Date published: 2025-9-7
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ADAR1 Antibody (D-8) - Western Blotting - Image 369808
ADAR1 抗体 (D-8): sc-271854. Ramos 全细胞裂解液 (A), HeLa (B) 和 K-562 (C) 核提取物中 ADAR1 表达的 Western 印迹分析. 所用检测试剂: m-IgGλ BP-HRP (Cruz Marker): sc-516132-CM.
ADAR1 抗体 (D-8): sc-271854. Ramos 全细胞裂解液 (A), HeLa (B) 和 K-562 (C) 核提取物中 ADAR1 表达的 Western 印迹分析. 所用检测试剂: m-IgGλ BP-HRP (Cruz Marker): sc-516132-CM.

ADAR1 抗体 (D-8): sc-271854

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寫評論提問

说明书
  • ADAR1抗体(D-8)是小鼠单克隆IgG1 λ ADAR1抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1051-1226位于human物种的ADAR1的C-terminus
  • ADAR1 抗体 (D-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 all ADAR1 isoforms
  • 抗ADAR1抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ADAR1 (D-8): sc-271854的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 ADAR1 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与ADAR1 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品，请参阅我们的 小鼠 IgG 结合蛋白完整列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 ADAR 抗体 包括 ADAR1, ADAR2和ADAR3

ADAR1抗体（D-8）是一种IgG1 λ小鼠单克隆ADAR1抗体（也称为DSRAD抗体、腺苷脱氨酶RNA特异性抗体、双链RNA特异性腺苷脱氨酶抗体、136 KDa双链RNA结合蛋白抗体、干扰素可诱导蛋白4抗体、干扰素诱导蛋白4抗体、DRADA抗体、IFI-4抗体、G1P1抗体、IFI4抗体、P136抗体、作用于RNA 1-A的腺苷脱氨酶抗体或DsRNA腺苷脱氨酶抗体），可通过WB、IP、IF、IHC(P)和ELISA检测所有人源ADAR1异构体。ADAR1抗体（D-8）既有非偶联型抗ADAR1抗体形式，也有多种偶联型抗ADAR1抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。RNA特异性腺苷脱氨酶（ADAR1、DSH、IFI4、p136、DRADA、DSRAD、K88dsRBP）通过使结构化或双链RNA中的腺苷脱氨为肌苷来破坏双链RNA的稳定性，从而介导RNA编辑。ADAR1由干扰素反应启动子表达，其N端具有Z-DNA/Z-RNA结合域。ADAR1与SUMO-1在核仁下区域共定位，该区域与纤维中心、致密纤维组分和颗粒组分不同。核ADAR1的定位受ADAR1中部的核仁定位信号（NoLS）和靠近N端的核输出信号（NES）的输出活性的影响。ADAR1通过与NF110、NF90和NF45相互作用上调核因子90（NF90）介导的基因表达。ADAR1结合小干扰RNA（siRNA），在ADAR1无效突变的纯合子小鼠成纤维细胞中，siRNA的基因沉默效果比在野生型细胞中显著更有效。ADAR1可能限制siRNA在哺乳动物细胞中的功效。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ADAR1 抗体 (D-8) 参考文献:

