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Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 Antibody (8E4) - Western Blotting - Image 350228
AChRα9 (8E4): sc-293282. 人重组 AChRα9 融合蛋白的 Western 印迹分析.
Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 Antibody (8E4) - Western Blotting - Image 350228
AChRα9 (8E4): sc-293282. 人重组 AChRα9 融合蛋白的 Western 印迹分析.
AChRα9 (8E4): sc-293282. 人重组 AChRα9 融合蛋白的 Western 印迹分析.

Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 (8E4): sc-293282

5.0(1)
寫評論提問

说明书
  • Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 8E4 是小鼠单克隆 IgG2a κ，Nicotinic Acetylcholine Receptor alpha 9/CHRNA9抗体, 在4篇文献中引用，规格为100 µg/ml
  • 免疫human物种的AChRα9的氨基酸139-221
  • 推荐用于 mouse, rat 和 human 来源的AChRα9 WB, IP 和 ELISA检测
  • 目前，我们还没有完成Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 (8E4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
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说明书与实验方案

関連項目

此外 AChR 抗体 包括 AChR α, AChR α 1, AChR α 2, AChR α 3, AChR α 4, AChR α 5, AChR α 6, AChR α 7, AChR α 8和AChR α 9

AChRα9抗体（8E4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的AChRα9蛋白。抗AChRα9抗体（8E4）可作为非结合型同种型抗体使用。AChRα9 (8E4) 抗体可识别一种在听觉系统中发挥关键作用的蛋白质，尤其是在耳蜗毛细胞中，AChRα9对于调节胆碱能橄榄耳蜗纤维与外毛细胞（OHCs）之间的传出突触传递至关重要。AChRα9是耳蜗毛细胞中唯一的烟碱型乙酰胆碱受体，主要表达于成年大鼠耳蜗外毛细胞基底区和内毛细胞顶端区。AChRα9通道可促进钙离子流入，这对调节听觉信号和保护毛细胞免受损伤至关重要。了解AChRα9的特定相互作用和功能有助于深入了解听觉机制和听觉障碍的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 (8E4) 参考文献:

  1. α9烟碱乙酰胆碱受体亚基在耳蜗传出神经的发育和功能中的作用  |  Vetter, DE., et al. 1999. Neuron. 23: 93-103. PMID: 10402196
  2. 小鸡的前庭毛细胞表达烟碱乙酰胆碱受体亚基α9。  |  Lustig, LR., et al. 1999. J Vestib Res. 9: 359-67. PMID: 10544374
  3. α9烟碱乙酰胆碱受体的高钙渗透性和钙阻滞。  |  Katz, E., et al. 2000. Hear Res. 141: 117-28. PMID: 10713500
  4. 人类烟碱型乙酰胆碱受体亚基α（α）9（CHRNA9）基因的染色体定位和特征。  |  Lustig, LR. and Peng, H. 2002. Cytogenet Genome Res. 98: 154-9. PMID: 12697997
  5. 大鼠II型前庭毛细胞中烟碱型乙酰胆碱受体亚基α9的表达。  |  Kong, WJ., et al. 2006. Acta Pharmacol Sin. 27: 1509-14. PMID: 17049129
  6. 反射修饰测听揭示了橄榄球耳蜗神经传递的双重作用。  |  Allen, PD. and Luebke, AE. 2017. Front Cell Neurosci. 11: 361. PMID: 29213229
  7. α9：一种在大鼠耳蜗毛细胞中表达的具有新药理特性的乙酰胆碱受体。  |  Elgoyhen, AB., et al. 1994. Cell. 79: 705-15. PMID: 7954834
  8. 通过单细胞RT-PCR揭示了听觉毛细胞中α9 n-ACh受体亚基的细胞特异性表达。  |  Glowatzki, E., et al. 1995. Proc Biol Sci. 262: 141-7. PMID: 8524909
  9. 大鼠耳蜗和前庭内耳中α9乙酰胆碱受体mRNA的发育表达。  |  Luo, L., et al. 1998. J Comp Neurol. 393: 320-31. PMID: 9548553
  10. 新生儿和成年耳蜗毛细胞中α9烟碱乙酰胆碱受体亚基的差异表达  |  Simmons, DD. and Morley, BJ. 1998. Brain Res Mol Brain Res. 56: 287-92. PMID: 9602155
  11. α9 尼古丁乙酰胆碱受体与 A 型γ-氨基丁酸, 甘氨酸和 3 型血清素受体具有相同的药理特性。  |  Rothlin, CV., et al. 1999. Mol Pharmacol. 55: 248-54. PMID: 9927615

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产品名称产品编号规格价格数量收藏夹

Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 (8E4)

sc-293282
100 µg/ml
RMB2377.00

I saw two clear bands in the WB picture showed on your website. And the isotype control is igG2a Kappa. My question is that the mAb is the full length of heavy chain and a light chain, or purified IgG2a kappa chain. Thank you.

Asked by: yu long
Thank you for your question. The Western blot data you are referring to on our website is not representative of the endogenous molecular weight for AChRα9. That is Western blot data in a human recombinant fusion protein. This is a complete IgG2a antibody. The light chain type is kappa.
Answered by: Technical Support
Date published: 2019-07-13

For Western Blot, is it recommended to use denatured or non-denatured conditions with AChRα9 (8E4): sc-293282 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Can be used for immunofluorescenceAntibody has been used to detect AChRα9 in mouse cochlea by immunofluorescence in published research. -SCBT QC
Date published: 2023-09-14
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Nicotinic Acetylcholine Receptor alpha 9/CHRNA9 抗体 (8E4) is rated 5.0 out of 5 by 1.
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