Date published: 2025-9-8

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WTAP 抗体 (C-12): sc-166931

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说明书
  • WTAP抗体(C-12)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 免疫原氨基酸序列91-201位于human物种的WTAP的C-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的WTAP WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是WTAP Antibody (C-12) for WB applications. 的首选辅助检测试剂,这些试剂现与WTAP Antibody (C-12) 打包提供(请参阅下面的订购信息)。

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    関連項目

    威尔姆斯肿瘤(WT)是一种肾胚胎恶性肿瘤,每10000名婴儿中就有1名受其影响,它以散发性和遗传性两种形式出现。威尔姆斯肿瘤蛋白(WT1)与DNA序列GCGGGGGCG结合,这是Zn2+指转录激活因子的早期生长反应(Egr)家族共有的识别元素,并发挥转录阻遏物的作用。WTAP(威尔姆斯肿瘤1相关蛋白)是一种普遍表达的核蛋白,与WT1相互作用,可能参与调节mRNA剪接。WTAP位于核斑点中,通过与细胞周期蛋白A2的3'UTR结合来调节G2/M细胞周期转变,从而增强其稳定性。此外,WTAP抑制WT1靶基因的表达,并能够损害WT1与DNA结合的能力。由于选择性剪接事件的存在,WTAP有两种同种型。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    WTAP 抗体 (C-12) 参考文献:

    1. Wilms肿瘤蛋白WT1在器官发育中的作用。  |  Scholz, H. and Kirschner, KM. 2005. Physiology (Bethesda). 20: 54-9. PMID: 15653840
    2. Wilms肿瘤蛋白Wt1调控白细胞介素-10(IL-10)基因。  |  Sciesielski, LK., et al. 2010. FEBS Lett. 584: 4665-71. PMID: 20974136
    3. 针对血液系统恶性肿瘤和实体瘤患者,采用针对威尔姆斯肿瘤蛋白1(WT1)的主动特异性免疫疗法:早期临床试验的经验教训。  |  Van Driessche, A., et al. 2012. Oncologist. 17: 250-9. PMID: 22291091
    4. 威尔姆斯肿瘤蛋白(WT1)在发育中的人类外周交感神经和胃肠神经系统的免疫定位。  |  Parenti, R., et al. 2014. Acta Histochem. 116: 48-54. PMID: 23791475
    5. 威尔姆斯肿瘤蛋白在透明细胞肾细胞癌中诱导上皮-间质混合分化状态。  |  Sampson, VB., et al. 2014. PLoS One. 9: e102041. PMID: 25025131
    6. 威尔姆斯肿瘤蛋白识别特定DNA序列中的5-羧基胞嘧啶。  |  Hashimoto, H., et al. 2014. Genes Dev. 28: 2304-13. PMID: 25258363
    7. Wilms肿瘤蛋白Wt1通过调控输卵管蛋白稳态来促进女性生育。  |  Nathan, A., et al. 2017. Hum Mol Genet. 26: 1694-1705. PMID: 28334862
    8. 肾母细胞瘤蛋白1和脑肿瘤中5-甲基胞嘧啶的酶促氧化:潜在前景。  |  Ramsawhook, A., et al. 2018. Front Cell Dev Biol. 6: 26. PMID: 29623275
    9. 弥漫性桥脑胶质瘤中威尔姆斯肿瘤蛋白的差异表达。  |  Lee, S., et al. 2019. J Neuropathol Exp Neurol. 78: 380-388. PMID: 30990879
    10. 从健康人外周血单核细胞中体外扩增Wilms肿瘤蛋白1抗原表位特异性原代T细胞。  |  van der Heijden, S., et al. 2023. STAR Protoc. 4: 102053. PMID: 36853720

    订购信息

    产品名称产品编号规格价格数量收藏夹

    WTAP 抗体 (C-12)

    sc-166931
    200 µg/ml
    RMB2377.00

    WTAP (C-12): m-IgG Fc BP-HRP 套装

    sc-540177
    200 µg Ab; 10 µg BP
    RMB2662.00

    WTAP (C-12): m-IgG1 BP-HRP 套装

    sc-541951
    200 µg Ab; 20 µg BP
    RMB2662.00

    What is the recommended fixation method for immunofluorescence staining with WTAP (C-12): sc-166931 monoclonal antibody?

    Asked by: jerojero
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 1 out of 5 by from I tested the antibody on Western blot withI tested the antibody on Western blot with three different siRNAs against WTAP (verfied with qPCR - RNA levels were below 10%) and WTAP overexpression (wildtype and GFP tagged) in MCF7 cells. The antibody only recognized the overexpressed proteins but not the endogenous WTAP. However, the dominant band on the blot was unspecific at around 40kDa. I would not recommend this antibody for Western blot.
    Date published: 2015-06-15
    Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
    Date published: 2015-02-17
    Rated 5 out of 5 by from Nice Western Blot data of WTAP expressionNice Western Blot data of WTAP expression in Jurkat and K-562 nuclear extracts. -SCBT QC
    Date published: 2014-09-02
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    WTAP 抗体 (C-12) is rated 3.7 out of 5 by 3.
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