Date published: 2025-9-6
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α-Actin Antibody (1A4) - Western Blotting - Image 396211
α-Actin 抗体 (1A4) Alexa Fluor® 647: sc-32251 AF647. C2C12 (A), A-10 (B), BC3H1 (C), Sol8 (D) 和 L6 (E) 全细胞裂解液中 α-Actin 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
α-Actin 抗体 (1A4) Alexa Fluor® 647: sc-32251 AF647. C2C12 (A), A-10 (B), BC3H1 (C), Sol8 (D) 和 L6 (E) 全细胞裂解液中 α-Actin 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

α-Actin 抗体 (1A4): sc-32251

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说明书
  • alpha-Actin 抗体 1A4 是小鼠单克隆 IgG2a κ，α-Actin抗体, 在414篇文献中引用，规格为200 µg/ml
  • 针对human起源的α-Actin的N-terminal十肽
  • alpha-Actin 抗体 (1A4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 smooth muscle α-Actin; 不会与 actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)产生交联反应
  • 抗alpha-Actin抗体（1A4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取α-Actin (1A4): sc-32251的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 α-Actin 抗体 (1A4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α-Actin 抗体 (1A4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Actin抗体，包括α-Actin, β-Actin, γ-Actin, Muscle Actin和Smooth Muscle Actin

α-肌动蛋白抗体（1A4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的α-肌动蛋白蛋白。抗α-肌动蛋白抗体（1A4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌动蛋白作为细胞骨架的关键组成部分，在细胞结构和功能中发挥着至关重要的作用，为细胞提供机械支撑和形状。α-肌动蛋白在细胞质中形成动态丝状结构，对细胞的各种过程至关重要，包括运动、分裂和细胞内运输。α-肌动蛋白的快速聚合和解聚使细胞能够根据环境线索调整形状和运动，因此这种蛋白质对于伤口愈合和免疫反应等过程至关重要。α-肌动蛋白对肌肉细胞的收缩装置有显著贡献，使肌肉能够收缩和放松。小GTP酶家族成员通过协调肌动蛋白丝的组装和拆卸来严格控制α-肌动蛋白的动态，确保细胞正常功能和刺激反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α-Actin 抗体 (1A4) 参考文献:

  1. 骨骼肌α-肌动蛋白基因（ACTA1）突变引起的肌肉疾病。  |  Sparrow, JC., et al. 2003. Neuromuscul Disord. 13: 519-31. PMID: 12921789
  2. 心脏α-肌动蛋白过度表达疗法在显性ACTA1疾病中的应用。  |  Ravenscroft, G., et al. 2013. Hum Mol Genet. 22: 3987-97. PMID: 23736297
  3. 血管平滑肌细胞分化中的核α-肌动蛋白  |  Kwartler, CS., et al. 2023. Res Sq.. PMID: 36909460
  4. 平滑肌α-肌动蛋白错义变异可通过调节平滑肌细胞中的细胞内胆固醇来促进动脉粥样硬化。  |  Kaw, K., et al. 2023. Eur Heart J. 44: 2713-2726. PMID: 37377039
  5. 核平滑肌α-肌动蛋白参与血管平滑肌细胞分化。  |  Kwartler, CS., et al. 2023. Nat Cardiovasc Res. 2: 937-955. PMID: 38919852
  6. 微管相关蛋白在控制微管组装中的作用  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
  7. Rho, rac和cdc42 GTP酶调节与肌动蛋白应力纤维, 薄片和丝状体相关的多分子缔合作用。  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
  8. 关于 F-肌动蛋白建模的讨论。  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
  9. 肌动蛋白细胞骨架设定细胞运动的节奏  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
  10. 真核生物蛋白质的起源和进化。  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
  11. 弹性纤维是人类胎盘干绒毛基质的重要组成部分，也是血管周围收缩鞘的组成部分。  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
  12. 激光共聚焦显微镜揭示的人类毛囊中的肌动蛋白束。  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

订购信息

产品名称产品编号规格价格数量收藏夹

α-Actin 抗体 (1A4)

