Date published: 2025-9-7

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Akt1 抗体 (F-8): sc-271149

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说明书
  • Akt1抗体(F-8)是小鼠单克隆IgG1 κ, 在14篇文献中引用,规格为200 µg/ml
  • 与氨基酸序列C-terminus(h)对应的多肽序列(Akt1的human起源)
  • 推荐用于 mouse, rat 和 human 来源的Akt1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 Akt1 (B-1): sc-5298,可查看有标记的 Akt1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Akt1 Antibody (F-8) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与Akt1 Antibody (F-8) 打包提供(请参阅下面的订购信息)。

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関連項目

丝氨酸/苏氨酸激酶Akt家族包含几个成员,包括Akt1(也被命名为PKB或RacPK)、Akt2(也被命名为PKBβ或RacPK-βb)和Akt3(也被命名为PKBγ或胸腺病毒原癌基因3),它们与蛋白激酶A和C家族具有序列同源性,并由c-Akt原癌基因编码。Akt家族的所有成员都具有血小板同源结构域。Akt1和Akt2通过血小板源性生长因子(PDGF)的刺激被激活。这种激活依赖于PDGFR-β的酪氨酸残基740和751,这些残基与磷脂酰肌醇3-激酶(PI 3-激酶)复合物的亚基结合。胰岛素或胰岛素生长因子-1(IGF-1)对Akt1的激活导致Thr 308和Ser 473的磷酸化。这两个残基的磷酸化对于产生高水平的Akt1活性至关重要。在体内,Thr 308的磷酸化不依赖于Ser 473的磷酸化。因此,在胰岛素/IGF-1刺激的细胞中,Akt蛋白通过上游激酶被磷酸化和激活。PI激酶抑制剂渥曼青霉素会抑制Akt1和Akt2的激活,这表明该蛋白是PI激酶下游的信号分子。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Akt1 抗体 (F-8)

sc-271149
200 µg/ml
RMB2377.00

Akt1 (F-8): m-IgG Fc BP-HRP 套装

sc-540213
200 µg Ab; 10 µg BP
RMB2662.00

Akt1 (F-8): m-IgG1 BP-HRP 套装

sc-541975
200 µg Ab; 20 µg BP
RMB2662.00

Akt1 (F-8) 中和勝肽

sc-271149 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-271149 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Great productI used this antibodies in dilution 1:1000 in tissues samples. It works great.
Date published: 2018-06-26
Rated 5 out of 5 by from Positive immunoperoxidase staining of formalinPositive immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear and cytoplasmic staining of glandular cells. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from 55 kda signal via western blot analysis of55 kda signal via western blot analysis of Akt2 expression in non-transfected 293T, mouse Akt2 transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from WB publication using human vascular endothelialWB publication using human vascular endothelial cell lines, EA.hy 926 samples -SCBT Publication Review
Date published: 2015-03-10
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Akt1 抗体 (F-8) is rated 4.8 out of 5 by 4.
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