Date published: 2025-9-10

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CD47 항체 (B6H12): sc-12730

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데이터 시트
  • CD47 항체 B6H12 는 마우스 monoclonal IgG1 κ CD47 항체, 48간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • raised against intact CD47 purified from placenta
  • CD47 항체 (B6H12)는 WB, IP, IF, IHC(P) and FCM으로 and human유래의 the immunoglobulin domain of CD47 를 감지하는 데에 추천한다.
  • CD47 항체(B6H12)는 IP용으로 agarose에 결합된 형태로 제공됩니다. 그리고 IF, IHC(P) 및 FCM용으로 phycoerythrin 또는 FITC에 결합된 형태로 제공됩니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • CD47 (B6H12): sc-12730무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 CD47 항체 (B6H12) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은CD47 항체(B6H12) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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CD47 항체(B6H12)는 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 파라핀 포매 섹션 면역조직화학(IHCP), 유세포분석법(FCM)을 통해 인간 유래의 CD47 단백질을 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. CD47(B6H12) 항체는 비접합 형태와 아가로스, 피코에리틴(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체를 포함한 다양한 접합체 형태로 제공됩니다. CD47은 세포막을 가로지르는 양이온의 흐름을 조절하는 데 중요한 역할을 하는 통합막 단백질로, 특히 세포가 세포 외 기질에 부착하는 동안 세포 내 칼슘 농도의 증가에 영향을 미칩니다. CD47은 세포가 환경에 적절하게 반응하도록 돕기 때문에, 이 기능은 이동, 증식, 생존을 포함한 다양한 세포 과정에 필수적입니다. 또한, CD47은 세포 신호 전달과 통신에 중요한 역할을 하는 트롬보스폰딘의 C-말단 세포 결합 도메인에 대한 수용체 역할을 합니다. 특히, CD47은 Rh-null 적혈구에는 존재하지 않지만, 비적혈구 세포의 세포 부착에 크게 기여하며 순환에서 적혈구의 조기 제거를 방지할 수 있습니다. 또한, CD47은 조혈 세포, 상피 세포, 내피 세포, 섬유 아세포를 포함한 다양한 세포 유형에서 발현되며, 특히 뇌와 중간엽 세포에서 강하게 발현되어 정상적인 생리학 및 잠재적인 병리학 조건 모두에서 CD47의 중요성을 강조합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

CD47 항체 (B6H12) 참고문헌:

  1. 난소암종 관련 항원에 대한 단일 클론 항체, 낭종액으로 면역 접종하여 증가합니다.  |  Boerman, O., et al. 1989. Anticancer Res. 9: 551-8. PMID: 2764501
  2. 암 면역 요법을 위한 CD47 표적.  |  Veillette, A. and Chen, J. 2018. Trends Immunol. 39: 173-184. PMID: 29336991
  3. CD47-SIRPα 면역 체크포인트.  |  Logtenberg, MEW., et al. 2020. Immunity. 52: 742-752. PMID: 32433947
  4. 지방산 산화는 교모세포종 방사선 내성을 촉진하여 CD47 매개 면역 회피를 유발합니다.  |  Jiang, N., et al. 2022. Nat Commun. 13: 1511. PMID: 35314680
  5. CD47/SIRPα 축: 선천성 면역과 적응성 면역의 연결.  |  van Duijn, A., et al. 2022. J Immunother Cancer. 10: PMID: 35831032
  6. 난소암 항체의 이디오톱 구조 분석: 주로 중쇄 가변 서열이 다른 두 개의 단일 클론 항체가 동일한 에피토프를 인식합니다.  |  Slobbe, R., et al. 1994. Clin Exp Immunol. 98: 95-103. PMID: 7523009
  7. 정상 난소 세포와 유래 종양 간의 분화 마커의 발현 변화.  |  Van Niekerk, CC., et al. 1993. Am J Pathol. 142: 157-77. PMID: 7678716
  8. CD47 당단백질의 분리 및 특성 분석: 인테그린 관련 단백질(IAP) 및 난소 종양 마커 OA3와 동일한 다중 스패닝 막 단백질입니다.  |  Mawby, WJ., et al. 1994. Biochem J. 304 (Pt 2): 525-30. PMID: 7998989
  9. Rh 관련 항원 CD47은 신호 전달자인 인테그린 관련 단백질입니다.  |  Lindberg, FP., et al. 1994. J Biol Chem. 269: 1567-70. PMID: 8294396
  10. 인테그린 관련 단백질(CD47)은 CD3 활성화된 인간 T 세포의 코밋원성 분자입니다.  |  Ticchioni, M., et al. 1997. J Immunol. 158: 677-84. PMID: 8992983
  11. 신장 발암 물질인 니트릴로트리아세테이트 철로 처리된 쥐의 신장에서 인테그린 관련 단백질(CD47)의 과발현.  |  Nishiyama, Y., et al. 1997. Jpn J Cancer Res. 88: 120-8. PMID: 9119739

