Date published: 2025-9-8

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AChE 抗体 (A-11): sc-373901

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说明书
  • AChE 抗体 A-11 是小鼠单克隆 IgG2a κ,AChE抗体, 在15篇文献中引用,规格为200 µg/ml
  • 免疫human物种的AChE的氨基酸481-614
  • AChE 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 AChE
  • 抗AChE抗体(A-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AChE (A-11): sc-373901免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是AChE Antibody (A-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与AChE Antibody (A-11) 打包提供(请参阅下面的订购信息)。

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関連項目

AChE抗体(A-11)是一种IgG2a κ小鼠单克隆AChE抗体(也称为ACEE抗体、ARACHE抗体、N-ACHE抗体、水解酶抗体、羧酸酯酶家族成员抗体、Yt血型抗体、YT抗体或乙酰胆碱酯酶(YT血型)抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的AChE蛋白。AChE抗体(A-11)既可以是非偶联的抗AChE抗体形式,也可以是多种偶联形式的抗AChE抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。乙酰胆碱酯酶(AChE)在突触连接处水解乙酰胆碱。选择性mRNA剪接产生三种形式的AChE。T型,也称为不对称型,是可溶的,存在于突触中。H型也称为球型,存在于细胞膜的外表面。R型的功能尚不清楚。AChE球型亚基通过GPI锚定在细胞膜上,不对称亚基则通过胶原尾锚定在基底膜成分上。AChE的催化亚基是由二硫键连接的同源二聚体组成的寡聚体。阿尔茨海默病患者大脑中胆碱能和非胆碱能神经元的AChE丢失。然而,AChE活性在淀粉样斑块周围增加,这可能是由于涉及L型电压依赖性钙通道的钙稳态紊乱。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AChE 抗体 (A-11) 参考文献:

  1. 乙酰胆碱酯酶在阿尔茨海默病发病机制中的作用  |  Rees, TM. and Brimijoin, S. 2003. Drugs Today (Barc). 39: 75-83. PMID: 12669110
  2. 在 COS 细胞中表达的鱼雷乙酰胆碱酯酶的 H 和 T 亚基可生成各种类型的球状物。  |  Duval, N., et al. 1992. J Cell Biol. 118: 641-53. PMID: 1639848
  3. 终止子及其他:作为突触传递调节剂的乙酰胆碱酯酶》。  |  Zimmerman, G. and Soreq, H. 2006. Cell Tissue Res. 326: 655-69. PMID: 16802134
  4. 参与亚基间二硫键连接的牛脑乙酰胆碱酯酶主序列。  |  Roberts, WL., et al. 1991. J Biol Chem. 266: 7481-7. PMID: 2019579
  5. 从蛋白到肽:乙酰胆碱酯酶的非水解功能谱。  |  Halliday, AC. and Greenfield, SA. 2012. Protein Pept Lett. 19: 165-72. PMID: 21933122
  6. 乙酰胆碱酯酶抑制剂:药理学和毒理学。  |  Colović, MB., et al. 2013. Curr Neuropharmacol. 11: 315-35. PMID: 24179466
  7. 乙酰胆碱酯酶-Fc 融合蛋白(AChE-Fc): 延长血浆半衰期的新型潜在有机磷生物清除剂。  |  Noy-Porat, T., et al. 2015. Bioconjug Chem. 26: 1753-8. PMID: 26121420
  8. 乙酰胆碱酯酶与人类癌症。  |  Richbart, SD., et al. 2021. Adv Cancer Res. 152: 1-66. PMID: 34353435
  9. 哺乳动物神经肌肉突触处乙酰胆碱酯酶 mRNA 的神经调控。  |  Michel, RN., et al. 1994. J Cell Biol. 127: 1061-9. PMID: 7962068
  10. 大鼠乙酰胆碱酯酶亚基的克隆与表达:多种分子形式的产生以及与鱼雷胶原亚基的互补性。  |  Legay, C., et al. 1993. J Neurochem. 60: 337-46. PMID: 8417155
  11. 编码大鼠乙酰胆碱酯酶糖脂锚定形式的 cDNA 的表达。  |  Legay, C., et al. 1993. FEBS Lett. 315: 163-6. PMID: 8417973
  12. 阿尔茨海默氏症的淀粉样 beta 蛋白通过增加胚胎癌 P19 细胞的细胞内钙来增加乙酰胆碱酯酶的表达。  |  Sberna, G., et al. 1997. J Neurochem. 69: 1177-84. PMID: 9282941

