Date published: 2025-9-12

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beta Actin 항체 (AC-15): sc-69879

4.8(40)
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데이터 시트
  • beta Actin 항체 AC-15 는 마우스 monoclonal IgG1, 1,303간행물에 인용, 이며 100 µg/ml으로 제공합니다.
  • cytoplasmic β-Actin의 기원의 현탁액에 대해 양성 반응
  • WB, IP 와IF으로 broad species origin의 β-Actin을 검출할것을 권장합니다.; adult cardiac and skeletal muscle actins or detection in Dictyostelium discoideum 감지에 권장되지 않음
  • See beta Actin (C4): sc-47778 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • beta Actin (AC-15): sc-69879무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 beta Actin 항체(AC-15)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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β-액틴 항체(AC-15)는 세포질 β-액틴에 대응하는 약간 변형된 합성 펩타이드에 대해 양성된 마우스 단일 클론 IgG1 항체로, 웨스턴 블롯팅(WB), 면역 침전(IP) 및 면역 형광(IF)으로 광범위한 종의 β-액틴을 검출할 수 있는 항체이고, β-액틴을 검출할 수 있습니다. β-액틴은 세포 구조와 완전성을 유지하는 데 중요한 역할을 하는 고도로 보존된 단백질로 세포 골격의 주요 구성 요소로 작용하며 세포 운동성, 분열, 신호 전달 등 다양한 세포 과정에 참여합니다. β-액틴은 진핵 세포에서 전체 세포 단백질의 최대 50%를 차지하며 미세 융모 형성 및 근육 수축과 같은 기능에 필수적인 안정적이고 역동적인 필라멘트 구조를 형성합니다. β-액틴은 근육 수축을 위한 미오신과 액틴 필라멘트 중합 및 해중합을 조절하는 프로필린, 코필린, 겔솔린과 같은 액틴 결합 단백질 등 다양한 단백질과 상호 작용합니다. 또한 β-Actin은 Arp2/3 복합체와 결합하여 새로운 액틴 필라멘트의 핵 형성을 촉진하고 세포 골격 역학을 조절하는 Rho, Rac, Cdc42와 같은 작은 GTPase와 상호작용하며, Rho는 액틴 스트레스 섬유의 조립과 초점 접착을 제어하고, Rac는 원형질막에서 액틴 필라멘트 축적을 촉진하며, Cdc42는 필로포디아 형성을 자극합니다. 항-β-액틴 항체(AC-15)는 다양한 종의 액틴 역학, 세포 구조 및 관련 과정을 연구하는 연구자들에게 매우 유용한 도구입니다.

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제품명카탈로그 번호 단위가격수량관심품목

beta Actin 항체 (AC-15)

sc-69879
100 µg/ml
RMB2377.00

beta Actin (AC-15): m-IgG1 BP-HRP 번들

sc-551867
100 µg Ab; 20 µg BP
RMB2662.00

I didn't see (couldn't find yet) the list of species for which this reacts. Think it would work for molluscs? I need a loading control.....

Asked by: MoHU
Thank you for your question. We do not have a complete list of species reactivity for this β-Actin Antibody (AC-15): sc-69879. We know it is broadly reactive with mammalian and other non-mammalian species, however we are not aware specifically of any publication in mollusks.
Answered by: Technical Support
Date published: 2022-02-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Alternatively, SC-47778 is available directly conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-15

I need to detect beta-actin in bovine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2023-09-08
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Rated 2 out of 5 by from No hubo reconocimiento de la proteínaAnalicé este anticuerpo para el reconocimiento de la actina en celulas C6/36 pero no hubo formacion de la banda esperada
Date published: 2024-12-09
Rated 5 out of 5 by from 綺麗なシングルバンドが出ます1ヶ月前に初めて試しましたが、5μgの蛋白量、1/1000希釈、室温1時間、2次抗体は1/1000で綺麗なシングルバンドが出ました。
Date published: 2024-09-27
Rated 5 out of 5 by from Useful for both WB and IFNot only can it be used for Western blotting, but we also tried immuno cyto fluorescent staining with MeOH fixation and PFA fixation. With PFA fixation, the staining pattern, which is typical of monoclonal antibodies against the cytoskeleton, showed a dotted staining only where the epitope was exposed, but with MeOH fixation showed a fibrous staining pattern.
Date published: 2024-08-17
Rated 5 out of 5 by from Excellent!Clear and strong signal in WB of mouse neural cells.
Date published: 2024-03-29
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-20
Rated 5 out of 5 by from b-actin (sc-69879)It works well in western blot with 1:2000 dilution.
Date published: 2023-07-07
Rated 5 out of 5 by from SC-69879 - Anti -b Actin AC-15 in western blotWe are really satisfied of the Cod.SC-69879 - Anti -b Actin AC-15 Western Blot analysis in 10% SDS-PAGE of 40 μg of proteins from 4 brain tumour cell lines lysates. Anti-Actin Ab diluted 1:200; 4°C overnight.
Date published: 2022-08-11
Rated 5 out of 5 by from ExcellentI bought this antibody a few years ago and the result is good and clear.
Date published: 2021-11-08
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beta Actin 항체 (AC-15) is rated 4.8 out of 5 by 40.
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