Date published: 2025-9-7
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ADAR1 Antibody (15.8.6) - Western Blotting - Image 393708
ADAR1 抗体 (15. 8. 6) Alexa Fluor® 488: sc-73408 AF488. NTERA-2 cl. D1 (A), Ramos (B), PC-3 (C), SK-BR-3 (D) 和 MOLT-4 (E) 全细胞裂解液中 ADAR1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
ADAR1 抗体 (15. 8. 6) Alexa Fluor® 488: sc-73408 AF488. NTERA-2 cl. D1 (A), Ramos (B), PC-3 (C), SK-BR-3 (D) 和 MOLT-4 (E) 全细胞裂解液中 ADAR1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

ADAR1 抗体 (15.8.6): sc-73408

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寫評論提問

说明书
  • ADAR1抗体(15.8.6)是小鼠单克隆IgG1 κ, 在89篇文献中引用，规格为200 µg/ml
  • 针对氨基酸440-826的抗性，对应ADAR1的中间区域，human起源
  • 抗-ADAR1 抗体 (15.8.6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 native and recombinant ADAR1; 不会与 other members of ADAR family产生交联反应
  • 抗ADAR1抗体（15.8.6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取ADAR1 (15.8.6): sc-73408的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 ADAR1 抗体 (15.8.6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ADAR1 抗体 (15.8.6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ADAR 抗体 包括 ADAR1, ADAR2和ADAR3

ADAR1抗体（15.8.6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的ADAR1。抗ADAR1抗体（15.8.6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。RNA特异性腺苷脱氨酶（ADAR1）在RNA编辑中起着至关重要的作用，它可将双链RNA中的腺苷转化为肌苷，这对调节基因表达和维持RNA稳定性至关重要。这种翻译后修饰会影响mRNA的编码潜力和非编码RNA的功能，从而影响各种细胞过程和对环境刺激的响应。ADAR1由干扰素反应启动子表达，其N端具有Z-DNA/Z-RNA结合结构域，对于结构化RNA相互作用至关重要。ADAR1与SUMO-1共定位在核仁外的特定区域，其核定位受核仁定位信号和核出口信号调控。ADAR1能够结合短干扰RNA（siRNA），这表明其在哺乳动物细胞中调节基因沉默效果方面发挥作用，突出了其在RNA生物学和治疗应用中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ADAR1 抗体 (15.8.6) 参考文献:

  1. 胚胎红细胞生成需要 RNA 编辑脱氨酶 ADAR1 基因。  |  Wang, Q., et al. 2000. Science. 290: 1765-8. PMID: 11099415
  2. RNA 编辑活动与 lnRNP 颗粒中的剪接因子缔合作用：核前 mRNA 处理机制  |  Raitskin, O., et al. 2001. Proc Natl Acad Sci U S A. 98: 6571-6. PMID: 11381114
  3. 人类 RNA 编辑酶 ADAR1 的核胞质分布受双链 RNA 结合结构域, 富亮氨酸导出信号和假定二聚结构域的调节。  |  Strehblow, A., et al. 2002. Mol Biol Cell. 13: 3822-35. PMID: 12429827
  4. ADAR1 在活细胞核内对蛋白质翻译的诱导不依赖于 RNA 编辑。  |  Herbert, A., et al. 2002. Mol Cell. 10: 1235-46. PMID: 12453429
  5. 作用于 RNA 的腺苷脱氨酶的 RNA 编辑活性需要二聚化。  |  Cho, DS., et al. 2003. J Biol Chem. 278: 17093-102. PMID: 12618436
  6. ADAR1 同工酶的亚细胞分布由三个核鉴别信号和一个调控基序协同决定。  |  Nie, Y., et al. 2004. J Biol Chem. 279: 13249-55. PMID: 14711814
  7. ADAR1 RNA脱氨酶限制了短干扰RNA在哺乳动物细胞中的功效。  |  Yang, W., et al. 2005. J Biol Chem. 280: 3946-53. PMID: 15556947
  8. 干扰素诱导的 RNA 腺苷脱氨酶 ADAR1 在病原体感染和小鼠胚胎发育过程中的表达涉及组织选择性启动子利用和替代剪接。  |  George, CX., et al. 2005. J Biol Chem. 280: 15020-8. PMID: 15677478
  9. 爪蟾卵母细胞中 RNA 编辑酶 ADAR1 的染色体贮存。  |  Sallacz, NB. and Jantsch, MF. 2005. Mol Biol Cell. 16: 3377-86. PMID: 15843431
  10. RNA编辑酶ADAR1的Zbeta结构域的晶体结构揭示了Z结构域之间不同的保守表面。  |  Athanasiadis, A., et al. 2005. J Mol Biol. 351: 496-507. PMID: 16023667
  11. ADAR1 通过双链 RNA 与 NF90 相互作用，并独立于 RNA 编辑调控 NF90 介导的基因表达。  |  Nie, Y., et al. 2005. Mol Cell Biol. 25: 6956-63. PMID: 16055709
  12. SUMO-1 修饰会改变 ADAR1 的编辑活性。  |  Desterro, JM., et al. 2005. Mol Biol Cell. 16: 5115-26. PMID: 16120648
  13. Z-RNA 在体外对 ADAR1 编辑活性的调节。  |  Koeris, M., et al. 2005. Nucleic Acids Res. 33: 5362-70. PMID: 16177183