订购信息

产品名称产品编号规格价格数量收藏夹

AChE 抗体 (A-11)

sc-373901
200 µg/ml
RMB2377.00

AChE (A-11): m-IgG Fc BP-HRP 套装

sc-525926
200 µg Ab; 10 µg BP
RMB2662.00

AChE (A-11): m-IgG2a BP-HRP 套装

sc-546461
200 µg Ab; 10 µg BP
RMB2662.00

AChE 抗体 (A-11) AC

sc-373901 AC
500 µg/ml, 25% agarose
RMB3129.00

AChE 抗体 (A-11) HRP

sc-373901 HRP
200 µg/ml
RMB2377.00

AChE 抗体 (A-11) FITC

sc-373901 FITC
200 µg/ml
RMB2482.00

AChE 抗体 (A-11) PE

sc-373901 PE
200 µg/ml
RMB2580.00

AChE 抗体 (A-11) Alexa Fluor® 488

sc-373901 AF488
200 µg/ml
RMB2685.00

AChE 抗体 (A-11) Alexa Fluor® 546

sc-373901 AF546
200 µg/ml
RMB2685.00

AChE 抗体 (A-11) Alexa Fluor® 594

sc-373901 AF594
200 µg/ml
RMB2685.00

AChE 抗体 (A-11) Alexa Fluor® 647

sc-373901 AF647
200 µg/ml
RMB2685.00

AChE 抗体 (A-11) Alexa Fluor® 680

sc-373901 AF680
200 µg/ml
RMB2685.00

AChE 抗体 (A-11) Alexa Fluor® 790

sc-373901 AF790
200 µg/ml
RMB2685.00

I see great results with this antibody on WB, but would like to validate my results with either siRNA or CRISPR studies. Which one would you recommend?

Asked by: TinTin
Great question! Both will knock down your gene of interested, however siRNA will inhibit the messenger RNA, giving you a temporary disabling/knockdown of the gene, while CRISPR will disable the gene itself and give you a permanent inactivation.
Answered by: Technical Support 15
Date published: 2017-02-09
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Rated 4 out of 5 by from きちんと見える冬にサンプルをいただき,発現の確認をしましたが,きちんと見ることができました。動物のタンパクを見るためには濃い濃度で使用する必要があると感じます。
Date published: 2019-05-07
Rated 1 out of 5 by from 条带位置不对试用装的批次做出来,在大鼠皮层神经元、大鼠脑组织、小鼠脑组织以及人类重组表达纯酶的WB条带,均在预期分子量可见清晰条带。买了正式包装后,结果显示不同样品的条带分子量位置有的在50KD,有的在90KD,都不在68KD附近。
Date published: 2018-12-19
Rated 5 out of 5 by from IF works well on rat liver frozen sectionRat liver frozen sections were subjected to immunofluorescence using AChE (A-11) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Positive signals in portal tracts were clear without any antigen retrieval.
Date published: 2018-03-14
Rated 5 out of 5 by from IHC staining of ACHE expression in Mouse BrainIHC staining of formalin fixed, paraffin-embedde mouse Brain showing best result. Application: Immunocytochemistry Sample :mouse Brain Antigen retrieval buffer: Citrate buffer pH6.0 Blocking step: 3%H2O2 for 10min at RT Fixative: Paraformaldehyde 1st Ab Incubation time: 18 hour(s) at 4°C ,and then 45min at 37°C· Diluent: 3%BSA Dilution: 1/100 Secondary antibody:Mouse two-step kit(PV-6002,Zsbio)
Date published: 2017-11-10
Rated 5 out of 5 by from Buen anticuerpo anti AChEEl anticuerpo reconoce AChE de muestras humanas de cerebro y LCR. También funciona perfectamente con cultivos celulares de la línea de neurobllastoma humano SH-SY5Y
Date published: 2017-10-20
Rated 5 out of 5 by from positive resultWe use Hela cell as positive control,the antibody quality confirmed no problem for WB detection. 1:800 for WB ,strong signal.
Date published: 2017-09-09
Rated 5 out of 5 by from 效果很好买了很久了才想起来评论,产品用的很顺利,条带很容易就做出来了,清晰稳定,价格又实惠,也推荐了其他人使用
Date published: 2017-05-11
Rated 5 out of 5 by from A good choice.This provides good AChE detection. Specific bands in WB and clear staining in IF. This is reactive with mouse, rat, and human.
Date published: 2017-01-24
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AChE 抗体 (A-11) is rated 4.5 out of 5 by 10.
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