 抗体 (α-SM1): sc-130617. A-10 (A) 和 L6 (B) 全细胞裂解液中 α-Actin 表达的 Western 印迹分析.
α-Actin 抗体 (α-SM1): sc-130617. A-10 (A) 和 L6 (B) 全细胞裂解液中 α-Actin 表达的 Western 印迹分析.

α-Actin 抗体 (alpha-SM1): sc-130617

4.4(5)
寫評論提問

说明书
  • α-Actin抗体(alpha-SM1)是小鼠单克隆IgG2a κ, 在69篇文献中引用，规格为200 µg/ml
  • 针对human起源的smooth muscle α-Actin的N-terminal十肽
  • 推荐用于 mouse, rat 和 human 来源的smooth muscle α-Actin WB, IP, IF, IHC(P) 和 ELISA检测; 不会与 actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)产生交联反应
  • 另提供琼脂糖偶联抗体，可用于IP实验
  • 看 α-Actin (1A4): sc-32251，可查看有标记的 α-Actin 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG2a BP-HRP 是α-Actin 抗体 (alpha-SM1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与α-Actin 抗体 (alpha-SM1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Actin 抗体包括 α-Actin, β-Actin, γ-Actin, Muscle Actin 和 Smooth Muscle Actin

所有真核细胞都会表达肌动蛋白，这种蛋白通常占细胞总蛋白的50%。肌动蛋白丝能够形成稳定和不稳定的结构，并且是微绒毛和肌肉细胞收缩装置的关键组成部分。较低等的真核生物（如酵母）只有一个肌动蛋白基因，而较高等的真核生物则具有由一个基因家族编码的多种异构体。哺乳动物组织中至少存在六种肌动蛋白，它们分为三类。α-肌动蛋白的表达仅限于各种类型的肌肉组织，而β-肌动蛋白和γ-肌动蛋白则是其他组织中肌动蛋白丝的主要成分。小GTP酶家族的成员调节肌动蛋白细胞骨架的组织结构。其中，Rho控制肌动蛋白应力纤维和粘着斑的组装，Rac调节肌动蛋白丝在质膜上的积聚，而Cdc42则刺激丝状伪足的形成。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

α-Actin 抗体 (alpha-SM1)

sc-130617
200 µg/ml
RMB2377.00

α-Actin (alpha-SM1): m-IgG2a BP-HRP 套装

sc-546020
200 µg Ab; 10 µg BP
RMB2662.00

α-Actin 抗体 (alpha-SM1) AC

sc-130617 AC
500 µg/ml, 25% agarose
RMB3129.00

I look for ZAP-70 antibodies for flowcytometry, detector PE, reactivity human, for 100 samples, this test for academic research

Asked by: Mallasi
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Service
Date published: 2022-03-28

Can this α-Actin Antibody (alpha-SM1)：sc-130617 be used to stain α-smooth muscle actin in vascular endothelial cell？

Asked by: ny1164
Thank you for your question. We have tested this antibody in vascular endothelial cells so we can not confirm reactivity in this cell type.
Answered by: Technical Support
Date published: 2019-01-25

Can α-Actin (alpha-SM1): sc-130617 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, α-Actin (alpha-SM1): sc-130617 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-15
  • y_2025, m_9, d_6, h_5CST
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Rated 4 out of 5 by from Activation of Adiponectin Receptor Regulates ProprWe used the anti-SMA antibody to determine expression of smooth muscle actin in the atortic root cross section by immunofluorescent staining, and obtained a good results. We presented the results as Figure 6D in our published paper.
Date published: 2017-08-17
Rated 5 out of 5 by from Great reactivityA great performing antibody in blotting and staining procedures. It gives off a clear and specific signal and has great reactivity. This antibody has been used to publish more than 25 times.
Date published: 2017-02-07
Rated 5 out of 5 by from It worked well in WBIt worked well in WB, IHC, IF.
Date published: 2015-10-13
Rated 4 out of 5 by from Western blot data with human aortic SMCsWestern blot data with human aortic SMCs. -SCBT QC
Date published: 2013-05-01
Rated 4 out of 5 by from Decent bands in Western with rat pulmonaryDecent bands in Western with rat pulmonary fibroblasts. -SCBT QC
Date published: 2010-03-26
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α-Actin 抗体 (alpha-SM1) is rated 4.4 out of 5 by 5.
  • y_2025, m_9, d_6, h_5
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