주문정보

제품명카탈로그 번호 단위가격수량관심품목

CD47 항체 (B6H12)

sc-12730
200 µg/ml
RMB2377.00

CD47 (B6H12): m-IgG Fc BP-HRP 번들

sc-528252
200 µg Ab; 10 µg BP
RMB2662.00

CD47 (B6H12): m-IgGκ BP-HRP 번들

sc-520582
200 µg Ab, 40 µg BP
RMB2662.00

CD47 (B6H12): m-IgG1 BP-HRP 번들

sc-542841
200 µg Ab; 20 µg BP
RMB2662.00

CD47 항체 (B6H12) AC

sc-12730 AC
500 µg/ml, 25% agarose
RMB3129.00

CD47 항체 (B6H12) FITC

sc-12730 FITC
200 µg/ml
RMB2482.00

CD47 항체 (B6H12) PE

sc-12730 PE
200 µg/ml
RMB2580.00

CD47 항체 (B6H12) Alexa Fluor® 488

sc-12730 AF488
200 µg/ml
RMB2685.00

CD47 항체 (B6H12) Alexa Fluor® 546

sc-12730 AF546
200 µg/ml
RMB2685.00

CD47 항체 (B6H12) Alexa Fluor® 594

sc-12730 AF594
200 µg/ml
RMB2685.00

CD47 항체 (B6H12) Alexa Fluor® 647

sc-12730 AF647
200 µg/ml
RMB2685.00

CD47 항체 (B6H12) Alexa Fluor® 680

sc-12730 AF680
200 µg/ml
RMB2685.00

CD47 항체 (B6H12) Alexa Fluor® 790

sc-12730 AF790
200 µg/ml
RMB2685.00

Hi, is this antibody specific to the extracellular domain for human CD47? I would like to stain frozen, formalin-fixed macaque tissue and am interested specifically in the extracellularly localized CD47. Thanks in advance!

Asked by: anonymous2
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-11-10

Hi, the concentration of the mAb, catalogue no. sc-12730 is stated as 100 microgram/mL. Can custom amount be ordered at higher concentration, what is the maximum amount and concentration?

Asked by: Anonymous
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for a custom concentration of sc-12730. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Tech Service 1
Date published: 2020-11-12

Is there an SDS for this product?

Asked by: BonnieK
Thank you for your question. Our antibody products are classified as non-hazardous items, so there is no SDS documentation for them.
Answered by: Tech Support Europe
Date published: 2020-08-19

A desperate client of mine with a dog with an intestinal tumor, cited a Standford U research that used CD47 for shrinking tumors. Is it available for sale? Dose? Cost?

Asked by: Dr B
Thank you for your question. Our antibodies are for research use only. They are not suitable for diagnostic or therapeutic use. Best of luck with your research.
Answered by: Technical Support
Date published: 2020-05-18

Bonjour , Serait-il possible de tester un échantillon de cet anticorps avant de le commander ? Merci

Asked by: Cath54
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-12-04

Does this antibody detect mouse CD47 in western blot? I thought it only detects human CD47 previously. You had MIAP301 for mouse. What have you changed in B6H12 antibody that it's able to detect mouse CD47?

Asked by: Kedar Ghimire
Thank you for your question. No, this antibody is specific for human CD47. We added mouse to the species reactivity based on a product citation using this antibody. However, we are going to remove mouse and only recommend this for human, unless we are able to get data in a mouse sample with this clone.
Answered by: Technical Support
Date published: 2019-04-13

Hi Can i use this antibody for flow Cytometric and western blot assay for the detection of CD47 on erythrocytes of Rhesus and Cynomolgus macaques?

Asked by: ashi_10123
Thank you for your question. This CD47 monoclonal antibody has been tested and published for Western blot and flow cytometry in human samples. We are not aware of any data or product citations in Rhesus and Cynomolgus macaques. Although, this CD47 clone B6H12 is recommended for detection of CD47 in primates, so it may also be suitable for these species.
Answered by: Technical Support
Date published: 2019-03-16

Is it know which epitope this antibody recognizes? Was it raised against a specific peptide (which one?) or fulll length protein (recombinant or purified?)? Thank you!

Asked by: babs440
Thank you for your question. This mouse monoclonal antibody was raised against intact CD47 purified from placenta. The exact epitope recognized by the monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2019-01-17
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Rated 4 out of 5 by from Good Antibodya good antibody, just some more band compare to what aspected. at the right molecular weight, we can see a clear band. primary diluted 1:500 and secondary 1:3000
Date published: 2023-01-17
Rated 4 out of 5 by from Works for FCMThe CD47 antibody works for flow cytometry on HeLa cells.
Date published: 2019-05-16
Rated 3 out of 5 by from Western blotThis antibody didn't had good performance in western blot (applied in gastric cancer cell line: AGS, MKN45), sometimes weak band and high background.
Date published: 2017-11-17
Rated 1 out of 5 by from it doesn't work in WBI have tested this antibody for WB (1:200) on whole cell lysates from HEK293, Jurkat and U251. Despite the datasheet shows a clear band around 49kDa, I could not detect any band in any of these cell lines. We tested several conditions and we can confirm the antibody doesn't work.
Date published: 2017-10-19
Rated 4 out of 5 by from Good antibody, strong signalGot this antibody as a trial antibody. Worked well on human airway epithelial cells (CFBE cells)
Date published: 2017-10-09
Rated 5 out of 5 by from Good for western blot in human cell linesI used this antibody before 3 days ago.The product shows good quality. The samples are protein of U937 cells treated with PMA and MDA-MB-231. Bands are clear in low background.
Date published: 2017-08-28
Rated 5 out of 5 by from good IHC stainingGood cytoplasmic staining with paraffin-embedded human adrenal gland tissue.
Date published: 2017-05-28
Rated 5 out of 5 by from perfect!The product shows good quality for western blot with human platelet extract
Date published: 2017-05-23
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CD47 항체 (B6H12) is rated 4.1 out of 5 by 15.
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