Date published: 2025-9-11
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8-oxoG DNA Lesion Antibody (483.15) - Immunofluorescence - Image 142935
8-oxoG DNA 병변 항체(483.15): sc-130914. 핵 국소화를 보여주는 메탄올 고정 HeLa 세포의 면역 형광 염색.
8-oxoG DNA Lesion Antibody (483.15) - Immunofluorescence - Image 142935
8-oxoG DNA 병변 항체(483.15): sc-130914. 핵 국소화를 보여주는 메탄올 고정 HeLa 세포의 면역 형광 염색.
8-oxoG DNA 병변 항체(483.15): sc-130914. 핵 국소화를 보여주는 메탄올 고정 HeLa 세포의 면역 형광 염색.

8-oxoG DNA Lesion 항체 (483.15): sc-130914

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데이터 시트
  • 8-oxoG DNA Lesion 항체 483.15 는 마우스 monoclonal IgM κ 8-oxoG DNA Lesion 항체, 23간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 쥐 간에서 분리된 DOC 불용성 접합 리본에 대해 제기됨
  • IF와 ELISA으로 8-oxoG Lesions 을 검출할것을 권장합니다.
  • 8-oxoG DNA Lesion (483.15): sc-130914의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • 현재 8-oxoG DNA Lesion 항체(483.15)에 대해 선호하는 2차 검출 시약의 식별을 아직 완료하지 못했습니다. 이 작업은 진행 중입니다.

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8-oxoG DNA 병변 항체(483.15)는 8-oxoG DNA 병변 단백질을 검출하는 IgM κ 마우스 단일 클론 8-oxoG DNA 병변 항체(8-oxoG DNA 병변 항체로도 지정됨)입니다. 8-oxoG DNA 병변 항체(483.15)는 비접합 항 8-oxoG DNA 병변 항체로서 사용 가능합니다. DNA(디옥시리보핵산)는 알려진 모든 생물체와 일부 바이러스의 유전 물질입니다. 정보의 장기 저장과 발달 및 기능에 중요한 역할을 하는 DNA는 당 백본과 인산염 그룹을 포함하는 두 개의 긴 평행 뉴클레오티드 폴리머로 구성되어 있으며 에스테르 결합으로 서로 결합되어 이중 나선을 형성합니다. 8-oxoG(8-옥소구아닌)는 산화성 DNA 손상에 관여하는 돌연변이 유발성 병변입니다. 8-oxoG는 DNA 복제 중에 아데닌(A)과 잘못 결합하여 게놈 안정성에 위협을 가할 수 있습니다. 모든 유기체는 8-oxoG를 복구하기 위해 최소 두 가지 유형의 8-oxoG-DNA 글리코실라아제(OGG)를 발현합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

8-oxoG DNA Lesion 항체 (483.15) 참고문헌:

  1. 8-옥소구아닌 복구를 위한 여러 DNA 글리코실라제 및 생체 내 잠재적 기능.  |  Hazra, TK., et al. 2001. Prog Nucleic Acid Res Mol Biol. 68: 193-205. PMID: 11554297
  2. CHO 세포주에서 8-옥소구아닌 및 Ogg1-절개된 아퓨린 부위의 복구.  |  Boiteux, S. and le Page, F. 2001. Prog Nucleic Acid Res Mol Biol. 68: 95-105. PMID: 11554315
  3. 활성산소 과잉 생성에 취약한 노화 OXYS 쥐의 간세포에서 8-옥소구아닌 DNA 병변을 면역 형광으로 검출합니다.  |  Kemeleva, EA., et al. 2006. Mutat Res. 599: 88-97. PMID: 16574166
  4. 8-옥소구아닌의 위협에 대처하기 위한 다양한 DNA 복구 전략.  |  Russo, MT., et al. 2007. Mutat Res. 614: 69-76. PMID: 16769088
  5. 8-옥소구아닌에서의 전사 돌연변이 유발의 분자적 기초.  |  Damsma, GE. and Cramer, P. 2009. J Biol Chem. 284: 31658-63. PMID: 19758983
  6. 전기화학적 검출을 통한 ELISA 또는 고성능 액체 크로마토그래피(HPLC)를 이용한 산화적 DNA 손상 8-옥소구아닌(8-oxoG)의 측정.  |  Drake, DM., et al. 2019. Methods Mol Biol. 1965: 313-328. PMID: 31069684
  7. HDAC1은 노화된 뇌와 알츠하이머병에서 OGG1에 의한 산화성 DNA 손상 복구를 조절합니다.  |  Pao, PC., et al. 2020. Nat Commun. 11: 2484. PMID: 32424276
  8. 식물 텔로미어에서 8-oxoG의 정량화.  |  Castillo-González, C., et al. 2022. Int J Mol Sci. 23: PMID: 35563379
  9. 산화적 DNA 손상 8-OxoG를 종양 발생과 진행에 연결.  |  Zhao, Y., et al. 2022. Yi Chuan. 44: 466-477. PMID: 35729095
  10. 종양 발생 및 암 치료에서 8-oxoG 복구 시스템의 역할.  |  Li, C., et al. 2022. Cells. 11: PMID: 36497058
  11. 핵 추출물(SMADNE)에서 DNA 결합 단백질의 단일 분자 분석.  |  Schaich, MA., et al. 2023. Nucleic Acids Res. 51: e39. PMID: 36861323
  12. 인간 세포에는 두 가지 다른 8-옥소구아닌 복구 효소가 존재하며, 돌연변이를 예방하는 데 잠재적으로 상호 보완적인 역할을 합니다.  |  Hazra, TK., et al. 1998. Nucleic Acids Res. 26: 5116-22. PMID: 9801308