订购信息

产品名称产品编号规格价格数量收藏夹

ADAR1 抗体 (15.8.6)

sc-73408
200 µg/ml
RMB2377.00

ADAR1 (15.8.6): m-IgG Fc BP-HRP 套装

sc-527004
200 µg Ab; 10 µg BP
RMB2662.00

ADAR1 (15.8.6): m-IgG1 BP-HRP 套装

sc-532377
200 µg Ab; 20 µg BP
RMB2662.00

ADAR1 抗体 (15.8.6) AC

sc-73408 AC
500 µg/ml, 25% agarose
RMB3129.00

ADAR1 抗体 (15.8.6) HRP

sc-73408 HRP
200 µg/ml
RMB2377.00

ADAR1 抗体 (15.8.6) FITC

sc-73408 FITC
200 µg/ml
RMB2482.00

ADAR1 抗体 (15.8.6) PE

sc-73408 PE
200 µg/ml
RMB2580.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 488

sc-73408 AF488
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 546

sc-73408 AF546
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 594

sc-73408 AF594
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 647

sc-73408 AF647
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 680

sc-73408 AF680
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) Alexa Fluor® 790

sc-73408 AF790
200 µg/ml
RMB2685.00

ADAR1 抗体 (15.8.6) B

sc-73408 B
200 µg/ml
RMB2407.00

 Can I use the ADAR1 antibody (15.8.6): sc-73408 for whole mount staining on mouse esophageal tissue? PFA is used for fixation.

Asked by: Anonymous
感谢您的提问，抗-ADAR1 抗体 (15.8.6) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的ADAR1。可以用于PFA固定的小鼠样本进行免疫荧光实验。谢谢。 Thank you for your question. Anti-ADAR1 Antibody (15.8.6) is recommended for detection of native and recombinant ADAR1 of mouse, rat and human origin by WB, IP, IF and IHC(P). It can be used for immunofluorescence in PFA fixed sample of mouse. Thank you.
Answered by: Jiajun Z
Date published: 2023-04-18

I wonder if I can order some of your positive expressed samples (such as Ramos, PC-3, MOLT-4 for Adar1 WB?

Asked by: hzheng
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

Would this antibody detect both the p150 and p110 variants of ADAR1?

Asked by: CrisR
Thank you for your question. The antibody can detect both variants, but what is detected depends on what is present in the samples that are being tested. The expected molecular weight using this antibody is ~136kDa. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2019-05-15

Can the ADAR1 Antibody (15.8.6): sc-73408 mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: AbPolly
Yes. We recommend using a directly conjugated monoclonal antibody. Please contact our Special Orders department for availability of direct conjugates of ADAR1 Antibody (15.8.6): sc-73408. Otherwise, see ADAR1 (D-8): sc-271854 for ADAR1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647
Answered by: Technical Support 12
Date published: 2017-01-13

What is the difference between sc-sc-73408 and sc-73408 B?

Asked by: AbPolly
Thank you for your inquiry. The product sc-73408 B is our ADAR1 antibody directly conjugated to biotin, great for use in applications such as IHC when doing signal amplification.
Answered by: Technical Service 11
Date published: 2017-01-11

What is the recommended secondary for ADAR1 Antibody (15.8.6): sc-73408 in Western blot?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2020-01-01
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Rated 5 out of 5 by from Work very well for human, but not good for rodentsWork very well for human, but not good for rodents
Date published: 2022-01-05
Rated 4 out of 5 by from Detects IFN-inducible bands >100 kD in WesternUsed in Western with blocking in either milk or BSA. Detects several prominent bands above 100 kDa in human cells lines. Immunoreactivity higher after IFN treatment or virus infection.
Date published: 2020-10-01
Rated 5 out of 5 by from Works well for IFWorks well in IF on human fibroblast cells both with MeOH and PFA fixation.
Date published: 2018-07-26
Rated 5 out of 5 by from Works well for endogenous proteinUsed to detect endogenous protein in fibroblasts of human origin. Worked at dilution of 1:100 and in methanol-fixed cells. Nice subnuclear staining.
Date published: 2018-04-04
Rated 5 out of 5 by from great featuresVery good western blot result for test mouse brain samples.
Date published: 2018-01-08
Rated 5 out of 5 by from Great Features!A month ago, I used HEK293 to do an antibody test.I found that is excellent ,I did not see other strips beside the different isoforms of adar1.so,I have confidence in santa.
Date published: 2017-07-18
Rated 5 out of 5 by from 这个抗体很赞ADAR1的抗体很好，特异性很强，我们实验室一直在用，用了大概三年了。未来希望一直可以用，可以一直合作
Date published: 2017-06-14
Rated 3 out of 5 by from 免疫组化做不出买了两支这样的抗体了，做人源细胞实验没问题，条带清楚，结果好，但是大鼠标本跑western blot条带模糊，做免疫组化完全做不出
Date published: 2017-04-27
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ADAR1 抗体 (15.8.6) is rated 4.8 out of 5 by 17.
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