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p-Ser/Phosphoserine 항체 (16B4): sc-81514

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  • p-Ser/Phosphoserine 항체 16B4 는 마우스 monoclonal IgM κ p-Ser/Phosphoserine 항체, 95간행물에 인용, 이며 50 µg/0.5 ml으로 제공합니다.
  • 포스포트레오닌에 대한 항체
  • WB와 IP으로 mouse, rat, human와 canine origin의 a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine을 검출할것을 권장합니다.
  • p-Ser/Phosphoserine (16B4): sc-81514의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 p-Ser/Phosphoserine 항체(16B4)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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p-Thr 와 p-Tyr를 위한 p-Ser항체

p-Ser 항체(16B4)는 마우스, 쥐, 인간 및 개 기원의 p-Ser 단백질을 WB 및 IP로 검출하는 IgM κ 마우스 단일 클론 p-Ser 항체(인산화 세린 항체, 포스포 세린 항체 또는 p-serine 항체로도 지정)입니다. p-Ser 항체(16B4)는 비접합 항-p-Ser 항체로도 사용할 수 있습니다. 단백질 키나아제는 표적 기질에서 세린, 트레오닌 또는 티로신 잔기의 인산화를 촉매하여 수많은 세포 신호 경로를 제어하는 메커니즘을 제공합니다. 세린/트레오닌 단백질 키나아제 계열의 Raf, Rsk, ROCK, PAK, Ak, PKC를 비롯한 여러 키나아제 계열이 표적 기질에서 세린과 트레오닌 잔기를 모두 인산화합니다. 인산화에 의한 단백질 변형은 단백질의 구조를 3차원적으로 변화시켜 효소 활성이나 다른 단백질과 상호 작용하는 능력을 변화시킬 수 있습니다. 포스포세린을 표적으로 하는 항체는 인산화 세린 잔기가 있는 단백질의 특성 분석과 세린 인산화와 관련된 세포 경로를 규명하는 데 사용될 수 있습니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

p-Ser/Phosphoserine 항체 (16B4) 참고문헌:

  1. ERK 유전자 산물을 인산화하는 단백질 키나아제인 MEK의 주요 구조.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. 인산세린 아미노트랜스퍼라제는 미생물에서 고등 진핵생물까지 약간의 차이를 제외하고 보존된 활성 부위를 가지고 있습니다.  |  Singh, RK., et al. 2021. Protein Pept Lett. 28: 996-1008. PMID: 33588715
  3. 포스포세린 포스파타제의 하향 조절은 종양 면역 환경을 강화하여 면역 체크포인트 차단 요법을 향상시킵니다.  |  Peng, ZP., et al. 2023. J Immunother Cancer. 11: PMID: 36849198
  4. 인산세린이 함유된 키토산 막은 내생 줄기세포를 활성화하여 뼈 재생을 촉진합니다.  |  Ke, Y., et al. 2023. Front Bioeng Biotechnol. 11: 1096532. PMID: 37034248
  5. 대장균에서 비가수분해성 인산세린의 재조합 단백질로의 생합성 및 유전자 인코딩.  |  Zhu, P., et al. 2023. Bio Protoc. 13: e4861. PMID: 37969748
  6. 과도한 포스포세린-129 α-시누클레인은 척추동물 시냅스에서 시냅스 소포의 이동과 클러스터 해제 결함을 유발합니다.  |  Wallace, JN., et al. 2024. Mol Biol Cell. 35: ar10. PMID: 37991902
  7. MEK 및 MKK 이소형에 의해 정의된 독립적인 인간 MAP-키나제 신호 전달 경로.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  8. 포유류 MAP 키나아제 키나아제(MEK1)의 구성 돌연변이 및 추정 조절 세린 인산화 부위.  |  Pagès, G., et al. 1994. EMBO J. 13: 3003-10. PMID: 8039496
  9. 미토겐 활성화 단백질 키나아제 및 90-kDa 리보솜 S6 키나아제에 의한 c-Fos 전사인자 억제 도메인의 인산화.  |  Chen, RH., et al. 1993. Proc Natl Acad Sci U S A. 90: 10952-6. PMID: 8248197
  10. 생쥐에서 Rho 관련 코일 코일 형성 단백질 세린/트레오닌 키나아제의 두 가지 동형체인 ROCK-I 및 ROCK-II.  |  Nakagawa, O., et al. 1996. FEBS Lett. 392: 189-93. PMID: 8772201
  11. 인간 Ste20 동족체인 hPAK1은 GTPase를 JNK MAP 키나제 경로에 연결합니다.  |  Brown, JL., et al. 1996. Curr Biol. 6: 598-605. PMID: 8805275
  12. 새로운 인산세린 포스파타제 억제제.  |  Hawkinson, JE., et al. 1997. Eur J Pharmacol. 337: 315-24. PMID: 9430431

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제품명카탈로그 번호 단위가격수량관심품목

p-Ser/Phosphoserine 항체 (16B4)

sc-81514
50 µg/0.5 ml
RMB2377.00

Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Asked by: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Answered by: Tech Support Europe
Date published: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Asked by: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2022-07-16

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Asked by: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Answered by: Tech Support Europe
Date published: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Asked by: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Answered by: Technical Support Europe
Date published: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Asked by: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Answered by: Tech Service
Date published: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Asked by: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Answered by: Tech Service
Date published: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Asked by: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Answered by: Technical Support
Date published: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Asked by: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 4 out of 5 by from p-Ser (16B4): sc-81514Western blot: Detecta multiples proteínas en extractos totales de células CRFK con 20 ug de proteína a una dilución de 1:500 y 1:1000 IP: Se utilizó 5 y 7 uL para inmunoprecipitar proteínas fosforiladas de células CRFK. Se utilizó la dilución de 1:500 para identificar las proteínas inmunoprecipitadas y sí se observan diversas bandas con diferentes pesos moleculares
Date published: 2024-04-01
Rated 5 out of 5 by from Works greatIt adequately detects phosphoserine antibodies. Even though it has some background, you can clean it up by more TBS T20 washes.
Date published: 2024-03-28
Rated 1 out of 5 by from 使えなかった一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Date published: 2020-11-18
Rated 3 out of 5 by from Pretty good antibodyThe antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Date published: 2020-10-07
Rated 3 out of 5 by from Worked in WBphosphorylation was detected in Pull downed protein in WB
Date published: 2019-07-23
Rated 5 out of 5 by from Successful detectionSuccessful detection of Ser phosphorylation in immunoprecipitates
Date published: 2018-12-07
Rated 3 out of 5 by from P-Ser AntibodyCompared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Date published: 2018-02-14
Rated 3 out of 5 by from Nice antibodyWe used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Date published: 2017-11-13
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p-Ser/Phosphoserine 항체 (16B4) is rated 4.0 out of 5 by 24.
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