Date published: 2025-9-6
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Bcl-2 Antibody (C-2) - Western Blotting - Image 379623
Bcl-2 抗体 (C-2): sc-7382. Raji (A), THP-1 (B), CCRF-CEM (C) 和 SUP-T1 (D) 全细胞裂解液中 Bcl-2 表达的 Western 印迹分析.
Bcl-2 抗体 (C-2): sc-7382. Raji (A), THP-1 (B), CCRF-CEM (C) 和 SUP-T1 (D) 全细胞裂解液中 Bcl-2 表达的 Western 印迹分析.

Bcl-2 抗体 (C-2): sc-7382

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说明书
  • Bcl-2 抗体 C-2 是小鼠单克隆 IgG1 κ，Bcl-2抗体, 在2,347篇文献中引用，规格为200 µg/ml
  • 与human的1-205氨基酸残基的Bcl-2
  • Bcl-2 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Bcl-2
  • 抗Bcl-2抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Bcl-2 (C-2): sc-7382的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Bcl-2 Antibody (C-2) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Bcl-2 Antibody (C-2) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Bcl-2抗体（C-2）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和流式细胞术检测小鼠、大鼠和人源Bcl-2蛋白。Bcl-2 单克隆抗体（C-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Bcl-2蛋白作为细胞凋亡的关键调节因子发挥着至关重要的作用，而细胞凋亡对于正常发育、维持组织稳态以及抵御病原体至关重要。对人类组织的免疫染色显示，Bcl-2主要定位于细胞质并与细胞膜相关联，这凸显了其作为抗凋亡癌蛋白的功能。Bcl-2通过与Bcl-2家族的其他成员（包括抗凋亡蛋白Bcl-xL、Mcl-1、Bcl-w和A1，以及促凋亡蛋白Bax、Bak、Bik、Bad和Bid）进行蛋白质-蛋白质相互作用来促进细胞存活。Bcl-2的抗凋亡功能可通过蛋白水解和磷酸化等机制进行调节，这些机制对其活性至关重要。此外，Bcl-2可通过稳定线粒体膜来抑制细胞色素c/Apaf-1途径的激活，从而防止细胞凋亡。Bcl-2基因的突变与多种癌症有关，正常凋亡过程会因Bcl-2抗凋亡效应的过度表达或失调而中断。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bcl-2 抗体 (C-2) 参考文献:

  1. 凋亡的 v-cyclin-CDK6 复合物使 Bcl-2 磷酸化并失活。  |  Ojala, PM., et al. 2000. Nat Cell Biol. 2: 819-25. PMID: 11056537
  2. 作为抗癌药物设计靶点的 Bcl-2 家族蛋白。  |  Huang, Z. 2000. Oncogene. 19: 6627-31. PMID: 11426648
  3. 过表达全长人BCL2可延长杆状病毒感染的Sf9昆虫细胞的存活时间。  |  Alnemri, ES., et al. 1992. Proc Natl Acad Sci U S A. 89: 7295-9. PMID: 1502141
  4. Bcl-2 家族成员在缺氧诱导的细胞死亡中的作用  |  Shroff, EH., et al. 2007. Cell Cycle. 6: 807-9. PMID: 17377500
  5. Bcl-2 是一种线粒体内膜蛋白，能阻止细胞的程序性死亡。  |  Hockenbery, D., et al. 1990. Nature. 348: 334-6. PMID: 2250705
  6. 细胞凋亡：一种对组织动力学具有广泛影响的基本生物现象。  |  Kerr, JF., et al. 1972. Br J Cancer. 26: 239-57. PMID: 4561027
  7. Bcl-2 与细胞程序性死亡的调控  |  Reed, JC. 1994. J Cell Biol. 124: 1-6. PMID: 8294493
  8. Bcl-2 阻止细胞凋亡：阻止细胞色素 c 从线粒体释放。  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
  9. Bcl-2 蛋白家族：细胞存活的仲裁者。  |  Adams, JM. and Cory, S. 1998. Science. 281: 1322-6. PMID: 9735050

订购信息

产品名称产品编号规格价格数量收藏夹

Bcl-2 抗体 (C-2)

sc-7382
200 µg/ml
RMB2377.00

Bcl-2 (C-2): m-IgGκ BP-HRP 套装

sc-520498
200 µg Ab, 40 µg BP
RMB2662.00

Bcl-2 (C-2): m-IgG1 BP-HRP 套装

sc-533466
200 µg Ab; 20 µg BP
RMB2662.00

Bcl-2 抗体 (C-2) AC

sc-7382 AC
500 µg/ml, 25% agarose
RMB3129.00

Bcl-2 抗体 (C-2) HRP

sc-7382 HRP
200 µg/ml
RMB2377.00

Bcl-2 抗体 (C-2) FITC

sc-7382 FITC
200 µg/ml
RMB2482.00

Bcl-2 抗体 (C-2) PE

sc-7382 PE
200 µg/ml
RMB2580.00

Bcl-2 抗体 (C-2) Alexa Fluor® 488

sc-7382 AF488
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) Alexa Fluor® 546

sc-7382 AF546
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) Alexa Fluor® 594

sc-7382 AF594
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) Alexa Fluor® 647

sc-7382 AF647
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) Alexa Fluor® 680

sc-7382 AF680
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) Alexa Fluor® 790

sc-7382 AF790
200 µg/ml
RMB2685.00

Bcl-2 抗体 (C-2) B

sc-7382 B
200 µg/ml
RMB2407.00

Bcl-2 抗体 (C-2) TRITC

sc-7382 TRITC
200 µg/ml
RMB2407.00

Bcl-2 抗体 (C-2) Alexa Fluor® 405

sc-7382 AF405
200 µg/ml
RMB2685.00

Dear, I am using your Bcl2 for immunofluorescence of mouse tissue. May I ask if there is a corresponding fluorescent secondary antibody available

