Date published: 2025-9-7
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ZO-1 Antibody (R40.76) - Immunohistochemistry - Image 353766
ZO-1 抗体 (R40. 76): sc-33725. 福尔马林固定, 石蜡包埋的大鼠卵巢组织的免疫过氧化物酶染色显示卵泡细胞膜染色.
ZO-1 抗体 (R40. 76): sc-33725. 福尔马林固定, 石蜡包埋的大鼠卵巢组织的免疫过氧化物酶染色显示卵泡细胞膜染色.

ZO-1 抗体 (R40.76): sc-33725

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说明书
  • ZO-1 抗体 R40.76 是大鼠单克隆 IgG2a，ZO-1抗体, 在218篇文献中引用，规格为200 µg/ml
  • 对从大鼠肝脏分离的DOC不溶性连接带
  • ZO-1 抗体 (R40.76) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 ZO-1
  • 抗ZO-1抗体（R40.76）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ZO-1 (R40.76): sc-33725的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成ZO-1 抗体 (R40.76)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ZO 抗体 包括 ZO-1, ZO-2和ZO-3

ZO-1抗体（R40.76）是一种鼠单克隆IgG2a抗体，用于通过蛋白质印迹（WB）、免疫沉淀（IP）和PAR检测在小鼠、大鼠和人类样本中检测Zonula Occludens-1蛋白，也称为紧密连接蛋白1（TJP1）。抗ZO-1抗体（R40.76）是针对从大鼠肝脏中分离出的DOC不溶性连接带而制备的，可确保可靠识别ZO-1抗原决定簇。ZO-1是紧密连接的关键组成部分，对于维持上皮和内皮细胞层的完整性和选择性通透性至关重要。作为膜相关鸟苷酸激酶（MAGUK）家族的一员，ZO-1通过其PDZ、SH3和鸟苷酸激酶结构域与其他紧密连接蛋白相互作用，如封闭蛋白、钙粘蛋白、ZO-2和ZO-3，从而促进结构稳定性和信号转导。这些相互作用将紧密连接复合物与肌动蛋白细胞骨架联系起来，在调节细胞极性、屏障功能和细胞通讯方面发挥着至关重要的作用。ZO-1的失调与多种病理状况有关，包括癌症转移（其中许多乳腺癌细胞系中的ZO-1表达明显降低）以及心血管和神经系统疾病。抗ZO-1抗体（R40.76）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和Alexa Fluor®结合物，为不同的实验应用提供了多种选择。ZO-1 单克隆抗体（R40.76）使研究人员能够研究 ZO-1 的表达、定位和功能动态，从而增进我们对细胞连接及其在健康和疾病中的作用的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ZO-1 抗体 (R40.76) 参考文献:

  1. 确定 ZO-2 是与紧密连接和粘连连接相关的 MAGUK 家族成员，与闭塞素和 alpha catenin 具有亲和力。  |  Itoh, M., et al. 1999. J Biol Chem. 274: 5981-6. PMID: 10026224
  2. 紧密连接链内部和之间异质克劳丁的相互作用方式。  |  Furuse, M., et al. 1999. J Cell Biol. 147: 891-903. PMID: 10562289
  3. 紧密连接处的蛋白质相互作用。肌动蛋白有多个结合伙伴，ZO-1 与 ZO-2 和 ZO-3 形成独立的复合物。  |  Wittchen, ES., et al. 1999. J Biol Chem. 274: 35179-85. PMID: 10575001
  4. 三种紧密连接相关的 MAGUKs（ZO-1, ZO-2 和 ZO-3）与 claudins 的 COOH 端部直接结合。  |  Itoh, M., et al. 1999. J Cell Biol. 147: 1351-63. PMID: 10601346
  5. 闭塞素与 ZO-1 的直接关联及其可能参与闭塞素在紧密连接处的定位。  |  Furuse, M., et al. 1994. J Cell Biol. 127: 1617-26. PMID: 7798316
  6. 细胞信号神奇的 MAGUK  |  Anderson, JM. 1996. Curr Biol. 6: 382-4. PMID: 8723338
  7. ZO-3是紧密连接处发现的MAGUK蛋白家族的新成员，它与ZO-1和闭塞蛋白相互作用。  |  Haskins, J., et al. 1998. J Cell Biol. 141: 199-208. PMID: 9531559
  8. 乳腺癌中紧密连接蛋白 MAGUK ZO-1 的缺失：与腺体分化和杂合性缺失的关系。  |  Hoover, KB., et al. 1998. Am J Pathol. 153: 1767-73. PMID: 9846967

