Date published: 2025-9-16

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Anticorps Rad21 (H-12): sc-166973

5.0(4)
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Fiches techniques
  • L'Anticorps Rad21 (H-12) est un monoclonal de souris IgG2b κ, cité dans 3 publications, fourni en 200 µg/ml
  • soulevée à l'encontre des acides aminés 143-352 correspondant à une région interne de Rad21 d'origine human.
  • recommandé pour la détection de Rad21 d'origine mouse, rat et human par WB, IP, IF et ELISA
  • Le m-IgG Fc BP-HRP et le m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps Rad21 (H-12) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps Rad21 (H-12)(voir les informations relatives à la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Rad21 is one of the major cohesin subunits that holds sister chromatids together until anaphase, when proteolytic cleavage by separase, a caspase-like enzyme, allows chromosomal separation. RAD21 interacts with REC8 to form a cohesin complex that functions in sister chromatid alignment. RAD21 is also involved in the repair of double-strand breaks in DNA and is essential for mitotic growth. Rad 21 undergoes a C-terminal cleavage induced by diverse stimuli right before apoptosis. The cleavage product migrates to the cytoplasm and is involved in early events in the apoptotic pathway and it amplifiies the cell death signal in a positive-feedback manner. The RAD21 gene is related to the invasion and metastasis of cancer cells, and RAD21 is a potential target for cancer therapeutics that may enhance the anti-tumor activity of chemotherapeutic agents acting through the induction of DNA damage.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Rad21 (H-12)

sc-166973
200 µg/ml
RMB2377.00

Rad21 (H-12): m-IgG Fc BP-HRP Kit

sc-537554
200 µg Ab; 10 µg BP
RMB2662.00

Rad21 (H-12): m-IgGκ BP-HRP Kit

sc-534852
200 µg Ab; 40 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with Rad21 (H-12): sc-166973 monoclonal antibody?

Posée par: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par works good for WBtother with sc-271601 antibody this also works pretty nice.
Date de publication: 2017-11-30
Rated 5 de 5 de par Works with PFA FixationGreat for use in IF with PFA fixed samples, shows a bright signal localized to the nucleus.
Date de publication: 2017-02-02
Rated 5 de 5 de par Light band in WB in T24 and CaklLight band in WB in T24 and Cakl-1 whole cell lysates. -SCBT QC
Date de publication: 2015-03-19
Rated 5 de 5 de par Strong band in WB in HeLa and MOLTStrong band in WB in HeLa and MOLT-4 nuclear extracts. -SCBT QC
Date de publication: 2014-04-02
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Anticorps Rad21 (H-12) est évalué 5.0 de 5 de 4.
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