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8-oxoG DNA Lesion 항체 (483.15)

sc-130914
200 µg/ml
RMB2377.00

I am looking for an antibody that would be usable for flow cytometry quantification of 8-oxogunine DNA adducts -- WOULD THIS WORK? https://www.scbt.com/p/8-oxog-dna-lesion-antibody-483-15

Asked by: aporeminiscence
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-12

What is the recommended protocol for IF with this antibody? I tried methanol fixing (your standard guidelines) and IF with 1:50 dilution and standard secondary antibody, unfortunately didn' get any signal.

Asked by: annani
Thank you for your question. Please check out our recommended protocol here: https://www.scbt.com/de/resources/protocols/immunofluorescence-cell-staining If you are still having issues please contact our Technical Service team directly for support.
Answered by: Tech Support Europe
Date published: 2021-01-18

I want to use this antibody to detect 8-oxoG level on extracted mitochondrial DNA from c.elegans by performing dot blot. I'm wondering whether this antibody preferentially react to single stranded DNA and RNA. Do I need to denature my DNA sample?

Asked by: mike16
Thank you for your question. This is an antibody against the DNA lesion only as it will detect the 8-oxoG lesion itself. We recommend this antibody for ELISA and IF, not Western blotting, and no denaturing should be necessary.
Answered by: Tech Service
Date published: 2019-11-04

130914 8-oxoG is a IgM (Kappa light chain) why your company recommend use second ab is Anti mIgGk FITC or PE (516140 or 51614)? Do you have Anti mIGM k Ab? Can I you Anti mIGM ab as a second Ab?

Asked by: Mingz
Thank you for your question. We recommend the mouse IgG binding proteins for most IgM antibodies as they are fine for this use. If you have an anti-IgM secondary antibody, that will be fine to use as well.
Answered by: Tech Service
Date published: 2019-08-07

Does 8-oxoG DNA Lesion Antibody (483.15): sc-130914 react with bovine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with 8-oxoG DNA Lesion Antibody (483.15): sc-130914?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from good stainingStrong staining in IF, the dyeing effect was very good.
Date published: 2024-06-03
Rated 1 out of 5 by from Inconsistent from lot to lotI purchased a tube of anti-8-oxoG sc-130914 and optimized an immunofluorescence protocol using it in cultured human fibroblasts. The stain looked very good, and the signal was inducible by treatment with H2O2 or KBrO3 and repressible by treatment with Fpg. I used the whole tube for experiments and purchased a new tube, but the new tube performs terribly. It binds to all DNA so non-specifically that the signal is too strong to quantify even when the antibody is used at a dilution of 1 to 10,000 (I was using the first tube at 1 to 50). You might get lucky and get a good lot, but maybe you won't.
Date published: 2021-09-22
Rated 4 out of 5 by from IF LCLsHo utilizzato la 8-oxoG DNA lesion per IF su linfoblasti e si è rivelato un ottimo prodotto!
Date published: 2021-04-30
Rated 1 out of 5 by from No signal in IFI tested this antibody (purchased <2 months ago) on the cells subjected to 5mM, 1mM and 0 mM (negative control) H2O2 for 1 h twice: (1) using the standard methanol fixing procedure (without permeabilisaition) with buffers containing Triton X-100 0.3%; and (2) following the recommendation of SC Tech Support (https://www.scbt.com/de/resources/protocols/immunofluorescence-cell-staining) with the use of BSA as a blocking agent (the host of my secondary antibody routinely used in the lab is rabbit). In both experiments there's no signal above the 'no antibody' control. I am not an expert in this field but I have read that DNA denaturation is one of the steps of 5-mC IF procedure. Isn't it necessary also for detecting 8-oxoG?
Date published: 2021-01-22
Rated 3 out of 5 by from 8-oxo-G antibody for immunostaining8-oxo-G antibody works for immunostaining with human and porcine cultured cells that were fixed with methanol.
Date published: 2019-09-08
Rated 1 out of 5 by from RetinaI tried this antibody for mouse retina. it is not detecting for me
Date published: 2018-03-14
Rated 5 out of 5 by from GoodStrong staining in IF in HeLa cells, the dyeing effect was very good.
Date published: 2017-04-10
Rated 5 out of 5 by from Great IF StainingSuperb IF staining data; strong signal with low background
Date published: 2017-01-20
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8-oxoG DNA Lesion 항체 (483.15) is rated 3.6 out of 5 by 11.
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