sc-32251
200 µg/ml
RMB2377.00

α-Actin (1A4): m-IgG Fc BP-HRP 套装

sc-528446
200 µg Ab; 10 µg BP
RMB2662.00

α-Actin (1A4): m-IgGκ BP-HRP 套装

sc-520826
200 µg Ab, 40 µg BP
RMB2662.00

α-Actin (1A4): m-IgG2a BP-HRP 套装

sc-547102
200 µg Ab; 10 µg BP
RMB2662.00

α-Actin 抗体 (1A4) AC

sc-32251 AC
500 µg/ml, 25% agarose
RMB3129.00

α-Actin 抗体 (1A4) HRP

sc-32251 HRP
200 µg/ml
RMB2377.00

α-Actin 抗体 (1A4) FITC

sc-32251 FITC
200 µg/ml
RMB2482.00

α-Actin 抗体 (1A4) PE

sc-32251 PE
200 µg/ml
RMB2580.00

α-Actin 抗体 (1A4) Alexa Fluor® 488

sc-32251 AF488
200 µg/ml
RMB2685.00

α-Actin 抗体 (1A4) Alexa Fluor® 546

sc-32251 AF546
200 µg/ml
RMB2685.00

α-Actin 抗体 (1A4) Alexa Fluor® 594

sc-32251 AF594
200 µg/ml
RMB2685.00

α-Actin 抗体 (1A4) Alexa Fluor® 647

sc-32251 AF647
200 µg/ml
RMB2685.00

α-Actin 抗体 (1A4) Alexa Fluor® 680

sc-32251 AF680
200 µg/ml
RMB2685.00

α-Actin 抗体 (1A4) Alexa Fluor® 790

sc-32251 AF790
200 µg/ml
RMB2685.00

I wonder if this antibody can be used to identify muscle cells？

Asked by: Anonymous
Thank you for your question. alpha-Actin Antibody (1A4) is recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric).
Answered by: Technical Service SH
Date published: 2023-03-17

I failed in ELISA with this antibody. Briefly, I coated alpha-actin on Ni plate, applied this antibody HRP, substrate TMB. No color.

Asked by: LucyLu
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-07

Quel est le tampon de démasquage et la concentration utilisé pour un marquage sur coupe paraffine ?

Asked by: acertain
Merci pour votre question. Sur coupes en paraffine, nous recommandons d’effectuer un démasquage de l’antigène en tampon citrate. Vous trouverez plus de précisions dans notre page protocoles dans le lien suivant : https://www.scbt.com/scbt/resources/protocols Thank you for your question. In IHC on paraffin embedded tissues sections, we recommend to perform antigen retrieval in citrate buffer. You will find additional information in our protocols in the following link : https://www.scbt.com/scbt/resources/protocols
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

If I want to perform a Western blot using the α-Actin Antibody (1A4) on human samples, what secondary reagent should I use for detection?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for triple staining even if my other primary antibodies were also raised in mice?

Asked by: Trav11
When looking to perform triple staining with multiple mouse primary antibodies, we recommend using our directly conjugated mouse monoclonal primary antibodies. The α-Actin Antibody (1A4): sc-32251 is currently available conjugated to HRP (sc-32251 HRP), phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647).
Answered by: Tech Service 8
Date published: 2016-12-16

I would like to know whether this antibody is usable to evaluate the expression pattern of alfa-actin protein in adult zebrafish especially skeletal muscles. If not, could you please tell us the other useful antibodies?

Asked by: Yosuke
Thank you for your question. α-Actin Antibody (1A4): sc-32251 is recommended for detection of smooth muscle α-Actin, rather than skeletal muscle actin isoforms. This antibody has not yet been tested in zebrafish, and it is unknown if it would work for that species. As a recommended alternative, we would suggest our mouse monoclonal, β-Actin Antibody (C4): sc-47778. This antibody is recommended for detection of β-Actin; may cross-react with all six known isoforms of Actin (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform). This antibody has also been cited in the literature used in zebrafish in the following articles on NCBI's PubMed: PMID: 25075304, 22139372.
Answered by: Technical Support
Date published: 2016-12-02
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Rated 5 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-19
Rated 5 out of 5 by from α-SMA specific antibodyThe antibody was successfully used in IF and flow cytometry during mouse embryonic stem cell differentiation.
Date published: 2024-02-27
Rated 5 out of 5 by from alpha-SMAThis antibody was used successfully for Western blot (1:2000) with clear background.
Date published: 2022-09-26
Rated 4 out of 5 by from Good AntibodyThe antibody works perfectly for immuofluorescence on human cardiac fibroblast activated with TGFBeta
Date published: 2022-03-04
Rated 5 out of 5 by from spécifique et croise avec des espèces de renteJ'utilise cet anticorps depuis quelques années car il croise avec l'espèce bovine et donne des résultats spécifiques
Date published: 2022-01-07
Rated 5 out of 5 by from very clean backgroundWB recommends the use of 1:1000 dilution, antibody specific binding and strong band appearance. So far, no other experiments have been done.
Date published: 2017-11-01
Rated 1 out of 5 by from Not well work in WBToo many unspecific WB band detected in human liver cancer BEL-7404 lysates. dilution ratio is 1:100
Date published: 2017-10-11
Rated 4 out of 5 by from works good on IF of tissue samplesWe used this product for the detection of a-SMA in the mouse fibrotic liver frozen sections.
Date published: 2017-10-04
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α-Actin 抗体 (1A4) is rated 4.7 out of 5 by 31.
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