Date published: 2025-9-11

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OctA-Probe 항체 (F-tag-01): sc-51590

4.4(9)
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데이터 시트
  • OctA-Probe 항체(F-tag-01) 는 마우스 monoclonal IgG1κ, 19간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • 합성 트레오닌-인산화 펩타이드에 대해 양성 반응
  • , , 의 OctA (FLAG®)-tagged fusion proteins을 WB, IP와 IF에 의해 감지하는 데 권장됨
  • See OctA-Probe (H-5): sc-166355 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • OctA-Probe (F-tag-01): sc-51590무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP는 OctA-Probe 항체(F-tag-01) WB 애플리케이션용입니다. 이 시약은 이제 OctA-Probe 항체(F-tag-01)와 함께 번들로 제공됩니다(아래 주문 정보 참조).

빠른 링크

더보기

tA 프로브 항체(F-tag-01)는 IgG1생쥐 단일 클론 OctA 프로브 항체(OctA 프로브 또는 FLAG 라벨 항체라고도 함)로 WB, IP, IF를 통해 OctA(FLAG®) 표기된 융합 단백질을 검출한다.이런 표현담체는 흔히 진핵표적단백질과 전문구역으로 구성된 교잡융합단백질을 코딩하는데 사용되는데 상술한 전문구역은 표적단백질의 순화와 시각화를 돕기 위해 설계되였다.8개의 아미노산으로 구성된 소친수펩타이드는 이미 여러가지 원핵과 진핵표현담체로 표현되는 단백질의 N말단으로 공정화되였다.이 작은 펩타이드는 표현에 사용할 수있는 융합 단백질의 시각화 및 면역 친화적 순화로 입증되었으며 펩타이드 부분의 작은 크기와 친수성으로 인해 표현 된 단백질은 종종 높은 수준의 생물학적 활성을 유지합니다.또 8개의 아미노산 부분은 장 효소로 절단해 제거할 수 있다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

주문정보

제품명카탈로그 번호 단위가격수량관심품목

OctA-Probe 항체 (F-tag-01)

sc-51590
100 µg/ml
RMB2377.00

OctA-Probe (F-tag-01): m-IgG Fc BP-HRP 번들

sc-536849
100 µg Ab; 10 µg BP
RMB2662.00

OctA-Probe (F-tag-01): m-IgGκ BP-HRP 번들

sc-534016
100 µg Ab; 40 µg BP
RMB2662.00

OctA-Probe (F-tag-01): m-IgG1 BP-HRP 번들

sc-544856
100 µg Ab; 20 µg BP
RMB2662.00

At what molecular weight should the band be seen in Western Blot with OctA-Probe (F-tag-01): sc-51590 monoclonal antibody?

Asked by: jenni
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect, equal to the protein of interest plus the weight of the OctA tag that it is fused to.
Answered by: Technical Support 9
Date published: 2017-01-17
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Rated 3 out of 5 by from Poor signal in yeast lysateThis did not work as well as the commercial Ab I use from a different company against a FLAG-tagged protein in budding yeast lysate. A very faint band could be detected but was not as bright as what I see with the other company's Ab.
Date published: 2018-02-12
Rated 5 out of 5 by from Hight titer antibodyThis antibody reacts specific to OctA-Probe.We uset 1:800 dilution by loading cell lysates 60ug.
Date published: 2018-01-27
Rated 3 out of 5 by from Western BlotUsed trial in Western blot looking at transfected HeLa lysate, but found non-specific band a little bit above 75kDa.
Date published: 2017-10-19
Rated 5 out of 5 by from Good Probe AntibodyOctA-Probe (F-tag-01) works great for Western blot (WB) in detecting OctA tagged recombinant fusion protein.
Date published: 2017-01-14
Rated 5 out of 5 by from Get good resultsGet good results for WB and IP in 293T cells. And the price was acceptable.
Date published: 2017-01-10
Rated 5 out of 5 by from Published data for WB in Dictyostelium discoideumPublished data for WB in Dictyostelium discoideum vegetative cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Published data for IP in HEK293 cellsPublished data for IP in HEK293 cells. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from Published data for WB in mouse bone marrowPublished data for WB in mouse bone marrow. -SCBT Publication Review
Date published: 2015-02-19
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OctA-Probe 항체 (F-tag-01) is rated 4.4 out of 5 by 9.
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