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ABCG2 Antibody (5D3) - Immunofluorescence - Image 145130
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
ABCG2 Antibody (5D3) - Immunofluorescence - Image 145130
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
ABCG2 Antibody (5D3) - Flow Cytometry - Image 6114
ABCG2 抗体 (5D3) FITC: sc-18841 FITC. HL-60/MX1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2b: sc-2857.
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.

ABCG2 抗体 (5D3): sc-18841

4.7(7)
寫評論提問

说明书
  • ABCG2抗体(5D3)是小鼠单克隆IgG2b κ, 在40篇文献中引用，规格为200 µg/ml
  • 针对转染了重组ABCG2的human来源的3T3细胞
  • ABCG2 抗体 (5D3) 推荐用于IP, IF 和 FCM，检测human 来源的 ABCG2
  • 抗ABCG2抗体（5D3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 2b BP-HRP">m-IgG2b BP-HRP是ABCG2 Antibody (5D3). 的首选辅助检测试剂。该试剂现与ABCG2 Antibody (5D3) 打包提供（请参阅下面的订购信息）。
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额外ABC抗体，包括ABCF1, ABCG2, ABCF2, ABCA6, ABCB9, ABCC13, ABCC11, ABCD3和ABCB5

ABCG2 抗体 (5D3) 是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫沉淀 (IP)、免疫荧光 (IF) 和流式细胞术 (FCM) 检测人类 ABCG2。ABCG2 (5D3) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ABCG2所属的ATP结合盒（ABC）转运蛋白家族在利用ATP水解产生的能量跨细胞膜转运分子方面发挥着关键作用。ABCG2，也称为胎盘特异性ABC转运蛋白和乳腺癌耐药蛋白（BCRP1），因其能够抵抗一系列化疗药物（如蒽环类、米托蒽醌、双蒽醌和拓扑替康）而显得尤为重要。这种抗药机制在癌症治疗中至关重要，因为ABCG2会影响化疗的效果。在正常生理条件下，ABCG2通过将毒素排出细胞发挥保护作用，并且是调节干细胞分化的关键因素。ABCG2负责侧群（SP）表型，在各种干细胞中广泛表达，因此ABCG2是重要的干细胞标记物。此外，ABCG2可能发生N-连接糖基化并在体内二聚化，这是重要的翻译后修饰，会影响ABCG2的功能和稳定性。ABCG2在胎盘、肝脏、肠道和干细胞中大量表达，这表明ABCG2在正常生理和疾病状态下具有多种功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ABCG2 抗体 (5D3)

sc-18841
200 µg/ml
RMB2422.00

ABCG2 (5D3): m-IgG2b BP-HRP 套装

sc-548650
200 µg Ab; 10 µg BP
RMB2715.00

ABCG2 抗体 (5D3) AC

sc-18841 AC
500 µg/ml, 25% agarose
RMB3189.00

ABCG2 抗体 (5D3) HRP

sc-18841 HRP
200 µg/ml
RMB2422.00

ABCG2 抗体 (5D3) FITC

sc-18841 FITC
200 µg/ml
RMB2527.00

ABCG2 抗体 (5D3) PE

sc-18841 PE
200 µg/ml
RMB2625.00

ABCG2 抗体 (5D3) Alexa Fluor® 488

sc-18841 AF488
200 µg/ml
RMB2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 546

sc-18841 AF546
200 µg/ml
RMB2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 594

sc-18841 AF594
200 µg/ml
RMB2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 647

sc-18841 AF647
200 µg/ml
RMB2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 680

sc-18841 AF680
200 µg/ml
RMB2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 790

sc-18841 AF790
200 µg/ml
RMB2738.00

Hello, as the sc-18841 is a mouse monoclonal IgG2b κ, should its molecular weight be around 150 kDa？

Asked by: AAAA
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-08

What is the molecular weight of this antibody? 

Asked by: EK1230
Thank you for your question. The observed molecular weight is 72kda and predicted molecular weight is 67kda.
Answered by: BlakeJ
Date published: 2022-11-14

What is the recommended fixation method for IF with ABCG2 (5D3): sc-18841 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by from Great FCM antibodyABCG2 (5D3) works great for IF and FCM. Would not recommend for Western blot (WB).
Date published: 2017-01-11
Rated 5 out of 5 by from We used this antibody in IF in human cancerWe used this antibody in IF in human cancer cells and got positive staining.
Date published: 2015-10-23
Rated 4 out of 5 by from IHC; published data on human retinal tumor samplesIHC; published data on human retinal tumor samples. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Great immunofluorescence membrane stainingGreat immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Works well with many different human cellWorks well with many different human cell lines: Eca109, Eca109/ABCG2, Eca109/PCDNA3.1 cells. -SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by from Great antibody for use in IHCGreat antibody for use in IHC, publishable data obtained using human brain tissue samples. -SCBT Publication Review
Date published: 2015-01-07
Rated 5 out of 5 by from Superb FCM data of HLSuperb FCM data of HL-60/MX1 cells. -SCBT QC
Date published: 2014-06-01
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ABCG2抗体 (5D3) is rated 4.7 out of 5 by 7.
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