Date published: 2025-9-7

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Akt1 抗体 (G-5): sc-55523

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说明书
  • Akt1抗体(G-5)是小鼠单克隆IgG1 κ, 在50篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Akt1的氨基酸345-480
  • 抗-Akt1 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Akt1
  • 抗Akt1抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Akt1 (G-5): sc-55523免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Akt1 Antibody (G-5) for WB applications. 的首选辅助检测试剂,这些试剂现与Akt1 Antibody (G-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Akt1 抗体 (G-5) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 等应用检测小鼠、大鼠和人样本中的 Akt1。抗 Akt1 抗体 (G-5) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Akt1 又称蛋白激酶 B (PKB),在调节细胞生长、存活和新陈代谢的细胞信号通路中起着至关重要的作用。当生长因子与各自的受体结合,导致磷脂酰肌醇 3- 激酶(PI3K)招募并随后在 Thr 308 和 Ser 473 处磷酸化 Akt1 时,Akt1 就开始被激活。磷酸肌醇依赖性激酶-1(PDK1)的 Thr 308 磷酸化对 Akt1 的激酶活性至关重要,而由 mTOR 复合物 2(mTORC2)介导的 Ser 473 磷酸化可增强酶的功能并稳定活性构象。这种双重磷酸化使 Akt1 能够传播生存和生长信号,并促进与糖原合酶激酶-3β(GSK-3β)和叉头盒蛋白(FOXO)等特定下游底物的相互作用,这些底物在新陈代谢和细胞凋亡中发挥着关键作用。对 Akt1 激活进行精确的空间和时间调控至关重要,因为不适当或长时间的激活会破坏细胞稳态,导致癌症和胰岛素抵抗等病理状况。Akt1 与包括哺乳动物雷帕霉素靶标(mTOR)在内的各种下游靶标的相互作用是蛋白质合成和细胞生长调控不可或缺的一部分,这凸显了 Akt1 作为重要治疗靶标在癌症生物学和代谢紊乱中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Akt1 抗体 (G-5)

sc-55523
200 µg/ml
$316.00

Akt1 (G-5): m-IgG Fc BP-HRP 套装

sc-526862
200 µg Ab; 10 µg BP
$354.00

Akt1 (G-5): m-IgG1 BP-HRP 套装

sc-532235
200 µg Ab; 20 µg BP
$354.00

Akt1 抗体 (G-5) AC

sc-55523 AC
500 µg/ml, 25% agarose
$416.00

Akt1 抗体 (G-5) HRP

sc-55523 HRP
200 µg/ml
$316.00

Akt1 抗体 (G-5) FITC

sc-55523 FITC
200 µg/ml
$330.00

Akt1 抗体 (G-5) PE

sc-55523 PE
200 µg/ml
$343.00

Akt1 抗体 (G-5) Alexa Fluor® 488

sc-55523 AF488
200 µg/ml
$357.00

Akt1 抗体 (G-5) Alexa Fluor® 546

sc-55523 AF546
200 µg/ml
$357.00

Akt1 抗体 (G-5) Alexa Fluor® 594

sc-55523 AF594
200 µg/ml
$357.00

Akt1 抗体 (G-5) Alexa Fluor® 647

sc-55523 AF647
200 µg/ml
$357.00

Akt1 抗体 (G-5) Alexa Fluor® 680

sc-55523 AF680
200 µg/ml
$357.00

Akt1 抗体 (G-5) Alexa Fluor® 790

sc-55523 AF790
200 µg/ml
$357.00

Does this recognize AKt-2 and Akt-3?

Asked by: Sundance
Thank you for your question. sc-55523Akt1 (G-5) does not detect Akt2 and Akt 3. https://www.uniprot.org/uniprotkb/P31749/entry
Answered by: BlakeJ
Date published: 2025-02-25

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-55523: Akt1 (G-5) mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from 很棒的抗體這支抗體專一性高,背景低訊號清晰穩定性佳,適用多種實驗表現一致性好,重複性高信號集中清楚!!!!!!
Date published: 2025-07-18
Rated 5 out of 5 by from good antibodyI like to work with santa cruz antibodies. They work great!
Date published: 2019-11-05
Rated 4 out of 5 by from Great antibodiesI used this antibodies in dilution 1:1000 in tissues samples. It works great.
Date published: 2018-06-26
Rated 4 out of 5 by from Great productI got free sample from company. The antibodies work in WB in dilution 1:1000.
Date published: 2018-06-10
Rated 5 out of 5 by from Great antibodyVery nice results from hippocampus tissue by WB analysis, in 1:500 dilution.
Date published: 2018-05-24
Rated 5 out of 5 by from GreatThis antibody is great. After the trial, we order this antibody again.
Date published: 2018-02-14
Rated 5 out of 5 by from Un ottimo anticorpoHo utilizzato il capione di prova lo scorso dicembre 2017. Ho diluito l'anticorpo 1:500 in PBS+BSA. Il segnale che ho ottenuto è stato pulito, solo un aspecifico sopra i 90 KDa. Consigliato!
Date published: 2018-01-19
Rated 3 out of 5 by from works moderatelyThis antibody gives works but the blot is a little dirty. AKT1 (B-1) works way better.
Date published: 2017-11-17
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Akt1 抗体 (G-5) is rated 4.6 out of 5 by 15.
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