Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
SIP1/ZEB2 Antibody (E-11) - Immunofluorescence - Image 145164
ZEB2 抗体 (E-11): sc-271984. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
SIP1/ZEB2 Antibody (E-11) - Immunofluorescence - Image 145164
ZEB2 抗体 (E-11): sc-271984. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
SIP1/ZEB2 Antibody (E-11) - Western Blotting - Image 375682
ZEB2 抗体 (E-11): sc-271984. 近红外 Western 印迹分析 EOC 20 全细胞裂解液中 ZEB2 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 790: sc-516181.
SIP1/ZEB2 Antibody (E-11) - Western Blotting - Image 138278
ZEB2 抗体 (E-11): sc-271984. 人脑组织提取物中 ZEB2 表达的 Western 印迹分析.
ZEB2 抗体 (E-11): sc-271984. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.

SIP1/ZEB2 抗体 (E-11): sc-271984

4.2(22)
寫評論提問

说明书
  • SIP1/ZEB2 抗体 E-11 是小鼠单克隆 IgG2a κ，SIP1/ZEB2抗体, 在80篇文献中引用，规格为200 µg/ml
  • 针对human起源的ZEB2内部区域内的氨基酸401-660进行
  • SIP1/ZEB2 抗体 (E-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ZEB2
  • 抗SIP1/ZEB2抗体（E-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SIP1/ZEB2 (E-11): sc-271984的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是SIP1/ZEB2 Antibody (E-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SIP1/ZEB2 Antibody (E-11) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ZEB2抗体（E-11）是一种IgG2a κ型鼠单克隆ZEB2抗体（也称为SIP1抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的ZEB2蛋白。ZEB2抗体（E-11）既可以是非偶联的抗ZEB2抗体形式，也可以是多种偶联形式的抗ZEB2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SMAD通过与不同类别的转录因子（包括DNA结合多锌指蛋白）相互作用来调节基因表达。ZEB2（锌指E盒结合蛋白2）是delta-EF1/Zfh1家族的双手锌指/同源域蛋白成员。ZEB2包含SMAD结合域、同源域以及位于N端和C端的两个锌指簇。ZEB2也称为SMADIP1、ZFHX1B和SIP1（SMAD相互作用蛋白1），可被TGFβ处理诱导。ZEB2在神经结构和神经嵴的正常胚胎发育中起关键作用。人类ZEB2基因定位于2号染色体q22。ZEB2基因突变会导致一种形式的Hirschsprung病（HSCR）。ZEB2突变患者表现出智力迟钝、运动发育迟缓、癫痫、小头畸形、独特的面部特征和/或先天性心脏病，所有这些都是HSCR的症状。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SIP1/ZEB2 抗体 (E-11) 参考文献:

  1. SIP1（Smad 交互蛋白 1）和 deltaEF1（delta-crystallin 增强子结合因子）是结构相似的转录抑制因子。  |  van Grunsven, LA., et al. 2001. J Bone Joint Surg Am. 83-A Suppl 1: S40-7. PMID: 11263664
  2. SIP1 Smad互作蛋白1的功能缺失突变导致综合征性赫氏病。  |  Cacheux, V., et al. 2001. Hum Mol Genet. 10: 1503-10. PMID: 11448942
  3. 在骨形态发生蛋白诱导的 C2C12 细胞成骨分化过程中，Smad-互作蛋白 1 是肝/骨/肾碱性磷酸酶转录的抑制因子。  |  Tylzanowski, P., et al. 2001. J Biol Chem. 276: 40001-7. PMID: 11477103
  4. 编码 Smad 互作蛋白 1 的 ZFHX1B 基因发生了无义突变和框架转换突变，导致了一种具有多种临床特征的复杂发育障碍。  |  Yamada, K., et al. 2001. Am J Hum Genet. 69: 1178-85. PMID: 11592033
  5. 中线结构受累的综合征赫氏prung 病中的大规模缺失和 SMADIP1 截断突变。  |  Amiel, J., et al. 2001. Am J Hum Genet. 69: 1370-7. PMID: 11595972
  6. 转录因子 ZEB2 是维持巨噬细胞组织特异性身份所必需的。  |  Scott, CL., et al. 2018. Immunity. 49: 312-325.e5. PMID: 30076102
  7. 内皮细胞Zeb2可维持肝血管结构，防止肝纤维化。  |  de Haan, W., et al. 2022. Cardiovasc Res. 118: 1262-1275. PMID: 33909875
  8. Zeb2增强子中的三重突变抑制了cDC2的发育。  |  Liu, TT., et al. 2022. Nature. 607: 142-148. PMID: 35732734
  9. 内皮细胞HDAC1-ZEB2-NuRD复合物通过调节蛋白质S-硫化的方式促进主动脉瘤和夹层的形成。  |  Luo, S., et al. 2023. Circulation. 147: 1382-1403. PMID: 36951067
  10. ZEB2和ACSL4之间的正反馈回路调节脂质代谢，促进乳腺癌转移。  |  Lin, J., et al. 2023. Elife. 12: PMID: 38078907
  11. 转录因子 ZEB2 驱动与年龄相关的 B 细胞形成。  |  Dai, D., et al. 2024. Science. 383: 413-421. PMID: 38271512
  12. 在自身免疫中，具有促进B细胞功能的年龄相关CD4+ T细胞受ZEB2调节。  |  Goto, M., et al. 2024. Sci Immunol. 9: eadk1643. PMID: 38330141

