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ZEB1 Antibody (H-3) - Western Blotting - Image 364918
ZEB1 抗体 (H-3): sc-515797. WI-38 核提取物中 ZEB1 表达的 Western 印迹分析.
ZEB1 抗体 (H-3): sc-515797. WI-38 核提取物中 ZEB1 表达的 Western 印迹分析.

ZEB1 抗体 (H-3): sc-515797

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寫評論提問

说明书
  • ZEB1 抗体 H-3 是小鼠单克隆 IgG2a κ，ZEB1抗体, 在46篇文献中引用，规格为200 µg/ml
  • 免疫human物种的的氨基酸39-140
  • ZEB1 抗体 (H-3) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 ZEB1
  • 抗ZEB1抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-515797 X, 200 µg/0.1 ml)
  • 关于如何获取ZEB1 (H-3): sc-515797的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是ZEB1 Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ZEB1 Antibody (H-3) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

ZEB1抗体（H-3）是一种小鼠单克隆IgG2a kappa轻链抗体，旨在检测人类ZEB1，在包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）在内的多种应用中具有卓越的灵敏度和可靠性。抗ZEB1抗体（H-3）针对人类ZEB1的氨基酸39-140进行免疫，确保在涉及人类样本的实验中具有精确的靶向性和强烈的反应性。ZEB1，又称AREB6、BZP、DELTAEF1、FECD6、NIL2A、PPCD3、TCF8、ZFHEP和ZFHX1A，是一种关键的非受体转录因子，在胚胎发育过程中（尤其是中胚层组织）以及各种组织的成熟过程中，对基因表达的调节起着至关重要的作用。从结构上看，ZEB1包含两个对DNA结合和转录抑制至关重要的锌指结构域、一个参与蛋白质-蛋白质相互作用的同源结构域以及三个通过位点竞争和增强子沉默等机制调节基因表达的抑制结构域。ZEB1与多种关键蛋白相互作用，包括作为共抑制剂的C-末端结合蛋白（CtBP）和TGF-β信号传导通路中不可或缺的SMAD蛋白，从而影响上皮-间质转化（EMT）和细胞分化等过程。ZEB1与TSHβ基因T3反应元件的相互作用表明其在调节基因对甲状腺激素的特异性反应中发挥着重要作用，而甲状腺激素对于代谢和发育功能至关重要。除了在胚胎发生和组织发育中发挥基础作用外，ZEB1还参与骨骼结构形成的调节，并与多种生理和病理状况有关，包括癌症进展和纤维化。ZEB1单克隆抗体（H-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ZEB1 抗体 (H-3)

sc-515797
200 µg/ml
RMB2377.00

ZEB1 (H-3): m-IgG Fc BP-HRP 套装

sc-531517
200 µg Ab; 10 µg BP
RMB2662.00

ZEB1 (H-3): m-IgGκ BP-HRP 套装

sc-525222
200 µg Ab, 40 µg BP
RMB2662.00

ZEB1 (H-3): m-IgG2a BP-HRP 套装

sc-547808
200 µg Ab; 10 µg BP
RMB2662.00

ZEB1 抗体 (H-3) X

sc-515797 X
200 µg/0.1 ml
RMB2377.00

ZEB1 抗体 (H-3) AC

sc-515797 AC
500 µg/ml, 25% agarose
RMB3129.00

ZEB1 抗体 (H-3) HRP

sc-515797 HRP
200 µg/ml
RMB2377.00

ZEB1 抗体 (H-3) FITC

sc-515797 FITC
200 µg/ml
RMB2482.00

ZEB1 抗体 (H-3) PE

sc-515797 PE
200 µg/ml
RMB2580.00

ZEB1 抗体 (H-3) Alexa Fluor® 488

sc-515797 AF488
200 µg/ml
RMB2685.00

ZEB1 抗体 (H-3) Alexa Fluor® 546

sc-515797 AF546
200 µg/ml
RMB2685.00

ZEB1 抗体 (H-3) Alexa Fluor® 594

sc-515797 AF594
200 µg/ml
RMB2685.00

ZEB1 抗体 (H-3) Alexa Fluor® 647

sc-515797 AF647
200 µg/ml
RMB2685.00

ZEB1 抗体 (H-3) Alexa Fluor® 680

sc-515797 AF680
200 µg/ml
RMB2685.00

ZEB1 抗体 (H-3) Alexa Fluor® 790

sc-515797 AF790
200 µg/ml
RMB2685.00

I am using ZEB1(1:1000) for western Blotting. When I develop the blot ladder showed signal. What can I do?

Asked by: Kvy123
Thank you for your question. Please reach out to our technical service team so we can troubleshoot your experiment.
Answered by: Tech Support Europe
Date published: 2020-08-19

请问这个ZEB1 蛋白的大小多少？我看说明书上写的跟图不对。还有就是问一下，做WB时，稀释比例多少？装膜时间和转膜电流怎么控制？

Asked by: wbddd
感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。 Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

I am using ZEB1 (H-3): sc-515797 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from well function in Western blotVery good product, get a very beautiful WB band, recommended to many friends to buy, will continue to use it for WB in the future.
Date published: 2023-12-28
Rated 5 out of 5 by from Buon segnale e rapido sviluppoAbbiamo testato il campione per l'anticorpo monoclonale di topo per ZEB1 della Santa Cruz Biotechnology. Siamo soddisfatti delle performance dell’anticorpo. La membrana di nitrocellulosa con i nostri estratti proteici totali trasferiti su di essa è stata incubata per 1 ora con una soluzione di latte al 5% in TBS-T, per bloccare i siti di legame non specifici. Quindi, l'anticorpo primario sc-515797 è stato incubato over night a 4°C diluito 1:1000 in TBS-T con sodio azide allo 0,02%. Infine, l'anticorpo secondario anti-topo è stato incubato per 1 ora a temperatura ambiente diluito 1:2000 in soluzione di latte al 3% in TBS-T. Il segnale HRP è stato rilevato con l'incubazione ECL e lo sviluppo rapido della lastra autoradiografica. Il segnale è apparso come una singola banda specifica rilevata a circa 125kDa, come indicato nella scheda tecnica.
Date published: 2023-06-26
Rated 5 out of 5 by from ottimo funzionamentoHo acquistato questo prodotto e sono molto soddisfatta. Lo riacquisterò
Date published: 2022-04-01
Rated 4 out of 5 by from バンドが検出されます。WBで使用しました。非特異的なバンドが検出されますが問題ないと思います。目的のバンドはよりシャープなバンドとして検出されました。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 2 out of 5 by from NOT so goodThe best ZEB1 antibodies were the H102 and C20. Pity that they are no longer produced. The H3 is not so good. In WB gives a very weak signal (even a 1/50 dilution and it does not work in IHC/IF
Date published: 2019-10-11
Rated 4 out of 5 by from Great productI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 5 out of 5 by from greatThis antibody works great in breast cancer cell lines, and also cited in a published paper.
Date published: 2018-04-17
Rated 1 out of 5 by from no bandI use this antibody on nuclear extraction of pancreatic cell but got no band.
Date published: 2018-03-14
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ZEB1 抗体 (H-3) is rated 3.7 out of 5 by 25.
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