Date published: 2025-9-6

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Bcl-2 抗体 (100): sc-509

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说明书
  • Bcl-2抗体(100)是小鼠单克隆IgG1 κ, 在1,073篇文献中引用,规格为200 µg/ml
  • 与合成肽反应,该肽与human的Bcl-2的氨基酸41-54对应
  • Bcl-2 抗体 (100) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测human 来源的 Bcl-2; 不会与 Bcl-2 of mouse or rat origin产生交联反应
  • 抗Bcl-2抗体(100)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可与TRITC(sc-509 TRITC、200 µg/ml)或PerCP(sc-509 PerCP、)结合使用,用于IF、IHC(P)和FCM
  • 关于如何获取Bcl-2 (100): sc-509免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Bcl-2 抗体 (100) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Bcl-2 抗体 (100) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

快捷链接

関連項目

Bcl-2 抗体 (100) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和流式细胞术检测人类来源的 Bcl-2 蛋白。Bcl-2 单克隆抗体(100)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Bcl-2 作为细胞凋亡的关键调节因子发挥着至关重要的作用,而细胞凋亡对于正常发育、维持组织稳态和抵御病原体至关重要。作为一种抗凋亡的膜结合型癌蛋白,Bcl-2通过与Bcl-2家族的其他成员(包括Bcl-xL、Mcl-1、Bcl-w和A1等死亡抑制剂以及Bax、Bak、Bik、Bad和Bid等死亡诱导剂)进行蛋白质-蛋白质相互作用来促进细胞存活。Bcl-2的抗凋亡活性通过翻译后修饰进一步调节,包括蛋白水解加工和磷酸化,从而影响功能。通过稳定线粒体膜,Bcl-2可以抑制细胞色素c/Apaf-1途径的激活,从而防止细胞凋亡。Bcl-2的失调通常是由于Bcl-2基因突变引起的,它通过破坏细胞程序性死亡的正常机制导致各种癌症,这凸显了这种蛋白质在细胞健康和疾病中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Bcl-2 抗体 (100)

sc-509
200 µg/ml
RMB2377.00

Bcl-2 (100): m-IgGκ BP-HRP 套装

sc-520418
200 µg Ab, 40 µg BP
RMB2662.00

Bcl-2 抗体 (100) AC

sc-509 AC
500 µg/ml, 25% agarose
RMB3129.00

Bcl-2 抗体 (100) HRP

sc-509 HRP
200 µg/ml
RMB2377.00

Bcl-2 抗体 (100) FITC

sc-509 FITC
200 µg/ml
RMB2482.00

Bcl-2 抗体 (100) PE

sc-509 PE
200 µg/ml
RMB2580.00

Bcl-2 抗体 (100) Alexa Fluor® 488

sc-509 AF488
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) Alexa Fluor® 546

sc-509 AF546
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) Alexa Fluor® 594

sc-509 AF594
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) Alexa Fluor® 647

sc-509 AF647
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) Alexa Fluor® 680

sc-509 AF680
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) Alexa Fluor® 790

sc-509 AF790
200 µg/ml
RMB2685.00

Bcl-2 抗体 (100) TRITC

sc-509 TRITC
200 µg/ml
RMB2407.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service
Date published: 2016-12-23
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Rated 5 out of 5 by from Excellent antibody for Flow Cytometry analysisUsed this antibody for flow cytometry analysis and worked perfectly every time in our samples. Highly recommend it.
Date published: 2023-03-07
Rated 4 out of 5 by from Good antibody for western blottingBcl‐2 antibody: sc‐509 is good quality for western blotting (dilute factor: 1:1000)
Date published: 2020-05-25
Rated 4 out of 5 by from WBThis antibody worked well for WB at 1:200 in lysis from the breast cancer cells.
Date published: 2019-08-24
Rated 5 out of 5 by from Excellent antibodyThe antibody shows a strong band at the expected molecular weight.
Date published: 2019-04-10
Rated 5 out of 5 by from 功能很出色!功能很出色!支持!赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞赞!
Date published: 2018-06-16
Rated 5 out of 5 by from 功能很出色!研究生课题与Bax/Bcl-2相关,希望Santa cruz biotechnology能给初实验者一个免费赠送小样的机会,谢谢!
Date published: 2018-02-02
Rated 4 out of 5 by from Decent Bands, Consistent ResultsSample was ordered for the purpose of detecting BCL-2 and possible cleavage products. Was pleased to see clear bands at intended size with a 1:500 dilution. Used aliquot multiple times with consistent results.
Date published: 2018-01-04
Rated 5 out of 5 by from NICE resultsGood antibody.....strong fast signal. .... .
Date published: 2017-12-21
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Bcl-2 抗体 (100) is rated 4.7 out of 5 by 35.
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