Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
YY1 Antibody (H-10) - Western Blotting - Image 137859
YY1 抗体 (H-10): sc-7341. BJAB (A), MCF7 (B), PC-3 (C), Jurkat (D), HL-60 (E) 和 K-562 (F) 核提取物中 YY1 表达的 Western 印迹分析.
YY1 抗体 (H-10): sc-7341. BJAB (A), MCF7 (B), PC-3 (C), Jurkat (D), HL-60 (E) 和 K-562 (F) 核提取物中 YY1 表达的 Western 印迹分析.

YY1 抗体 (H-10): sc-7341

4.6(36)
寫評論提問

说明书
  • YY1 抗体 H-10 是小鼠单克隆 IgG1 κ，YY1抗体, 在234篇文献中引用，规格为200 µg/ml
  • 免疫1-414氨基酸，对应于human来源YY1全长
  • YY1 抗体 (H-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 YY1
  • 抗YY1抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-7341 X, 200 µg/0.1 ml)
  • 关于如何获取YY1 (H-10): sc-7341的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 YY1 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与YY1 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 YY1 抗体包括 YY2

YY1抗体（H-10）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源YY1蛋白。YY1抗体（H-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。YY1单克隆抗体（H-10）可识别YY1转录因子，该因子在人类中也称为NF-E1，在小鼠中称为Delta或UCRBP。YY1 (H-10) 抗体针对一种在调节各种目标基因中发挥关键作用的蛋白质，因此 YY1 对于各种生物过程至关重要。YY1 位于细胞核中，与 DNA 相互作用以调节基因表达。四个 C 端锌指结构域和 N 端独特的结构特征增强了 YY1 作为转录调节因子的多功能性。YY1作为转录激活剂和转录抑制剂的双重功能凸显了其在维持细胞稳态和响应发育信号中的重要性。这种多方面的作用凸显了YY1在正常生理和疾病状态中的重要性，使YY1（H-10）单克隆抗体成为研究基因调控和转录动态的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

YY1 抗体 (H-10) 参考文献:

  1. 克隆与莫隆尼鼠白血病病毒上游保守区结合的负转录因子。  |  Flanagan, JR., et al. 1992. Mol Cell Biol. 12: 38-44. PMID: 1309593
  2. 人类 GLI-Krüppel 相关蛋白 YY1 的转录抑制作用以及腺病毒 E1A 蛋白对抑制作用的缓解。  |  Shi, Y., et al. 1991. Cell. 67: 377-88. PMID: 1655281
  3. 共同因子 1 是一种转录激活因子，可与 c-myc 启动子, 骨骼α-肌动蛋白启动子和免疫球蛋白重链增强子结合。  |  Riggs, KJ., et al. 1991. Mol Cell Biol. 11: 1765-9. PMID: 1899910
  4. Delta是一种转录因子，能与几种聚合酶II启动子中的下游元件结合，是一种功能多样的锌指蛋白。  |  Hariharan, N., et al. 1991. Proc Natl Acad Sci U S A. 88: 9799-803. PMID: 1946404
  5. 分离出一种可与免疫球蛋白 kappa 3' 增强子和免疫球蛋白重链 mu E1 位点结合的候选抑制因子/激活因子 NF-E1（YY-1，delta）。  |  Park, K. and Atchison, ML. 1991. Proc Natl Acad Sci U S A. 88: 9804-8. PMID: 1946405
  6. YY1 是增强子-启动子环路的结构调节器  |  Weintraub, AS., et al. 2017. Cell. 171: 1573-1588.e28. PMID: 29224777
  7. 组氨酸簇决定了相分离的增强子簇中的YY1-分区辅助激活子和染色质元素。  |  Wang, W., et al. 2022. Nucleic Acids Res. 50: 4917-4937. PMID: 35390165
  8. 小胶质细胞中的 YY1 乳化通过转录激活介导的 FGF2 上调促进血管生成。  |  Wang, X., et al. 2023. Genome Biol. 24: 87. PMID: 37085894
  9. M2巨噬细胞中的YY1复合物通过上调IL-6促进前列腺癌进展。  |  Chen, S., et al. 2023. J Immunother Cancer. 11: PMID: 37094986
  10. 肾YY1-KIM1-DR5轴调节急性肾损伤的进展。  |  Yang, C., et al. 2023. Nat Commun. 14: 4261. PMID: 37460623
  11. 转录因子 Sp1 和 YY1 之间的相互作用。  |  Seto, E., et al. 1993. Nature. 365: 462-4. PMID: 8003102
  12. 编码 delta/YY1/NF-E1/UCRBP 转录因子的小鼠基因的特征。  |  Sáfrány, G. and Perry, RP. 1993. Proc Natl Acad Sci U S A. 90: 5559-63. PMID: 8516301

