Date published: 2025-9-10

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Phosphotyrosine 항체 (PY20): sc-508

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데이터 시트
  • Phosphotyrosine 항체(PY20) 는 마우스 monoclonal IgG2bκ, 1,325간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • p-Tyr에 대해 양성 반응
  • Phosphotyrosine 항체(PY20)는 broad species을 WB, IP, IF, IHC(P)와 FCM에 의해 검출하는 데 권장됩니다.
  • 항-Phosphotyrosine 항체(PY20)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Phosphotyrosine (PY20): sc-508무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 는 Phosphotyrosine항체 (PY20) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Phosphotyrosine 항체(PY20) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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p-Tyr 항체(PY20)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 절편을 이용한 면역조직화학(IHCP), 유세포분석법(FCM)을 통해 여러 종의 포스포티로신을 검출하는 마우스 단일클론 IgG2b 카파 경쇄 항체입니다. 항인산화티로신 항체(PY20)는 비접합 형태와 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 그리고 여러 알렉사 플루오르® 접합체를 포함한 다양한 접합체로 제공됩니다. 포스포티로신은 세포 신호 전달에 중요한 역할을 하는데, 특히 신호 전달 경로를 매개하는 단백질 티로신 키나아제를 조절하는 데 중요한 역할을 합니다. 이러한 키나아제는 수용체 티로신 키나아제와 비수용체 티로신 키나아제의 두 가지 주요 그룹으로 나뉘는데, Src 키나아제가 후자의 대표적인 예입니다. Src 키나아제는 일반적으로 세포 내 촉매 도메인이 없는 표면 수용체와 함께 신호 변환기 역할을 하는 내부 원형질막에 국한되어 있습니다. 수용체 티로신 키나아제는 14가지 계열에 걸쳐 50개가 넘는 구성원을 포함하고 있으며, 리간드 유도 티로신 인산화는 수용체 이량체화를 촉발하고 세포질 도메인 내의 특정 포스포티로신 잔기의 자가인산화를 촉발합니다. 이러한 인산화된 잔기는 세포질 신호 전달 분자의 결합 부위 역할을 하여 다양한 세포 과정에 영향을 미칩니다. 포스포티로신 단일클론 항체(PY20)는 포스포티로신을 함유하는 단백질을 특성화하고 정제하는 데 유용한 도구로 사용되어, 세포 신호 전달과 기능에 대한 이해를 증진시킵니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Phosphotyrosine 항체 (PY20)

sc-508
200 µg/ml
RMB2377.00

Phosphotyrosine (PY20): m-IgG Fc BP-HRP 번들

sc-528116
200 µg Ab; 10 µg BP
RMB2662.00

Phosphotyrosine (PY20): m-IgGκ BP-HRP 번들

sc-520417
200 µg Ab, 40 µg BP
RMB2662.00

Phosphotyrosine (PY20): m-IgG2b BP-HRP 번들

sc-549995
200 µg Ab; 10 µg BP
RMB2662.00

Phosphotyrosine 항체 (PY20) AC

sc-508 AC
500 µg/ml, 25% agarose
RMB3129.00

Phosphotyrosine 항체 (PY20) HRP

sc-508 HRP
200 µg/ml
RMB2377.00

Phosphotyrosine 항체 (PY20) FITC

sc-508 FITC
200 µg/ml
RMB2482.00

Phosphotyrosine 항체 (PY20) PE

sc-508 PE
200 µg/ml
RMB2580.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 488

sc-508 AF488
200 µg/ml
RMB2685.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 546

sc-508 AF546
200 µg/ml
RMB2685.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 594

sc-508 AF594
200 µg/ml
RMB2685.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 647

sc-508 AF647
200 µg/ml
RMB2685.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 680

sc-508 AF680
200 µg/ml
RMB2685.00

Phosphotyrosine 항체 (PY20) Alexa Fluor® 790

sc-508 AF790
200 µg/ml
RMB2685.00

Hello! Please tell me if p-Tyr Antibody (PY20): sc-508 is working in ELISA assay. If yes, could you recommend me a starting dilution range! I want to know if the p-Tyr Antibody (PY20) HRP variant is recommended for ELISA and in what dilution.

Asked by: Rodica
Thank you for your question. p-Tyr Antibody (PY20): sc-508 is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM. It is also available conjugated to HRP for ELISA. The starting dilution is 1:30, with a dilution range of 1:30-1:3000.
Answered by: Tech Support Europe
Date published: 2020-11-27

Which phosphotyrosine antibody is more sensitive in Western Blot: p-Tyr (PY99) or p-Tyr (PY20)? Which concentration is the recommended?

Asked by: AngelesMZ
Thank you for your question. Both p-Tyr Antibody (PY20): sc-508 and p-Tyr Antibody (PY99): sc-7020 are featured in several hundred publications and are recommended for western blot and other applications. Both antibodies have a recommended starting dilution of 1:200 and work in a dilution range 1:100-1:1000. Either antibody is perfectly suited for western blotting, so you could order one or the other or get in touch with our technical service to arrange for free samples of the two antibodies.
Answered by: Technical Support Europe
Date published: 2019-04-12

for blocking, what would you recommend? Many people recommend 5% of BSA for phospho-protein..( should I stick with this rule.). but some phospho-protein can worked well with.. Skim milk (depends the antibody protocol..)

Asked by: blessing
Thank you for your question. We recommend using 1% milk, 1% BSA in 0.05% Tween-20 for blocking and antibody incubations.
Answered by: Technical Support
Date published: 2019-02-01

EGF-treated とは、具体的にどのようにするものでしょうか。ご教授お願い致します。

Asked by: k 2018
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Answered by: Tech Service 6
Date published: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
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Rated 5 out of 5 by from Always reliable!I have relied on you for over 20 years and you have always delivered an excellent product on time. Your phone staff is friendly and helpful.
Date published: 2018-03-08
Rated 4 out of 5 by from Very goodI have used Py20 Antibody from Santa and it worked great.
Date published: 2017-12-14
Rated 4 out of 5 by from As expectedGives WB bands as expected at 1:500 dilution in 5% SA (block in non-fat dry milk).
Date published: 2017-12-02
Rated 5 out of 5 by from Very Strong SignalI compare different products for art antibody found p-Tyr antibody is the most sensitive, sc-508 is the most reliable quality.
Date published: 2017-10-28
Rated 5 out of 5 by from Excellent antibodyStrong signal and low background on immunoprecipitated protein.
Date published: 2017-09-01
Rated 5 out of 5 by from good result antibodyIt is an excellent antibody. gives a single band in my hand.
Date published: 2017-05-27
Rated 5 out of 5 by from antibody for WBI use the dilution 1:800, get very clean bands.I like
Date published: 2017-05-07
Rated 5 out of 5 by from High QualityGreat antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Date published: 2016-12-20
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Phosphotyrosine 항체 (PY20) is rated 4.8 out of 5 by 19.
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