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ABCG2 Antibody (5D3) - Immunofluorescence - Image 145130
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
ABCG2 Antibody (5D3) - Immunofluorescence - Image 145130
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.
ABCG2 Antibody (5D3) - Flow Cytometry - Image 6114
ABCG2 抗体 (5D3) FITC: sc-18841 FITC. HL-60/MX1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2b: sc-2857.
ABCG2 抗体 (5D3): sc-18841. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示膜定位.

ABCG2 抗体 (5D3): sc-18841

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寫評論提問
说明书
  • ABCG2抗体(5D3)是小鼠单克隆IgG2b κ, 在40篇文献中引用，规格为200 µg/ml
  • 针对转染了重组ABCG2的human来源的3T3细胞
  • ABCG2 抗体 (5D3) 推荐用于IP, IF 和 FCM，检测human 来源的 ABCG2
  • 抗ABCG2抗体（5D3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 2b BP-HRP">m-IgG2b BP-HRP是ABCG2 Antibody (5D3). 的首选辅助检测试剂。该试剂现与ABCG2 Antibody (5D3) 打包提供（请参阅下面的订购信息）。
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関連項目

额外ABC抗体，包括ABCF1, ABCG2, ABCF2, ABCA6, ABCB9, ABCC13, ABCC11, ABCD3和ABCB5

ABCG2 抗体 (5D3) 是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫沉淀 (IP)、免疫荧光 (IF) 和流式细胞术 (FCM) 检测人类 ABCG2。ABCG2 (5D3) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ABCG2所属的ATP结合盒（ABC）转运蛋白家族在利用ATP水解产生的能量跨细胞膜转运分子方面发挥着关键作用。ABCG2，也称为胎盘特异性ABC转运蛋白和乳腺癌耐药蛋白（BCRP1），因其能够抵抗一系列化疗药物（如蒽环类、米托蒽醌、双蒽醌和拓扑替康）而显得尤为重要。这种抗药机制在癌症治疗中至关重要，因为ABCG2会影响化疗的效果。在正常生理条件下，ABCG2通过将毒素排出细胞发挥保护作用，并且是调节干细胞分化的关键因素。ABCG2负责侧群（SP）表型，在各种干细胞中广泛表达，因此ABCG2是重要的干细胞标记物。此外，ABCG2可能发生N-连接糖基化并在体内二聚化，这是重要的翻译后修饰，会影响ABCG2的功能和稳定性。ABCG2在胎盘、肝脏、肠道和干细胞中大量表达，这表明ABCG2在正常生理和疾病状态下具有多种功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ABCG2 抗体 (5D3) 参考文献:

  1. Rab5效应因子EEA1是内体对接的核心组成部分。  |  Christoforidis, S., et al. 1999. Nature. 397: 621-5. PMID: 10050856
  2. 寡聚复合物将Rab5效应物与NSF连接起来，并通过EEA1和syntaxin 13之间的相互作用驱动膜融合。  |  McBride, HM., et al. 1999. Cell. 98: 377-86. PMID: 10458612
  3. 选择性膜招募的EEA1表明其在包被有蛋白酶体包膜的囊泡定向转运至早期内吞体中的作用。  |  Rubino, M., et al. 2000. J Biol Chem. 275: 3745-8. PMID: 10660521
  4. EEA1 FYVE指与磷脂酰肌醇3-磷酸和早期内吞体的相互作用。保守残基的作用。  |  Gaullier, JM., et al. 2000. J Biol Chem. 275: 24595-600. PMID: 10807926
  5. 使用Rab5亲和色谱和活性测定从牛脑细胞溶胶中纯化EEA1。  |  Christoforidis, S. and Zerial, M. 2001. Methods Enzymol. 329: 120-32. PMID: 11210528
  6. TGF beta 受体内化到 EEA1 富集的早期内体：在向 Smad2 发送信号中发挥作用。  |  Hayes, S., et al. 2002. J Cell Biol. 158: 1239-49. PMID: 12356868
  7. 在质膜上通过特定信号转导将EGF和转铁蛋白分拣到富含EEA1的内体中。  |  Leonard, D., et al. 2008. J Cell Sci. 121: 3445-58. PMID: 18827013
  8. 早期内体自体抗原1（EEA1）的C2H2锌指识别Rab GTP酶和系留内体的结构基础  |  Mishra, A., et al. 2010. Proc Natl Acad Sci U S A. 107: 10866-71. PMID: 20534488
  9. 感染巴西副球孢子菌的巨噬细胞中早期内体抗原1（EEA1）减少。  |  Voltan, AR., et al. 2013. Med Mycol. 51: 759-64. PMID: 23566224
  10. ESCRT-0标志着EGFR在细胞表面和EEA1阳性早期内体之间存在一条APPL1独立转运途径。  |  Flores-Rodriguez, N., et al. 2015. J Cell Sci. 128: 755-67. PMID: 25588841