订购信息

产品名称产品编号规格价格数量收藏夹

SIP1/ZEB2 抗体 (E-11)

sc-271984
200 µg/ml
RMB2377.00

SIP1/ZEB2 (E-11): m-IgG Fc BP-HRP 套装

sc-525843
200 µg Ab; 10 µg BP
RMB2662.00

SIP1/ZEB2 (E-11): m-IgG2a BP-HRP 套装

sc-546437
200 µg Ab; 10 µg BP
RMB2662.00

SIP1/ZEB2 抗体 (E-11) AC

sc-271984 AC
500 µg/ml, 25% agarose
RMB3129.00

SIP1/ZEB2 抗体 (E-11) HRP

sc-271984 HRP
200 µg/ml
RMB2377.00

SIP1/ZEB2 抗体 (E-11) FITC

sc-271984 FITC
200 µg/ml
RMB2482.00

SIP1/ZEB2 抗体 (E-11) PE

sc-271984 PE
200 µg/ml
RMB2580.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 488

sc-271984 AF488
200 µg/ml
RMB2685.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 546

sc-271984 AF546
200 µg/ml
RMB2685.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 594

sc-271984 AF594
200 µg/ml
RMB2685.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 647

sc-271984 AF647
200 µg/ml
RMB2685.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 680

sc-271984 AF680
200 µg/ml
RMB2685.00

SIP1/ZEB2 抗体 (E-11) Alexa Fluor® 790

sc-271984 AF790
200 µg/ml
RMB2685.00

I'm thinking about using this antibody for immunohistochemistry. Could you tell me the condition of antigen retrieval? (HIER or PIER? etc)

Asked by: ttaa
Thank you for your question. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining We recommend antigen retrieval using sodium citrate and heat, but please look at the protocol for more information.
Answered by: Tech Service
Date published: 2019-01-07

I'm confused, in the datasheet it mentions that the size is 157kDa, but that is not the size in the western blots shown (at least not in one of them with three different lysates). Why is this? What is then the expected size we have to look for?

Asked by: HPer
Thank you for your question. According to Uniprot (http://www.uniprot.org/uniprot/O60315), the predicted molecular weight for ZEB2 is 136 kDa. On our product data sheet, we list 157 kDa as the expected molecular weight, which is also a prediction based on a peptide calculator. This is also consistent with the molecular weight that we observed when using this antibody for Western blot in human brain tissue extract. The Western blot data currently shown with three different lysates shows a signal detected between 90-132 kDa. Because we can not find any current reference supporting this molecular weight for ZEB2, we will remove this data from our website.
Answered by: Technical Support
Date published: 2022-11-27

I am going to use this antibody on my mouse cells. Any tips for how to do this with mouse monoclonal antibodies?

Asked by: Cweed
We recommend using conjugated primary antibodies. This product, sc-271984, is available directly conjugated to FITC, AF488, and AF 647.
Answered by: TechService7
Date published: 2016-12-30
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_271984, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 114ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from Not good.I got the free sample of ZEB2 antibody. The major band is at around 92kd, a weak band was shown at ~200kd size.However, the predict size should be ~136kd.
Date published: 2020-10-08
Rated 1 out of 5 by from Didn't work for usWe tried this sample several times, but unfortunately it didn't show any band for us.
Date published: 2018-11-13
Rated 1 out of 5 by from No band at appropriate positionWe bought the antibody ZEB2 (E-11) sc 271984 and since then we have performed several western blots with this antibody. However we have never seen a band at ~157kDa (the molecular weight provided in the data sheet). There are reports available for ZEB2 band at ~150kDa in the cells that we are using (dendritic cells) however we always see a band at ~75kDa. Although it is a monoclonal antibody we have not seen the band at the right position even once. I am putting up a picture for your referral with ZEB2 in control and ZEB2 knock down cells. We have ~75% knock down in transcript level however in western we do not observe any change.
Date published: 2018-02-06
Rated 5 out of 5 by from 功能很出色我申请了试用装，用于胰腺癌细胞ZEB2蛋白表达检测，下面是实验检测蛋白图，现特提出真实的图样，要求再购买使用装
Date published: 2018-01-22
Rated 2 out of 5 by from Concerned with the correct size.Received as a sample. Tested with 1:500 dilution on HeLa whole cell lysates. Detected a major band around 100 kDa (while in theory it should be around 140 kDa). Seems that other people also have similar findings. Is the detected main band truly ZEB2?
Date published: 2018-01-12
Rated 2 out of 5 by from PhDWe got a sample few months ago when we were at VCU (Requester Dongdong Wang). We have tried this antibody using total cell extracts of MDA231 and MCF10A cells with decent results. Although the western blot showed multiples bands.
Date published: 2017-12-22
Rated 4 out of 5 by from Good antibodyI useed this antibody in liver cell line. Two clear bands were observed. The minor band is 160KDa. The major band is 100KDa.
Date published: 2017-08-21
Rated 5 out of 5 by from solid choiceIt had powerful band detected in a large number of cell lines, clear band.
Date published: 2017-05-19
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_271984, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
SIP1/ZEB2 抗体 (E-11) is rated 4.2 out of 5 by 22.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_271984, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。