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Akt1/2/3 Antibody (BDI111) - Western Blotting - Image 17507
Akt1/2/3 抗体 (BDI111): sc-56878. A549 (A), MCF7 (B), HeLa (C), MIA PaCa-2 (D), ZR-75-1 (E) 和 BT-20 (F) 全细胞裂解液中 Akt1/2/3 表达的 Western 印迹分析.
Akt1/2/3 Antibody (BDI111) - Western Blotting - Image 17507
Akt1/2/3 抗体 (BDI111): sc-56878. A549 (A), MCF7 (B), HeLa (C), MIA PaCa-2 (D), ZR-75-1 (E) 和 BT-20 (F) 全细胞裂解液中 Akt1/2/3 表达的 Western 印迹分析.
Akt1/2/3 抗体 (BDI111): sc-56878. A549 (A), MCF7 (B), HeLa (C), MIA PaCa-2 (D), ZR-75-1 (E) 和 BT-20 (F) 全细胞裂解液中 Akt1/2/3 表达的 Western 印迹分析.

Akt1/2/3 抗体 (BDI111): sc-56878

4.3(7)
寫評論提問

说明书
  • Akt1/2/3 抗体 BDI111 是小鼠单克隆 IgG1, 在60篇文献中引用，提供 50 µg/0.5 ml
  • 与human来源的合成Akt1肽发生反应
  • 推荐用于 human 来源的Akt1 when it is dephosphorylated at Ser 473, Akt2 when it is dephosphorylated at Ser 474 and Akt3 when it is dephosphorylated at Ser 472 WB 和 IP检测
  • 看 Akt1 (B-1): sc-5298，可查看有标记的 Akt1/2/3 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成Akt1/2/3 抗体 (BDI111)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案
研究信息

関連項目

此外 Akt 抗体包括 Akt1, Akt2 和 Akt3

丝氨酸/苏氨酸激酶Akt家族包含几个成员，包括Akt1（也被命名为PKB或RacPK）、Akt2（也被命名为PKBβ或RacPK-βb）和Akt3（也被命名为PKBγ或胸腺病毒原癌基因3），它们与蛋白激酶A和C家族具有序列同源性，并由c-Akt原癌基因编码。Akt家族的所有成员都具有血小板同源结构域。Akt1和Akt2通过血小板源性生长因子（PDGF）的刺激被激活。这种激活依赖于PDGFR-β的酪氨酸残基740和751，这些残基与磷脂酰肌醇3-激酶（PI 3-激酶）复合物的亚基结合。胰岛素或胰岛素生长因子-1（IGF-1）对Akt1的激活导致Thr 308和Ser 473的磷酸化。这两个残基的磷酸化对于产生高水平的Akt1活性至关重要。在体内，Thr 308的磷酸化不依赖于Ser 473的磷酸化。因此，在胰岛素/IGF-1刺激的细胞中，Akt蛋白通过上游激酶被磷酸化和激活。PI激酶抑制剂渥曼青霉素会抑制Akt1和Akt2的激活，这表明该蛋白是PI激酶下游的信号分子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Akt1/2/3 抗体 (BDI111) 参考文献:

  1. 索拉非尼下调ERK/Akt和STAT3存活通路并诱导人神经母细胞瘤细胞系凋亡  |  Chai, H., et al. 2010. Int J Clin Exp Pathol. 3: 408-15. PMID: 20490331
  2. 非典型β1-肾上腺素能受体诱导人类尿道上皮增生。  |  Winder, M., et al. 2015. Auton Autacoid Pharmacol. 35: 32-40. PMID: 26913580
  3. 黄酮类衍生物 Morin-7-sulphate sodium 对小鼠黑色素瘤细胞的抗癌作用。  |  Li, HW., et al. 2016. Biomed Pharmacother. 84: 909-916. PMID: 27764752
  4. 白细胞介素6缺乏对细菌脂多糖诱导的小鼠心肌信号转导通路激活作用  |  Tarasiuk, E., et al. 2020. Adv Med Sci. 65: 386-393. PMID: 32693349
  5. Akt在获得性西妥昔单抗耐药头颈部鳞状细胞癌中的作用：一种新型联合疗法的体外研究。  |  Zaryouh, H., et al. 2021. Front Oncol. 11: 697967. PMID: 34568028
  6. CDK4 抑制剂 Fascaplysin 与表皮生长因子受体抑制剂阿法替尼联合治疗非小细胞肺癌的协同细胞毒性。  |  Plangger, A., et al. 2022. Invest New Drugs. 40: 215-223. PMID: 34596822
  7. 脂肪细胞和脂因子促进子宫平滑肌瘤的友好微环境。  |  Afrin, S., et al. 2023. Nutrients. 15: PMID: 36771423
  8. H2S以G6PD依赖的方式调节心脏β-肾上腺素能受体下游的氧化还原信号。  |  Chhabra, A., et al. 2023. Cell Signal. 107: 110664. PMID: 37004833
  9. AKT1和AKT2在生育、发情周期和子宫内膜腺体发育中具有冗余作用。  |  Adam, P., et al. 2023. Reproduction. 165: 605-616. PMID: 37053038
  10. AKT1通过直接磷酸化已糖激酶促进肿瘤发生和转移。  |  Yu, Y., et al. 2024. J Cell Biochem. 125: e30613. PMID: 38860522

订购信息

产品名称产品编号规格价格数量收藏夹

Akt1/2/3 抗体 (BDI111)

sc-56878
50 µg/0.5 ml
RMB2377.00

I am using Akt1/2/3 (BDI111): sc-56878 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great for WBI bought this antibody a few years ago and the result is good and clear.
Date published: 2024-03-20
Rated 4 out of 5 by from Great productThe antibodies works good. I used this antibody 1:1000 on Western blot
Date published: 2018-06-29
Rated 5 out of 5 by from Akt1/2/3 AntibodyWorked concentration at 1:1000 dilution in ARPE-19 cells with strong signal and low backgroud
Date published: 2018-01-03
Rated 4 out of 5 by from Western blot publication using cell lines HepG2Western blot publication using cell lines HepG2, SNU-387, PLC/PRF5 and HCCLM3. -SCBT Publication Review
Date published: 2015-03-10
Rated 4 out of 5 by from Positive western blot analysis of Akt1/2/3Positive western blot analysis of Akt1/2/3 expression in A549, MCF7, HeLa, MIA PaCa-2, ZR-75-1 and BT-20 whole cell lysates. -SCBT QC
Date published: 2015-02-28
Rated 4 out of 5 by from Publication for western blotting applicationPublication for western blotting application using HA22T/VGH and SK-Hep-1 samples. -SCBT Publication Review
Date published: 2015-02-18
Rated 4 out of 5 by from Publication using SKPublication using SK-Hep-1 and HA22T/VGH samples in western blotting. -SCBT Publication Review
Date published: 2015-01-08
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Akt1/2/3 抗体 (BDI111) is rated 4.3 out of 5 by 7.
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