订购信息

产品名称产品编号规格价格数量收藏夹

ABCG2 抗体 (5D3)

sc-18841
200 µg/ml
CNY2422.00

ABCG2 (5D3): m-IgG2b BP-HRP 套装

sc-548650
200 µg Ab; 10 µg BP
CNY2715.00

ABCG2 抗体 (5D3) AC

sc-18841 AC
500 µg/ml, 25% agarose
CNY3189.00

ABCG2 抗体 (5D3) HRP

sc-18841 HRP
200 µg/ml
CNY2422.00

ABCG2 抗体 (5D3) FITC

sc-18841 FITC
200 µg/ml
CNY2527.00

ABCG2 抗体 (5D3) PE

sc-18841 PE
200 µg/ml
CNY2625.00

ABCG2 抗体 (5D3) Alexa Fluor® 488

sc-18841 AF488
200 µg/ml
CNY2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 546

sc-18841 AF546
200 µg/ml
CNY2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 594

sc-18841 AF594
200 µg/ml
CNY2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 647

sc-18841 AF647
200 µg/ml
CNY2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 680

sc-18841 AF680
200 µg/ml
CNY2738.00

ABCG2 抗体 (5D3) Alexa Fluor® 790

sc-18841 AF790
200 µg/ml
CNY2738.00

Hello, as the sc-18841 is a mouse monoclonal IgG2b κ, should its molecular weight be around 150 kDa？

Asked by: AAAA
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-08

What is the molecular weight of this antibody? 

Asked by: EK1230
Thank you for your question. The observed molecular weight is 72kda and predicted molecular weight is 67kda.
Answered by: BlakeJ
Date published: 2022-11-14

What is the recommended fixation method for IF with ABCG2 (5D3): sc-18841 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Service 11
Date published: 2017-01-17
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Rated 5 out of 5 by Dr Ninau Qelp from Great FCM antibodyABCG2 (5D3) works great for IF and FCM. Would not recommend for Western blot (WB).
Date published: 2017-01-11
Rated 5 out of 5 by Harry from We used this antibody in IF in human cancerWe used this antibody in IF in human cancer cells and got positive staining.
Date published: 2015-10-23
Rated 4 out of 5 by SM from IHC; published data on human retinal tumor samplesIHC; published data on human retinal tumor samples. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by ET from Great immunofluorescence membrane stainingGreat immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by JM from Works well with many different human cellWorks well with many different human cell lines: Eca109, Eca109/ABCG2, Eca109/PCDNA3.1 cells. -SCBT Publication Review
Date published: 2015-02-21
Rated 5 out of 5 by HF from Great antibody for use in IHCGreat antibody for use in IHC, publishable data obtained using human brain tissue samples. -SCBT Publication Review
Date published: 2015-01-07
Rated 5 out of 5 by LR from Superb FCM data of HLSuperb FCM data of HL-60/MX1 cells. -SCBT QC
Date published: 2014-06-01
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ABCG2抗体 (5D3) is rated 4.7 out of 5 by 7.
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