订购信息

产品名称产品编号规格价格数量收藏夹

ZO-1 抗体 (R40.76)

sc-33725
200 µg/ml
RMB2377.00

ZO-1 抗体 (R40.76) AC

sc-33725 AC
500 µg/ml, 25% agarose
RMB3129.00

ZO-1 抗体 (R40.76) HRP

sc-33725 HRP
200 µg/ml
RMB2377.00

ZO-1 抗体 (R40.76) FITC

sc-33725 FITC
200 µg/ml
RMB2482.00

ZO-1 抗体 (R40.76) PE

sc-33725 PE
200 µg/ml
RMB2580.00

ZO-1 抗体 (R40.76) Alexa Fluor® 488

sc-33725 AF488
200 µg/ml
RMB2685.00

ZO-1 抗体 (R40.76) Alexa Fluor® 546

sc-33725 AF546
200 µg/ml
RMB2685.00

ZO-1 抗体 (R40.76) Alexa Fluor® 594

sc-33725 AF594
200 µg/ml
RMB2685.00

ZO-1 抗体 (R40.76) Alexa Fluor® 647

sc-33725 AF647
200 µg/ml
RMB2685.00

ZO-1 抗体 (R40.76) Alexa Fluor® 680

sc-33725 AF680
200 µg/ml
RMB2685.00

ZO-1 抗体 (R40.76) Alexa Fluor® 790

sc-33725 AF790
200 µg/ml
RMB2685.00

When using formalin fixed paraffin embedded tissues that are on slides, which antigen retrieval should one use with this particular antibody?

Asked by: Song Lab
Thank you for your question. Please see section C of our protocol for antigen retrieval. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2025-06-05

What is the recommended dilution for ICC?

Asked by: Isabella
Thank you for your question. We have not tested the antibody for ICC and do not have a protocol.
Answered by: BlakeJ
Date published: 2025-02-07

ZO-1 (R40.76): sc-33725の無料の10 µgサンプルを使ってみたいのですがどのような手続きが必要ですか

Asked by: yoshimune
Thank you very much for your question. Please ask your Japanese dealer to apply for sample products. Website: www.cosmobio.co.jp ， www.iwai-chem.co.jp or www.nacalai.co.jp/ss/Contact/ 質問頂き、誠にありがとうございます。サンプル品の申請は日本のディーラーにお尋ねお願いします。ウェブサイト: www.cosmobio.co.jp ， www.iwai-chem.co.jp 又はwww.nacalai.co.jp/ss/Contact/
Answered by: Jiajun Z
Date published: 2025-02-05

如何看santa的应用是否可以用于冰冻切片的免疫荧光，IF还是IHC（Fr）

Asked by: fang
感谢您的提问。ZO-1 抗体 (R40.76): sc-33725推荐用于冰冻切片。推荐的应用IF表示此抗体可用于细胞爬片以及冰冻切片的免疫荧光。更多问题，欢迎通过：电话(+86) 021-60936351、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. ZO-1 antibody (R40.76): sc-33725 is recommended for IF with frozen tissue. Recommended application IF indicates that this antibody can be used for immunofluorescence of cells and frozen sections.If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936351 or [email protected] or by live chat on our website. Best of luck with your research!
Answered by: Sen Li
Date published: 2024-07-22

[At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZO-1 Antibody (R40.76). ]As stated above, what is the most recommended secondary antibody in WB and IHC(P) of rat intestinal tissue?

