Date published: 2025-9-8

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ZBP1 抗体 (H-9): sc-271483

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说明书
  • ZBP1 抗体 H-9 是小鼠单克隆 IgG1 κ,ZBP1抗体, 在14篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于mouse物种的ZBP1的N-terminus
  • ZBP1 抗体 (H-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ZBP1
  • 抗ZBP1抗体(H-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-271483 X, 200 µg/0.1 ml)
  • 关于如何获取ZBP1 (H-9): sc-271483免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成ZBP1 抗体 (H-9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

ZBP1抗体(H-9)是一种IgG1 κ小鼠单克隆ZBP1抗体(也称为ZBP1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的ZBP1蛋白。ZBP1抗体(H-9)既有非偶联的抗ZBP1抗体形式,也有多种偶联形式的抗ZBP1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。左手Z-DNA是双螺旋的一种高能量形式。含有Za结构域的蛋白质具有特异性结合Z-DNA和/或Z-RNA的显著能力。ZBP1(Z-DNA结合蛋白1),也称为DLM-1,是一种含有429个氨基酸的蛋白质,其N端含有两个Za结构域拷贝,其中包含Za模体。ZBP1参与宿主对细胞应激的反应,包括肿瘤发生和病毒感染。它在淋巴组织中高表达,包括白细胞、淋巴结、扁桃体、骨髓、脾脏,在胸腺、肺和肝脏中也有少量表达。人类ZBP1有五种已知的异构体。ZBP1蛋白与小鼠和大鼠同源物的序列一致性分别为47%和46%。小鼠、大鼠和人类的ZBP1蛋白都含有四个保守区域,其中两个与RNA编辑酶ADAR1的Z-DNA结合域Za和Zb同源。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ZBP1 抗体 (H-9) 参考文献:

  1. 人类编辑酶 ADAR1 的 Zalpha 结构域与左手 Z-DNA 结合的晶体结构。  |  Schwartz, T., et al. 1999. Science. 284: 1841-5. PMID: 10364558
  2. 利用 RNA 差示技术克隆活化巨噬细胞中上调的新基因 DLM-1。  |  Fu, Y., et al. 1999. Gene. 240: 157-63. PMID: 10564822
  3. 人类 Z-DNA 结合蛋白 DLM-1 基因的复杂调控。  |  Rothenburg, S., et al. 2002. Nucleic Acids Res. 30: 993-1000. PMID: 11842111
  4. ZBP1:调节细胞死亡和炎症的先天传感器。  |  Kuriakose, T. and Kanneganti, TD. 2018. Trends Immunol. 39: 123-134. PMID: 29236673
  5. 流感病毒Z-RNA诱导ZBP1介导的坏死性细胞凋亡。  |  Zhang, T., et al. 2020. Cell. 180: 1115-1129.e13. PMID: 32200799
  6. ZBP1-NLRP3炎症小体的调节及其在热解、凋亡和坏死(PANoptosis)中的意义。  |  Zheng, M. and Kanneganti, TD. 2020. Immunol Rev. 297: 26-38. PMID: 32729116
  7. ADAR1 限制 ZBP1 介导的免疫反应和 PAN 细胞凋亡,从而促进肿瘤发生。  |  Karki, R., et al. 2021. Cell Rep. 37: 109858. PMID: 34686350
  8. 先天免疫、细胞死亡和疾病中的ADAR1和ZBP1。  |  Karki, R. and Kanneganti, TD. 2023. Trends Immunol. 44: 201-216. PMID: 36710220
  9. ZBP1介导的端粒-线粒体信号传导介导复制危机。  |  Nassour, J., et al. 2023. Nature. 614: 767-773. PMID: 36755096
  10. 无菌性炎症中的Z-核酸传感器ZBP1。  |  Song, Q., et al. 2024. Clin Immunol. 261: 109938. PMID: 38346464
  11. ZBP1通过诱导RIPK3介导的坏死凋亡和RIPK1激酶活性无关的凋亡来引起炎症。  |  Koerner, L., et al. 2024. Cell Death Differ. 31: 938-953. PMID: 38849574
  12. 左旋 Z-DNA 的化学和生物学。  |  Rich, A., et al. 1984. Annu Rev Biochem. 53: 791-846. PMID: 6383204

