Date published: 2025-9-6

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XIAP 抗体 (E-2): sc-55551

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说明书
  • XIAP抗体(E-2)是小鼠单克隆IgG2b κ, 在44篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-202,在XIAP的human起源的N-terminus处进行映射
  • 抗-XIAP 抗体 (E-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 XIAP
  • 抗XIAP抗体(E-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取XIAP (E-2): sc-55551免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是XIAP Antibody (E-2) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与XIAP Antibody (E-2) 打包提供(请参阅下面的订购信息)。

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関連項目

XIAP 抗体(E-2)是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过西部印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附试验(ELISA)等应用检测小鼠、大鼠和人类样本中的 XIAP(X 染色体相关的细胞凋亡抑制因子)。抗 XIAP 抗体(E-2)有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 共轭物。XIAP(E-2)单克隆抗体可识别一种在调节细胞凋亡中起关键作用的蛋白质,尤其是通过抑制活化的 caspase-3,caspase-3 对细胞死亡的执行阶段至关重要。XIAP (E-2) 抗体靶向一种对细胞平衡和存活至关重要的蛋白质,尤其是在癌症中,逃避凋亡可使肿瘤细胞存活和增殖。抗 XIAP 抗体(E-2)可检测一种与凋亡抑制蛋白(IAP)家族其他成员(如存活素)相互作用的蛋白质,存活素参与细胞周期调控和有丝分裂过程中微管的稳定。XIAP 能够调节细胞凋亡途径,因此是旨在恢复癌细胞凋亡的治疗干预的重要靶点,从而提高了癌症治疗的效果。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

XIAP 抗体 (E-2) 参考文献:

  1. XIAP对细胞死亡和免疫的调节。  |  Jost, PJ. and Vucic, D. 2020. Cold Spring Harb Perspect Biol. 12: PMID: 31843992
  2. 靶向XIAP促进癌细胞死亡--ARTS和SMAC的故事。  |  Abbas, R. and Larisch, S. 2020. Cells. 9: PMID: 32182843
  3. 母体性性染色体嵌合导致XIAP缺失。  |  Tomomasa, D., et al. 2023. J Clin Immunol. 43: 525-527. PMID: 36441290
  4. 基于阿仑单抗的XIAP缺失减量治疗。  |  Kobushi, H., et al. 2023. Pediatr Int. 65: e15576. PMID: 37534620
  5. XIAP缺失中白细胞介素1介导的超炎症与自噬缺陷有关。  |  Dissanayake, D., et al. 2024. Blood. 144: 1183-1192. PMID: 38820590
  6. 杆状病毒 p35 蛋白可抑制 Fas 和肿瘤坏死因子诱导的细胞凋亡。  |  Beidler, DR., et al. 1995. J Biol Chem. 270: 16526-8. PMID: 7542648
  7. 杆状病毒抗凋亡蛋白 p35 对 ICE 家族蛋白酶的抑制。  |  Bump, NJ., et al. 1995. Science. 269: 1885-8. PMID: 7569933
  8. 表达杆状病毒 P35 可防止果蝇细胞死亡。  |  Hay, BA., et al. 1994. Development. 120: 2121-9. PMID: 7925015
  9. TNFR2-TRAF 信号复合体包含两个与杆状病毒凋亡抑制剂蛋白有关的新型蛋白。  |  Rothe, M., et al. 1995. Cell. 83: 1243-52. PMID: 8548810
  10. 克隆和表达具有抑制细胞凋亡和/或结合肿瘤坏死因子受体相关因子功能的细胞凋亡抑制蛋白同源物。  |  Uren, AG., et al. 1996. Proc Natl Acad Sci U S A. 93: 4974-8. PMID: 8643514
  11. 抵抗 Fas 介导的细胞凋亡:caspase 3 的激活受细胞周期调节因子 p21WAF1 和 IAP 基因家族 ILP 的调控。  |  Suzuki, A., et al. 1998. Oncogene. 17: 931-9. PMID: 9747872
  12. 存活素控制细胞凋亡和有丝分裂纺锤体检查点  |  Li, F., et al. 1998. Nature. 396: 580-4. PMID: 9859993

订购信息

产品名称产品编号规格价格数量收藏夹

XIAP 抗体 (E-2)

sc-55551
200 µg/ml
RMB2377.00

XIAP (E-2): m-IgG Fc BP-HRP 套装

sc-525637
200 µg Ab; 10 µg BP
RMB2662.00

XIAP (E-2): m-IgG2b BP-HRP 套装

sc-549602
200 µg Ab; 10 µg BP
RMB2662.00

XIAP 抗体 (E-2) AC

sc-55551 AC
500 µg/ml, 25% agarose
RMB3129.00

XIAP 抗体 (E-2) HRP

sc-55551 HRP
200 µg/ml
RMB2377.00

XIAP 抗体 (E-2) FITC

sc-55551 FITC
200 µg/ml
RMB2482.00

XIAP 抗体 (E-2) PE

sc-55551 PE
200 µg/ml
RMB2580.00

XIAP 抗体 (E-2) Alexa Fluor® 488

sc-55551 AF488
200 µg/ml
RMB2685.00

XIAP 抗体 (E-2) Alexa Fluor® 546

sc-55551 AF546
200 µg/ml
RMB2685.00

XIAP 抗体 (E-2) Alexa Fluor® 594

sc-55551 AF594
200 µg/ml
RMB2685.00

XIAP 抗体 (E-2) Alexa Fluor® 647

sc-55551 AF647
200 µg/ml
RMB2685.00

XIAP 抗体 (E-2) Alexa Fluor® 680

sc-55551 AF680
200 µg/ml
RMB2685.00

XIAP 抗体 (E-2) Alexa Fluor® 790

sc-55551 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for XIAP (E-2): sc-55551 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-55551.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-01-24
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Rated 5 out of 5 by from Very good in WBVery good ab with virtually no background for WB detection of endogenous human XIAP.
Date published: 2022-05-20
Rated 5 out of 5 by from 免疫印迹样品是乳腺癌细胞裂解液。做WB时,1;800-1000稀释,条带很漂亮,背景干净,与CST的XIAP抗体比较一下,相差无几。所以推荐购买,毕竟价格和量具有优势。
Date published: 2019-08-24
Rated 5 out of 5 by from Nice band in WBXIAP had strong band in WB in human prostate cancer cell line LNcap
Date published: 2018-07-09
Rated 5 out of 5 by from Strong band in WB in human breast cancer cell lineStrong band in WB in human breast cancer cell line t47d
Date published: 2018-02-01
Rated 4 out of 5 by from Work well!1:200 diluted for wb of rat protein ,the band is special,at the position of 50KD,but not quite strong
Date published: 2018-01-24
Rated 5 out of 5 by from 功能出色XIAP试用之后感觉还是不错,几分钟就发出来了,而且1:1000就可以,非常好用,下次还会回购,,对Santa满意
Date published: 2017-09-19
Rated 5 out of 5 by from OutstandingVery nice western blot data; strong signal, low background
Date published: 2017-02-04
Rated 5 out of 5 by from Extremely strong XIAP antibody!This antibody is really reliable and consistent in detecting XIAP in human and mouse samples. A great choice for WB, IP or IF experiments!
Date published: 2016-12-21
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XIAP 抗体 (E-2) is rated 4.9 out of 5 by 13.
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