Asked by: Tina1121
Thank you for your question. You can use the m-IgG κ BP-FITC, m-IgG κ BP-PE, m-IgG κ BP-CFL 488 and other fluorescent secondary antibodies. Thank you.
Answered by: Jiajun Z
Date published: 2023-06-05

Dear, I am interested in your antibody: anti-Bcl2 (c-2), ref sc-7382. Would it be possible to gel a small quantity of this product to try on my WB? Thank you for helping. Best wishes. Asta Valanciute

Asked by: Asta
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Service
Date published: 2022-08-10

请问做免疫细胞化学（爬片），用哪种bcl-2?

Asked by: zzzhang
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hello, do you know if this Anti-Bcl-2 polyclonal antibody (or any other) will work for Xenopus samples and/or for IP? Since a very big 1-205 aa sequence is mentioned as epitope I cannot actually blast to check for relevance. Thank you

Asked by: Alexpan2004
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

Hi, We're interested in cell death, including apoptosis and necrosis. And we want to get some preliminary data based on western blotting. Would you please recommend us some antibodies that would analyse cell deaths? Thanks, Ruizhi

Asked by: Ruizhi
Thank you for your question. There are so many proteins involved in apoptosis and necrosis that recommending specific proteins would not be possible for us to do without additional information. I suggest you take a look at necrosis and apoptosis marker proteins and decide on which ones you would like to study. Once you know which ones you want to look into, contact Technical Service again with your chosen protein targets and we're happy to help pick the best antibodies for those targets with you!
Answered by: Tech Service
Date published: 2019-11-12

请问这个抗体可以用于测试兔软骨的蛋白吗

Asked by: yall
Thank you for your question. This antibody has not yet been tested in rabbit and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates! 感谢您的提问。sc-7382还没有检测过兔子来源的样本，因此兔来源样本不是推荐检测的种属。
Answered by: Technical Support 9
Date published: 2019-10-23

Can I use this(monoclonal antibody against Bcl-2) mouse monoclonal antibody for immunohistochemical staining of swine(pig) tissue? Could you suggest me a suitable secondary antibody kit containing DAB chromogen?

Asked by: xgtian
Thank you for your question. We have not tested this antibody on swine or pig tissue, however, we are aware of a few publications citing use of this antibody in pig samples. For example, PMID: 19667935. We recommend using our ImmunoCruz® ABC Kit: sc-516216 , with our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to biotin (B), m-IgGκ BP-B: sc-516142 . The secondary antibody is sold separately from the kit. Alternatively, you could use the directly conjugated primary antibody, Bcl-2 Antibody (C-2) HRP: sc-7382 HRP.
Answered by: Technical Support
Date published: 2019-07-13

Does this antibody recognizes both BCL2 isoforms?

Asked by: Mari Francis
Thank you for your question. We do not have any data regarding the reactivity of this antibody against the two different isoforms of Bcl-2. This mouse monoclonal antibody was raised against amino acids 1-205 of Bcl-2 of human origin, corresponding to isoform alpha (accession number P10415). The exact epitope recognized has not been determined. The beta isoform of Bcl-2 has been described as mapping at the C-terminus between amino acids 196-239.
Answered by: Technical Support
Date published: 2019-04-05
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Rated 5 out of 5 by from Good antibodyUsed for Western blot, 1:1000, works well for the detection of endogenous and overexpressed BCL2.
Date published: 2025-08-21
Rated 5 out of 5 by from GoodGreat antibody for WB, works in mouse RAW 264.7 cells
Date published: 2025-03-18
Rated 5 out of 5 by from GoodIt's good to use. The band is clear and the backgroud is clean.
Date published: 2024-08-16
Rated 5 out of 5 by from Great features for western blotThe antibody can give a nice clean band. It is a good antibody.
Date published: 2024-07-05
Rated 3 out of 5 by from Resultado OptimoSe compro hace 3 meses y es funcional se tuvieron que ajustar las condiciones.
Date published: 2024-01-11
Rated 5 out of 5 by from Excellent functionalityI once used this antibody for Western blot experiments on mouse tumor tissue, and it worked very well
Date published: 2023-12-09
Rated 5 out of 5 by from GreatI use this antobody for Western Blot. I get a positive result. I would recommend this great antibody to my colleagues.
Date published: 2023-11-20
Rated 5 out of 5 by from Bcl-2 antibodyThe antibody is good at western blot. The band is clean and clear.
Date published: 2023-08-06
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Bcl-2 抗体 (C-2) is rated 4.6 out of 5 by 142.
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