  1. 人类 ADAR1 可防止内源性 RNA 触发翻译关闭  |  Chung, H., et al. 2018. Cell. 172: 811-824.e14. PMID: 29395325
  2. 肿瘤中 ADAR1 的缺失可克服免疫检查点阻断剂的抗药性。  |  Ishizuka, JJ., et al. 2019. Nature. 565: 43-48. PMID: 30559380
  3. RNA编辑酶ADAR1在人类疾病中的作用。  |  Song, B., et al. 2022. Wiley Interdiscip Rev RNA. 13: e1665. PMID: 34105255
  4. ADAR1 限制 ZBP1 介导的免疫反应和 PAN 细胞凋亡，从而促进肿瘤发生。  |  Karki, R., et al. 2021. Cell Rep. 37: 109858. PMID: 34686350
  5. ADAR1及其在癌症发展和治疗中的意义。  |  Baker, AR. and Slack, FJ. 2022. Trends Genet. 38: 821-830. PMID: 35459560
  6. ADAR1掩盖了ZBP1驱动的坏死性细胞凋亡在癌症免疫治疗方面的前景。  |  Zhang, T., et al. 2022. Nature. 606: 594-602. PMID: 35614224
  7. ADAR1突变导致ZBP1依赖性免疫病理。  |  Hubbard, NW., et al. 2022. Nature. 607: 769-775. PMID: 35859177
  8. 先天免疫、细胞死亡和疾病中的ADAR1和ZBP1。  |  Karki, R. and Kanneganti, TD. 2023. Trends Immunol. 44: 201-216. PMID: 36710220
  9. ADAR1介导的SCD1 RNA编辑可导致胃癌的耐药性和自我更新。  |  Wong, TL., et al. 2023. Nat Commun. 14: 2861. PMID: 37208334
  10. ADAR1通过miR-21/A20信号传导，保护肺部巨噬细胞免受脓毒症引起的热解和肺部损伤。  |  Zhao, X., et al. 2024. Int J Biol Sci. 20: 464-485. PMID: 38169584

订购信息

产品名称产品编号规格价格数量收藏夹

ADAR1 抗体 (D-8)

sc-271854
200 µg/ml
RMB2377.00

ADAR1 (D-8): m-IgGλ BP-HRP 套装

sc-522078
200 µg Ab, 40 µg BP
RMB2662.00

ADAR1 抗体 (D-8) AC

sc-271854 AC
500 µg/ml, 25% agarose
RMB3129.00

ADAR1 抗体 (D-8) HRP

sc-271854 HRP
200 µg/ml
RMB2377.00

ADAR1 抗体 (D-8) FITC

sc-271854 FITC
200 µg/ml
RMB2482.00

ADAR1 抗体 (D-8) PE

sc-271854 PE
200 µg/ml
RMB2580.00

ADAR1 抗体 (D-8) Alexa Fluor® 488

sc-271854 AF488
200 µg/ml
RMB2685.00

ADAR1 抗体 (D-8) Alexa Fluor® 546

sc-271854 AF546
200 µg/ml
RMB2685.00

ADAR1 抗体 (D-8) Alexa Fluor® 594

sc-271854 AF594
200 µg/ml
RMB2685.00

ADAR1 抗体 (D-8) Alexa Fluor® 647

sc-271854 AF647
200 µg/ml
RMB2685.00

ADAR1 抗体 (D-8) Alexa Fluor® 680

sc-271854 AF680
200 µg/ml
RMB2685.00

ADAR1 抗体 (D-8) Alexa Fluor® 790

sc-271854 AF790
200 µg/ml
RMB2685.00

Can ADAR1 (D-8): sc-271854 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ADAR1 (D-8): sc-271854 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ADAR1 Western blot（HEK293细胞）我是用来跑HEK293细胞的蛋白样品，Western blot的结果，特异性条带很好，没什么非特异性的条带的产生。
Date published: 2018-04-19
Rated 5 out of 5 by from 抗体不错抗体很好用，申请后，邮寄很及时，并且该抗体在1:1000的稀释比例下，进行western blot实验，条带清晰可见！
Date published: 2017-12-08
Rated 5 out of 5 by from Nice antibody for WBI diluted this antibody at 1:200 for western blot. A strong band at the predicted size of ADAR1 was detected.
Date published: 2017-11-21
Rated 4 out of 5 by from works wellworks well for western (1:1000) in bone marrow derived macrophages.
Date published: 2017-10-19
Rated 1 out of 5 by from Did not work well for meA small sample I asked for free earlier seemed to work well. However, with the D-8 I ordered, I could not detect adar1. It also have a strong off target.
Date published: 2017-08-06
Rated 5 out of 5 by from Great Antibody for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-23
Rated 4 out of 5 by from good antibody for IFworks well for IF (1:100) in U2OS cells. not tested in other applications yet.
Date published: 2016-12-14
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of ADAR1 expression in NTERA-cl.D1, U-2 OS and Ramos whole cell lysates and HeLa and K-562 nuclear extracts. -SCBT QC
Date published: 2015-05-10
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ADAR1 抗体 (D-8) is rated 4.5 out of 5 by 11.
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