订购信息

产品名称产品编号规格价格数量收藏夹

YY1 抗体 (H-10)

sc-7341
200 µg/ml
$316.00

YY1 (H-10): m-IgG Fc BP-HRP 套装

sc-528176
200 µg Ab; 10 µg BP
$354.00

YY1 (H-10): m-IgGκ BP-HRP 套装

sc-520493
200 µg Ab, 40 µg BP
$354.00

YY1 (H-10): m-IgG1 BP-HRP 套装

sc-542802
200 µg Ab; 20 µg BP
$354.00

YY1 抗体 (H-10) X

sc-7341 X
200 µg/0.1 ml
$316.00

YY1 抗体 (H-10) AC

sc-7341 AC
500 µg/ml, 25% agarose
$416.00

YY1 抗体 (H-10) HRP

sc-7341 HRP
200 µg/ml
$316.00

YY1 抗体 (H-10) FITC

sc-7341 FITC
200 µg/ml
$330.00

YY1 抗体 (H-10) PE

sc-7341 PE
200 µg/ml
$343.00

YY1 抗体 (H-10) Alexa Fluor® 488

sc-7341 AF488
200 µg/ml
$357.00

YY1 抗体 (H-10) Alexa Fluor® 546

sc-7341 AF546
200 µg/ml
$357.00

YY1 抗体 (H-10) Alexa Fluor® 594

sc-7341 AF594
200 µg/ml
$357.00

YY1 抗体 (H-10) Alexa Fluor® 647

sc-7341 AF647
200 µg/ml
$357.00

YY1 抗体 (H-10) Alexa Fluor® 680

sc-7341 AF680
200 µg/ml
$357.00

YY1 抗体 (H-10) Alexa Fluor® 790

sc-7341 AF790
200 µg/ml
$357.00

I would like to bulk order sc-7341 (H-10) YY1 antibody. Please advice how to proceed with the order. Thank you.

Asked by: yc227
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for a bulk quantity of this antibody. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Tech Service
Date published: 2019-12-02

Hi, We were interested in the sc-281X Anti-YY1 antibody. According to the catalog online, this has been discontinued and sc-7341 is now the recommended replacement. I would like to know if this is the suited alternative for ChIP expeiments. Thanks!

Asked by: Yudi
Thank you for your question. Yes, this mouse monoclonal, YY1 Antibody (H-10): sc-7341, has been published for use in ChIP research applications. Please use the product citation search on our website to search for specific publications by product, application and species. Or please give us a call in the Technical Service Department for support. https://www.scbt.com/scbt/misc/citationSearch.jsp
Answered by: Technical Support
Date published: 2019-06-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-7341 PE), fluorescein (sc-7341 FITC), Alexa Fluor® 488 (sc-7341 AF488) or Alexa Fluor® 647 (sc-7341 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_7341, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 81ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent specific signalI got a free sample of sc-7341, and tested WB on some breast cancer cell lines. It works very well and I strongly suggested it.
Date published: 2018-10-31
Rated 5 out of 5 by from good perfectgood antibody and magnificant results in our experiments
Date published: 2018-06-01
Rated 4 out of 5 by from Specific IHC-P Staining on Mouse TissueIHC-P staining was specific on mouse tissue while used in a somewhat high dilution (1/50). Lower dilution also showed okay but dimmer stain.
Date published: 2018-04-02
Rated 1 out of 5 by from ChIPWe have tested this antibody in ChIP with qPCR for known YY1 target sites in our cell model, but no enrichment was obtained. Antibodies for other transcription factors were used in the same experiment as positive controls, and they performed as expected.
Date published: 2018-02-09
Rated 5 out of 5 by from excellenttested on rat tissue samples and gave a very nice band.
Date published: 2017-12-13
Rated 5 out of 5 by from work well!Clear band can be observed in WB when CNEB cells were oerexpressed YY1.
Date published: 2017-11-28
Rated 5 out of 5 by from wonderful antibody!1:300 diluted for wb of protein extracted from h9c2 cells ,the band is strong and special!
Date published: 2017-11-01
Rated 5 out of 5 by from got the target bandWe got the target band 68kda very specific,I suggest 5% BSA in TBS blocking overnight at 4℃.No background seen.1:400 tried got strong signal,1:1000 also tried,but signal seems too weak.
Date published: 2017-09-16
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_36
  • loc_zh_CN, sid_7341, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
YY1 抗体 (H-10) is rated 4.6 out of 5 by 36.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_36
  • loc_zh_CN, sid_7341, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。