Asked by: yumi
Thank you for your question.ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a.A secondary antibody against rat IgG2a is recommended. Sorry, we do not have this product at this time, we recommend that you purchase it from other sources. Thank you.
Answered by: Jiajun Z
Date published: 2024-05-13

请问sc-33725（ZO-1）被不小心放进负二十度的冰箱里冻了一年，还可以再用吗？

Asked by: kadima
感谢您的提问。sc-33725应在4°C保存，不可以冷冻。冷冻后的抗体实验效果无法得到保证。 Thank you for your question. sc-33725 should be stored at 4°C, don't freeze. There is no warranty after the antibody is forzen.
Answered by: Technical Service SH
Date published: 2023-08-29

我想问一下ZO-1一抗有小鼠的吗

Asked by: liang32
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hi there, I would like to ask about ZO-1 Antibody (R40.76): sc-33725. Does this antibody detect both ZO-1α- and ZO-1α+ isoforms? or one of these isoforms? Many thanks!

Asked by: Shoma0308
For best assistance contact Technical Service.
Answered by: Tech Sales
Date published: 2021-07-09
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Rated 4 out of 5 by from Effective for staining mouse tissueI used it for staining the enamel epithelium of mouse tissue. It worked well with fluorescence staining.
Date published: 2025-04-02
Rated 1 out of 5 by from ZO-1 em intestino de camundongosBom dia, compramos este anticorpo há algumas semanas, foram feitos diferentes testes além do que consta no guia de protocolos. Não conseguimos detectar a banda refente ao ZO-1 (225kD). Estou enviando as imagens do "melhor" experimento que conseguimos até agora. Note que as bandas de beta-actina (42kD) estão ok, então acreditamos que não seja problema das nossas amostras uma vez que outros anticorpos,como E-caderina, estão funcionando normalmente. Gostaria de saber como podemos melhorar nosso protocolo de western blot. Obrigada
Date published: 2023-09-18
Rated 3 out of 5 by from Not great for IF as a plasma membrane markerThe antibody was used for immunofluorescence with secondary anti-rat antibody fused with Alexa647, in osteosarcoma cell lines U2OS and Saos2 and in WI-38 VA13 immortalized cell line. In U2OS and WI-38 VA13, the observed signal was not corresponding to the expected pattern, appearing as strong background with many faint foci, with most of the signal in the nucleus. In Saos2 cells, however, the signal was predominantly localized to intercellular boundaries, as expected, and in these cells, in our hands, this antibody could be used as a reliable marker of plasma membrane.
Date published: 2023-07-04
Rated 5 out of 5 by from ratingVery good antibodies, work flawlessly for immunofluorescence in the range of concentrations specified by the manufacturer.
Date published: 2023-07-03
Rated 4 out of 5 by from 功能很好如果能领到会很开心，这个抗体听说很好用，准备买这个，但可以先领用试用装，服务挺好的公司，非常值得大家使用
Date published: 2021-12-24
Rated 4 out of 5 by from 到货及时，功能突出上海圣克鲁斯厂家沟通起来非常顺畅，邮件回复很详细耐心，根据客户要求及时查货，订单确认后及时发货，实验结果也较为理想。
Date published: 2021-07-28
Rated 4 out of 5 by from work well on frozen tissue IFwe tested it on WB, Frozen tissue IF, and IHC-P. It worked well on frozen tissue IF, not that good on WB and IHC-P.
Date published: 2020-12-21
Rated 1 out of 5 by from scarsocomprato alcuni mesi fa, testato su linee cellulari, aspecifici da non consentire una corretta individuazione della proteina di interesse
Date published: 2019-11-25
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ZO-1 抗体 (R40.76) is rated 4.0 out of 5 by 49.
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