Date published: 2025-9-6

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α-Actin 抗体 (0.N.5): sc-58669

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说明书
  • α-Actin抗体(0.N.5)是小鼠单克隆IgG2a κ, 在33篇文献中引用,规格为200 µg/ml
  • 针对human起源的α-Actin的N-terminal十肽
  • 推荐用于 mouse, rat 和 human 来源的smooth muscle α-Actin WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: bovine; 不会与 actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)产生交联反应
  • 看 α-Actin (1A4): sc-32251,可查看有标记的 α-Actin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 α-Actin 抗体 (0.N.5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α-Actin 抗体 (0.N.5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

α-肌动蛋白抗体(0.N.5)是一种小鼠单克隆IgG2a抗体,可通过多种应用检测小鼠、大鼠和人类样本中的α-肌动蛋白,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)。α-肌动蛋白在肌肉收缩和 细胞结构中起着关键作用,因为抗-α-肌动蛋白抗体(0.N.5)可识别细胞骨架的关键组成部分,为细胞提供机械支撑和形状。α-肌动蛋白主要存在于肌肉组织中,α-肌动蛋白单克隆抗体(0.N.5)可检测肌肉功能所必需的收缩装置,也存在于非肌肉细胞中,有助于各种细胞过程,如运动和分裂。肌动蛋白有多种同源物,包括α-、β-和γ-肌动蛋白,它们在不同的细胞类型中发挥不同的功能,其中α-肌动蛋白与肌肉细胞有特殊关联。了解α-肌动蛋白的动态变化及其与其他蛋白质(如小GTP酶家族成员)的相互作用,对于阐明细胞运动和稳定机制至关重要,因此α-肌动蛋白(0.N.5)抗体成为研究肌肉生物学和细胞骨架动态的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

α-Actin 抗体 (0.N.5)

sc-58669
200 µg/ml
RMB2377.00

α-Actin (0.N.5): m-IgG Fc BP-HRP 套装

sc-539093
200 µg Ab; 10 µg BP
RMB2662.00

α-Actin (0.N.5): m-IgG2a BP-HRP 套装

sc-546669
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended starting dilution for Western blot for sc-58669: α-Actin (0.N.5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Worked well for immunofluorescenceUsed the antibody at 1:500 dilution for immunofluorescence in FFPE rabbit skin with antigen retrieval performed in a pressure cooker in a sodium citrate pH 6.0 buffer. Successfully stained myofibroblasts in scar tissue, as well as smooth muscle cells in blood vessels.
Date published: 2023-01-14
Rated 5 out of 5 by from immunofluorescenceAntibody was successfully used for immunofluorescence experiments in adipose-derived stem cells at a concentration of 1:2000
Date published: 2019-03-06
Rated 5 out of 5 by from sc-58669 very nice atibody for SM α-ActinI can get very clear bands at 43KDa with 1:10000 dilution of 1st Ab and the same dilution of 2nd Ab. Excellent !
Date published: 2017-08-14
Rated 4 out of 5 by from Good IF with porcine heartGood IF with porcine heart, 1:100 overnight at 4 C. -SCBT Publication Review
Date published: 2011-04-22
Rated 4 out of 5 by from Worked well in paraffinWorked well in paraffin-embedded mouse tissue. 1:25 dilution. -SCBT Publication Review
Date published: 2011-01-11
Rated 5 out of 5 by from Good IF staining inGood IF staining in -20 C methanol fixed PAC1 cells. 1:100 dilution. -SCBT Publication Review
Date published: 2010-12-22
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α-Actin 抗体 (0.N.5) is rated 4.7 out of 5 by 6.
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