Date published: 2025-10-26
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ACE Antibody (2E2) - Immunohistochemistry - Image 387524
ACE 抗体 (2E2): sc-23908. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞顶膜染色.
ACE 抗体 (2E2): sc-23908. 福尔马林固定, 石蜡包埋的人小肠组织的免疫过氧化物酶染色, 显示腺细胞顶膜染色.

ACE 抗体 (2E2): sc-23908

4.5(18)
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说明书
  • ACE 抗体 2E2 是小鼠单克隆 IgG1 κ，ACE抗体, 在37篇文献中引用，规格为200 µg/ml
  • 针对human肾脏血管紧张素转换酶
  • ACE 抗体 (2E2) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 ACE
  • 抗ACE抗体（2E2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 1 BP-HRP">m-IgG1 BP-HRP是ACE Antibody (2E2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与ACE Antibody (2E2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加ACE抗体，包括ACE2

ACE抗体（2E2）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对血管紧张素转换酶（ACE），也称为CD143、DCP1或血管紧张素转换酶1。ACE单克隆抗体（2E2）可通过多种应用有效检测小鼠、大鼠和人类来源的ACE蛋白，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。抗ACE抗体（2E2）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足各种研究需求。血管紧张素转换酶（ACE）是一种重要的羧基末端二肽外切酶，参与肾素-血管紧张素系统，ACE将血管紧张素I转化为强效血管收缩素激素血管紧张素II，在调节血压和电解质平衡方面发挥着重要作用。此外，ACE还能使血管收缩肽--缓激肽失活，从而维持体液和电解质的平衡。ACE有两种主要的同工型：肺ACE（ACEP）是一种在肺、肝、肾上腺皮质、胰腺、肾和脾脏的血管内皮细胞中表达的糖蛋白；睾丸ACE（ACET）仅在成年睾丸中由发育中的精子细胞（特别是晚期粗线粒体精子细胞）表达。了解ACE的功能对于研究心血管疾病、高血压及相关疾病至关重要，这使得抗ACE抗体（2E2）成为生物医学研究中不可或缺的工具。此外，虽然ACE在血管系统中普遍表达，但ACE2的表达模式更为有限，仅限于心脏、肾脏和睾丸细胞，这凸显了ACE（2E2）抗体精确检测的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ACE 抗体 (2E2) 参考文献:

  1. 一种新型血管紧张素转化酶相关羧肽酶（ACE2）可将血管紧张素 I 转化为血管紧张素 1-9。  |  Donoghue, M., et al. 2000. Circ Res. 87: E1-9. PMID: 10969042
  2. 利用替代性多腺苷酸化位点实现两种血管紧张素转换酶 mRNA 的组织特异性转录。  |  Thekkumkara, TJ., et al. 1992. Nucleic Acids Res. 20: 683-7. PMID: 1311831
  3. 血管紧张肽转化酶：血管内皮定位。  |  Caldwell, PR., et al. 1976. Science. 191: 1050-1. PMID: 175444
  4. 血管紧张肽转化酶与血管活性肽的调节。  |  Soffer, RL. 1976. Annu Rev Biochem. 45: 73-94. PMID: 183603
  5. 编码两种血管紧张素转换同工酶的 mRNA 是通过组织特异性选择的替代转录起始位点从同一基因中转录出来的。  |  Kumar, RS., et al. 1991. J Biol Chem. 266: 3854-62. PMID: 1847388
  6. 肾脏微粒体中的一种酶，能使缓激肽失活。  |  Erdos, EG. and Yang, HY. 1967. Life Sci. 6: 569-74. PMID: 4962200
  7. 兔睾丸二肽羧肽酶的分子和催化特性  |  El-Dorry, HA., et al. 1982. J Biol Chem. 257: 14128-33. PMID: 6754723
  8. 小鼠精子发生过程中睾丸血管紧张肽转化酶的表达调节。  |  Langford, KG., et al. 1993. Biol Reprod. 48: 1210-8. PMID: 8391328