订购信息

产品名称产品编号规格价格数量收藏夹

ZBP1 抗体 (H-9)

sc-271483
200 µg/ml
RMB2377.00

ZBP1 抗体 (H-9) X

sc-271483 X
200 µg/0.1 ml
RMB2377.00

ZBP1 抗体 (H-9) AC

sc-271483 AC
500 µg/ml, 25% agarose
RMB3129.00

ZBP1 抗体 (H-9) HRP

sc-271483 HRP
200 µg/ml
RMB2377.00

ZBP1 抗体 (H-9) FITC

sc-271483 FITC
200 µg/ml
RMB2482.00

ZBP1 抗体 (H-9) PE

sc-271483 PE
200 µg/ml
RMB2580.00

ZBP1 抗体 (H-9) Alexa Fluor® 488

sc-271483 AF488
200 µg/ml
RMB2685.00

ZBP1 抗体 (H-9) Alexa Fluor® 546

sc-271483 AF546
200 µg/ml
RMB2685.00

ZBP1 抗体 (H-9) Alexa Fluor® 594

sc-271483 AF594
200 µg/ml
RMB2685.00

ZBP1 抗体 (H-9) Alexa Fluor® 647

sc-271483 AF647
200 µg/ml
RMB2685.00

ZBP1 抗体 (H-9) Alexa Fluor® 680

sc-271483 AF680
200 µg/ml
RMB2685.00

ZBP1 抗体 (H-9) Alexa Fluor® 790

sc-271483 AF790
200 µg/ml
RMB2685.00

请问这个抗体的二抗是什么呢?

Asked by: 11东方我
感谢您的提问,使用抗小鼠IgG的二抗。 Thank you for your question. Anti-mouse IgG secondary antibody can be used.
Answered by: Sen Li
Date published: 2025-08-07

Whether this antibody can be used for IF staining in mouse brain? Any successful examples?

Asked by: Lewis1993
Thank you for your inquiry. This antibody is recommended for detection of mouse origin by IF experiments in frozen sections, but not in paraffin sections. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2024-12-24

The bands are running much higher then the predicted 46KD. Have you confirmed with ZBP1-/- mice or siRNA to show specificity?

Asked by: Hskoehle
Thank you for your question. While the predicted weight is 46 kDa based on sequence, the published weights for ZBP1 range as high as 68 kDa. We do not have any KO data for this target at this time. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-06-27

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-271483: ZBP1 (H-9) mouse monoclonal antibody will work?

Asked by: Trav11
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
  • y_2025, m_8, d_30, h_9CST
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Rated 5 out of 5 by from 特异性很好我之前购买过,真的很好用,特异性很好,能跑出想要的趋势,是一个很不错的产品,值得购买,值得购买,值得购买
Date published: 2023-08-02
Rated 5 out of 5 by from 这个抗体还是不错滴之前一直苦于针对老鼠的ZBP1,购买了各种公式的均不好用,最后santa的确实很不错。要找到一个合适抗体太不容易了
Date published: 2021-10-12
Rated 5 out of 5 by from works very goodthis antibody works very good, I used as 1:500 dilution for WB, which can generate a specific strong band
Date published: 2017-09-12
Rated 5 out of 5 by from Works great in WBProduces strong clear and clean band in WB on EOC 20, RAW 264.7, M1 and RPE-J whole cell lysates at a 1:200 dilution.
Date published: 2017-01-31
Rated 5 out of 5 by from Produced nice Western blot data of ZBP1 expressionProduced nice Western blot data of ZBP1 expression in MCP-5 whole cell lysate. -SCBT QC
Date published: 2013-12-27
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ZBP1 抗体 (H-9) is rated 5.0 out of 5 by 5.
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