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Anticorps Munc13-4 (C-12): sc-271301

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Fiches techniques
  • L'Anticorps Munc13-4 (C-12) est un monoclonal de souris IgG2a (chaîne légère kappa) fourni en 200 µg/ml
  • dirigé contre les acides aminés 136-285 situés près de la région N-terminus de Munc13-4 d'origine human
  • recommandé pour la détection de Munc13-4 d'origine human par WB, IP, IF et ELISA
  • m-IgG Fc BP-HRP, 2a BP-HRP">m-IgG2a BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps Munc13-4 (C-12) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps Munc13-4 (C-12)(voir les informations de commande ci-dessous).

    ACCÈS RAPIDE AUX LIENS

    VOIR ÉGALEMENT...

    Munc13-4 is a member of the Munc13 family and is a homolog to Munc13-1. Munc13-4 lacks the C1 domain and N-terminal extension that are present in other Munc13 family members. It is a peripheral membrane, GTP-Rab 27a-binding protein. Munc13-4 has a ubiquitous tissue distribution, however, unlike related proteins Munc13-1, -2 and -3, Munc13-4 is mainly expressed outside the nervous system. High expression levels of Munc13-4 have been seen in mucous goblet and alveolar type II cells of the lung, as well as in cytotoxic T lymphocytes and mast cells. Munc13-4 localizes to secretory lysosomes. Overexpression of Munc13-4 enhances degranulation of mast cell secretory lysosomes, suggesting that it positively regulates secretory lysosome fusion and exocytosis. Mutations in Munc13-4 cause familial hemophagocytic lymphohistiocytosis subtype 3.

    Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

    Informations pour la commande

    Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

    Anticorps Munc13-4 (C-12)

    sc-271301
    200 µg/ml
    $316.00

    Munc13-4 (C-12): m-IgG Fc BP-HRP Kit

    sc-537605
    200 µg Ab; 10 µg BP
    $354.00

    Munc13-4 (C-12): m-IgGκ BP-HRP Kit

    sc-534920
    200 µg Ab; 40 µg BP
    $354.00

    Munc13-4 (C-12): m-IgG2a BP-HRP Kit

    sc-548064
    200 µg Ab; 10 µg BP
    $354.00

    What is the differences between this two products? Munc13-4 Antibody (C-12): sc-271301 and Munc13-4 Antibody (C-2): sc-271300? Which one should I choose for detection of UNC13D in NK cells??

    Posée par: Verna233
    Thank you for your question. The IgG2a Munc13-4 Antibody (C-12): sc-271301 was raised against amino acids 136-285 mapping near the N-terminus of Munc13-4 of human origin and is recommended for detection of Munc13-4 of human origin by WB, IP, IF and ELISA. The IgG1 Munc13-4 Antibody (C-2) was raised against the same amino acid sequence, but is also recommended for detection of Munc13-4 of human origin by IHC(P). They differ in their respective isotypes and recommeded applications and are otherwise quite similar. Either antibody is suitable for detection of human Munc13-4.
    Répondue par: Tech Support Europe
    Date de publication: 2019-11-11

    What is the recommended starting dilution for Western blot for Munc13-4 (C-12): sc-271301 monoclonal antibody?

    Posée par: jerojero
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-271301.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
    Répondue par: Technical Support
    Date de publication: 2017-03-01
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    Rated 5 de 5 de par Good Western blot data of Munc13Good Western blot data of Munc13-4 expression in non-transfected and human Munc13-4 transfected 293T whole cell lysates. -SCBT QC
    Date de publication: 2014-10-17
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    Anticorps Munc13-4 (C-12) est évalué 5.0 de 5 de 1.
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