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4E-BP1 Antibody (11G12C11) - Western Blotting - Image 17836
4E-BP1 抗体 (11G12C11): sc-81149. K-562 (A) 和 HL-60 (B) 全细胞裂解液中 4E-BP1 表达的 Western 印迹分析.
4E-BP1 抗体 (11G12C11): sc-81149. K-562 (A) 和 HL-60 (B) 全细胞裂解液中 4E-BP1 表达的 Western 印迹分析.

4E-BP1 抗体 (11G12C11): sc-81149

3.4(9)
寫評論提問

说明书
  • 4E-BP1抗体(11G12C11)是小鼠单克隆IgG1 κ, 在16篇文献中引用，规格为200 µg/ml
  • 针对与human起源的4E-BP1的N-terminal118个氨基酸对应的重组蛋白
  • 抗-4E-BP1 抗体 (11G12C11) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 4E-BP1
  • 抗4E-BP1抗体（11G12C11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取4E-BP1 (11G12C11): sc-81149的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 4E-BP1 抗体 (11G12C11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与4E-BP1 抗体 (11G12C11) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 4E-BP 抗体 包括 4E-BP1, 4E-BP2和4E-BP3

4E-BP1抗体（11G12C11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类样本中的4E-BP1。抗4E-BP1抗体（11G12C11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。4E-BP1作为翻译抑制剂，在调节蛋白质合成中起着至关重要的作用。4E-BP1与真核翻译起始因子4E（eIF-4E）结合，阻止翻译起始所必需的eIF-4F复合物的形成。4E-BP1被各种激酶（如S6激酶p70、MAP激酶或蛋白激酶C）磷酸化后，会与eIF-4E分离，从而促进对细胞生长和增殖至关重要的mRNA的翻译。这种调节机制在癌症生物学中尤为重要，因为蛋白质合成失调会导致肿瘤形成。4E-BP1还与4E-BP2相互作用，后者可调节eIF-4E的活性，这凸显了细胞过程中翻译控制的复杂性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

4E-BP1 抗体 (11G12C11)

sc-81149
200 µg/ml
RMB2377.00

4E-BP1 (11G12C11): m-IgGκ BP-HRP 套装

sc-521165
200 µg Ab, 40 µg BP
RMB2662.00

4E-BP1 抗体 (11G12C11) AC

sc-81149 AC
500 µg/ml, 25% agarose
RMB3129.00

4E-BP1 抗体 (11G12C11) HRP

sc-81149 HRP
200 µg/ml
RMB2377.00

4E-BP1 抗体 (11G12C11) FITC

sc-81149 FITC
200 µg/ml
RMB2482.00

4E-BP1 抗体 (11G12C11) PE

sc-81149 PE
200 µg/ml
RMB2580.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 488

sc-81149 AF488
200 µg/ml
RMB2685.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 546

sc-81149 AF546
200 µg/ml
RMB2685.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 594

sc-81149 AF594
200 µg/ml
RMB2685.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 647

sc-81149 AF647
200 µg/ml
RMB2685.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 680

sc-81149 AF680
200 µg/ml
RMB2685.00

4E-BP1 抗体 (11G12C11) Alexa Fluor® 790

sc-81149 AF790
200 µg/ml
RMB2685.00

I am using 4E-BP1 (11G12C11): sc-81149 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24

Does this antibody work equally in PBS and TBS?

Asked by: Muriel74
Thank you for your question. Yes, this product is provided in PBS, but will also work if diluted in TBS with 5% milk. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-12
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Rated 5 out of 5 by from Excellent antibody for western blottingReceived a sample and used it to probe by western blotting lysates of activated human leukocytes. Antibody worked very well. We detected two strong bands, and one faint third band. The same bands were also detected using the phospho 4E-BP1 (sc-271947) antibody.
Date published: 2020-03-11
Rated 2 out of 5 by from Works only on the unphosphorylated protein4E-BP1 protein is normally phosphorylated on several residues as part of it's regulation. at this state the antibody does not recognized the protein!!!! only after inhibiting mTOR and reducing the phosphorylation of the protein can it be recognized by the antibody
Date published: 2019-05-06
Rated 2 out of 5 by from Ok but weakThe antibody works but is very weak. Need a lot of it.
Date published: 2018-09-13
Rated 1 out of 5 by from Disappointing resultsIt took us ages to figure out why we weren't getting any signal for this antibody in western blot...finally I realized it was because it runs much higher than our other total and phospho 4E-BP1 antibodies, and we were consistently cutting off the bands. The variation, also, is much greater than for the rabbit total 4E-BP1 we have from CST, which is confusing. This variation makes me hesitant to use this antibody for any future work.
Date published: 2018-08-29
Rated 5 out of 5 by from Nice resultsNice and strong results produced with 4E-BP1 Antibody (11G12C11) by WB and IHCP
Date published: 2017-01-27
Rated 4 out of 5 by from Worked in IHCWorked in IHC
Date published: 2015-09-03
Rated 5 out of 5 by from Robust bands in western blot analysis of 4ERobust bands in western blot analysis of 4E-BP1 expression in K-562 and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Wonderful immunoperoxidase cytoplamsic stainingWonderful immunoperoxidase cytoplamsic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-04
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4E-BP1 抗体 (11G12C11) is rated 3.4 out of 5 by 9.
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