Date published: 2026-4-1

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Anticorps eIF5 (A-3): sc-48419

5.0(1)
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Fiches techniques
  • L'Anticorps eIF5 (A-3) est un monoclonal de souris IgG2b κ, cité dans 2 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-300 de eIF5 d'origine human
  • recommandé pour la détection de eIF5 d'origine mouse, rat et human par WB, IP, IF et ELISA
  • m-IgG Fc BP-HRP, 2b BP-HRP">m-IgG2b BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps eIF5 (A-3) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps eIF5 (A-3)(voir les informations de commande ci-dessous).
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In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. The eukaryotic translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex. eIF-4E specifically binds to the mRNA cap to promote unwinding and exposure of the AUG-initiation codon. Overexpression of eIF-4E can lead to cell transformation and tumorigenesis. An additional initiation factor, eIF-2, is present as a heterotrimer composed of eIF-2a, eIF-2b and eIF-2g subunits. This heterotrimer forms a complex with GTP and tRNA which then binds to the 40S ribosomal subunit. After the formation of the 80S initiation complex, eIF-2 is hydrolyzed and eIF-2-GDP is released from the complex. eIF-2-GDP is subsequently converted to eIF-2-GTP, a reaction catalyzed by eIF-2B, and is then available to catalyze another round of initiation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps eIF5 (A-3)

sc-48419
200 µg/ml
CNY2422.00

eIF5 (A-3): m-IgG Fc BP-HRP Kit

sc-536839
200 µg Ab; 10 µg BP
CNY2715.00

eIF5 (A-3): m-IgGκ BP-HRP Kit

sc-534006
200 µg Ab; 40 µg BP
CNY2715.00

eIF5 (A-3): m-IgG2b BP-HRP Kit

sc-549835
200 µg Ab; 10 µg BP
CNY2715.00

What is the recommended starting dilution for Western blot for sc-48419: eIF5 (A-3) monoclonal antibody?

Posée par: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Répondue par: Technical Support
Date de publication: 2017-03-22
  • y_2026, m_4, d_1, h_12CST
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Rated 5 de 5 de par Great antibody for Western blotGreat antibody for Western blot! Positive data in A-431, BC3H1, NIH/3T3, 3611-RF and KNRK whole cell lysates. -SCBT QC
Date de publication: 2013-08-08
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Anticorps eIF5 (A-3) est évalué 5.0 de 5 de 1.
  • y_2026, m_4, d_1, h_12
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