订购信息

产品名称产品编号规格价格数量收藏夹

ACE 抗体 (2E2)

sc-23908
200 µg/ml
RMB2377.00

ACE (2E2): m-IgG1 BP-HRP 套装

sc-531641
200 µg Ab; 20 µg BP
RMB2662.00

ACE 抗体 (2E2) AC

sc-23908 AC
500 µg/ml, 25% agarose
RMB3129.00

ACE 抗体 (2E2) HRP

sc-23908 HRP
200 µg/ml
RMB2377.00

ACE 抗体 (2E2) FITC

sc-23908 FITC
200 µg/ml
RMB2482.00

ACE 抗体 (2E2) PE

sc-23908 PE
200 µg/ml
RMB2580.00

ACE 抗体 (2E2) Alexa Fluor® 488

sc-23908 AF488
200 µg/ml
RMB2685.00

ACE 抗体 (2E2) Alexa Fluor® 546

sc-23908 AF546
200 µg/ml
RMB2685.00

ACE 抗体 (2E2) Alexa Fluor® 594

sc-23908 AF594
200 µg/ml
RMB2685.00

ACE 抗体 (2E2) Alexa Fluor® 647

sc-23908 AF647
200 µg/ml
RMB2685.00

ACE 抗体 (2E2) Alexa Fluor® 680

sc-23908 AF680
200 µg/ml
RMB2685.00

ACE 抗体 (2E2) Alexa Fluor® 790

sc-23908 AF790
200 µg/ml
RMB2685.00

I used the antibody for IF on fixed (PFA 4%) adherent cells. I tried both RT and O/N 4°C incubation (1:50 dilution) but I didn't work, also changing secondary antibody. Same cells with Flow cytometry (SC23908 PE) resulted positive 90%. What should I do?

Asked by: angelavi
We are sorry to hear that our antibody is not yielding the expected results. Please contact SCBT technical service at [email protected] for help in this matter.
Answered by: Tech Support Europe
Date published: 2021-09-28

What is the recommended secondary reagent to use with ACE Antibody (2E2): sc-23908 for Western blot when working with human samples?

Asked by: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-24
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Rated 1 out of 5 by from not workingI got this antibody about 3 weeks ago and tested tissue staining (human kidney), cell staining and western blot. Unfortunately, nothing worked.
Date published: 2022-06-08
Rated 5 out of 5 by from Ottimo funzionamento!The sample worked very well in different cell lines.
Date published: 2021-05-12
Rated 4 out of 5 by from excellent跑wb哦 不错吧 效果很好 很容易跑, 推荐哦 这个抗体，提高实验效率了 哈哈
Date published: 2019-05-12
Rated 1 out of 5 by from Non funziona per WBHo provato questo anticorpo in diverse diluizioni e su diverse linee cellulari ma non funziona per Western Blot, nonostante diversi lavori con le versioni precedenti abbiano pubblicato WB funzionanti.
Date published: 2019-02-19
Rated 5 out of 5 by from strong signalGood quality and very cost-effective.It suitable for WB detection of ACE protein, ,We used 1:800 dilution,which can got strong signal.
Date published: 2017-09-02
Rated 5 out of 5 by from good result for ACEI use 6% acrylamide SDS-PAGE gel,and loading 60ug mouse kidney tissue , 100V for 2 hours.This condition may be very proper for this protein.The antibody incubate can be use for 1-2 hour or 4℃ overnight,both good result.
Date published: 2017-07-01
Rated 5 out of 5 by from 抗体效果良好前一段时间申请的该小样试用装，进免疫荧光鉴定后发现该抗体效果良好，可以做石蜡切片的荧光，基本无杂信号。
Date published: 2017-06-17
Rated 5 out of 5 by from 功能很出色条带很清晰, 结合力挺强,尽管不在预期的位置,总来说是个不错的抗体.santa的抗体便宜又好用,还会再买.
Date published: 2017-06-09
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ACE 抗体 (2E2) is rated 4.5 out of 5 by 18.
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