Date published: 2025-9-6

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α-Actin 抗体 (5C5): sc-58670

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说明书
  • α-Actin抗体(5C5)是小鼠单克隆IgM κ, 在34篇文献中引用,规格为200 µg/ml
  • 推荐用于 mouse, rat, human, rabbit, bovine 和 Xenopus 来源的α-skeletal and α-cardiac Actin WB, IP, IF 和 IHC(P)检测; 不建议用于检测α-Actin from smooth muscle tissue
  • 看 α-Actin (1A4): sc-32251,可查看有标记的 α-Actin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取α-Actin (5C5): sc-58670免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 α-Actin 抗体 (5C5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与α-Actin 抗体 (5C5) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

α-肌动蛋白抗体(5C5)是一种小鼠单克隆IgM抗体,可通过应用诸如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠、人、兔和牛样品中的α-肌动蛋白。抗-α-肌动蛋白抗体(5C5)以非结合形式提供,可满足各种实验需求。肌动蛋白是一种高度保守的蛋白质,在细胞结构和运动中起着关键作用,在真核细胞中占细胞总蛋白的50%。α-肌动蛋白单克隆抗体(5C5)对于研究微绒毛的形成和肌肉细胞中的收缩装置至关重要,有助于肌肉收缩和细胞运动。肌动蛋白有多种同源形式,其中α-肌动蛋白在肌肉组织中表达特别明显,而β-肌动蛋白和γ-肌动蛋白在非肌肉细胞中占主导地位。肌动蛋白细胞骨架的组织结构由小GTP酶家族成员(包括Rho、Rac和Cdc42)进行复杂调控,它们分别负责协调肌动蛋白应力纤维的组装、肌动蛋白丝在质膜上的积聚以及纤毛的形成。了解α-肌动蛋白的动态及其相互作用对于阐明肌肉功能和各种疾病的潜在机制至关重要,因此α-肌动蛋白(5C5)抗体成为该领域研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

α-Actin 抗体 (5C5)

sc-58670
200 µg/ml
RMB2377.00

α-Actin (5C5): m-IgGκ BP-HRP 套装

sc-550733
200 µg Ab; 40 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with α-Actin (5C5): sc-58670 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from The signal is presentI used the Ab on epithelial renal cells by IF. The signal on my cells is present, even if not as good as in the datasheet.
Date published: 2022-02-08
Rated 5 out of 5 by from 特异性很好,没有杂带很抱歉这么晚才来反馈,因为学校刚刚放假,所以才有时间做实验,a-actin这款抗体让人非常满意,准备购买一支大包装,继续使用(图片添加一直失败)
Date published: 2018-01-29
Rated 5 out of 5 by from NiceWB: Good data with mouse heart tissue, the expected results are obtained
Date published: 2017-05-14
Rated 5 out of 5 by from a very great antibodyUse this antibody for many times, It works nicely on rat heart tissue extract western blot.
Date published: 2017-04-26
Rated 5 out of 5 by from ExcellentIt is well for Western Blot when we got it. 1/1000 dilution!This product use effect is very good, our company will continue to buy
Date published: 2017-01-10
Rated 5 out of 5 by from very goodIt is well for Western Blot when we got it. 1/1000 dilution!This product use effect is very good, our company will continue to buy
Date published: 2017-01-04
Rated 5 out of 5 by from Good staining with frozen rat heart sectionsGood staining with frozen rat heart sections, incubated overnight. -SCBT Publication Review
Date published: 2013-04-19
Rated 5 out of 5 by from Good loading control for Western BlotGood loading control for Western Blot. -SCBT Publication Review
Date published: 2013-02-27
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α-Actin 抗体 (5C5) is rated 4.8 out of 5 by 8.
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