Date published: 2025-9-7
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Zic4 Antibody (RY-6) - Western Blotting - Image 34563
Zic4 抗体 (RY-6): sc-101202. HeLa 核提取物中 Zic4 表达的 Western 印迹分析.
Zic4 Antibody (RY-6) - Western Blotting - Image 34563
Zic4 抗体 (RY-6): sc-101202. HeLa 核提取物中 Zic4 表达的 Western 印迹分析.
Zic4 Antibody (RY-6) - Immunohistochemistry - Image 36337
Zic4 抗体 (RY-6): sc-101202. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色, 显示核定位.
Zic4 Antibody (RY-6) - Immunofluorescence - Image 35847
Zic4 抗体 (RY-6): sc-101202. 免疫荧光染色多聚甲醛固定的 HeLa 细胞, 显示核定位.
Zic4 抗体 (RY-6): sc-101202. HeLa 核提取物中 Zic4 表达的 Western 印迹分析.

Zic4 抗体 (RY-6): sc-101202

4.3(3)
寫評論提問

说明书
  • Zic4 抗体 RY-6 是小鼠单克隆 IgG2a κ，Zic4抗体, 在1篇文献中引用，规格为100 µg/1.0 ml
  • 免疫human物种的重组Zic4
  • 推荐用于 human 来源的Zic4 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Zic4 (RY-6): sc-101202的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Zic4 抗体 (RY-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Zic 抗体 包括 Zic1, Zic2, Zic3和Zic4

Zic4抗体（RY-6）是一种IgG2a κ小鼠单克隆Zic4抗体（也称为Zic4抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组化（IHC(P)）和酶联免疫吸附测定（ELISA）检测人类来源的Zic4蛋白。Zic4抗体（RY-6）为非偶联形式的抗Zic4抗体。Zic4（小脑锌指蛋白4）是一种C2H2锌指转录因子，影响小脑发育。Zic4定位于小脑颗粒细胞的细胞核。在小鼠发育中的小脑中，Zic4 mRNA的表达在出生后第5天达到峰值。Zic家族成员在发育过程中非常重要，并且与X连锁内脏异位和第5型全前脑畸形有关。Zic4与染色体3上的相关家族成员Zic1紧密相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Zic4 抗体 (RY-6) 参考文献:

  1. 转录因子 Zic1 和 Zic2 与载脂蛋白 E 基因启动子结合并产生转录活性。  |  Salero, E., et al. 2001. J Biol Chem. 276: 1881-8. PMID: 11038359
  2. Zic1 和 Zic2 突变小鼠的行为异常：作为人类神经系统疾病模型的意义。  |  Ogura, H., et al. 2001. Behav Genet. 31: 317-24. PMID: 11699604
  3. Zic1 通过与 Math1 增强子的相互作用以及对 Math1 自动调节的调节来抑制 Math1 的表达。  |  Ebert, PJ., et al. 2003. Development. 130: 1949-59. PMID: 12642498
  4. 关联基因 ZIC1 和 ZIC4 的杂合性缺失与丹迪-沃克畸形有关。  |  Grinberg, I., et al. 2004. Nat Genet. 36: 1053-5. PMID: 15338008
  5. 小鼠 Zic 基因家族新成员 Zic4 的鉴定和特征描述  |  Aruga, J., et al. 1996. Gene. 172: 291-4. PMID: 8682319
  6. 小鼠 Zic1, Zic2 和 Zic3 基因的表达表明，Zic 基因在体型形成过程中发挥着重要作用。  |  Nagai, T., et al. 1997. Dev Biol. 182: 299-313. PMID: 9070329

订购信息

产品名称产品编号规格价格数量收藏夹

Zic4 抗体 (RY-6)

sc-101202
100 µg/1.0 ml
RMB2504.00

Can sc-101202: Zic4 (RY-6) monoclonal antibody be used to perform WB in mouse samples? Which dilution do you recommend? (It says 1:100 in one literature https://www.nature.com/articles/s41598-024-71188-7, but it is used for human cancer cells)

Asked by: Joy8
Thank you for your question. sc-101202 is recommended for human samples. There is a 75% homology for mouse. We would not recommend using the antibody for mouse samples.
Answered by: BlakeJ
Date published: 2025-05-16

Have you validate CHIP for this antibody?

Asked by: Sasha
Thank you for your question. We have not validated sc-101202 Zic4 (RY-6) for Chip assay. the antibody is recommended for WB, IP, PAR, and ELISA.
Answered by: BlakeJ
Date published: 2025-03-27

Can sc-101202: Zic4 (RY-6) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-101202: Zic4 (RY-6) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for Western BlotAntibody detects a band at the expected molecular weight in HeLa nuclear extract. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Produced good immunoperoxidase nuclear stainingProduced good immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-02-03
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Zic4 抗体 (RY-6) is rated 4.3 out of 5 by 3.
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