Date published: 2025-9-8

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Wnt-3 抗体 (D-9): sc-74537

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说明书
  • Wnt-3 抗体 D-9 是小鼠单克隆 IgG1 κ,Wnt-3抗体, 在23篇文献中引用,规格为200 µg/ml
  • 免疫human来源Wnt-3a近C-terminus的241-310氨基酸
  • Wnt-3 抗体 (D-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 precursor and mature Wnt-3 and Wnt-3a; 也和以下物种反应,包括: 和 bovine and porcine
  • 抗Wnt-3抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Wnt-3 (D-9): sc-74537免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Wnt-3 Antibody (D-9) for WB applications. 的首选辅助检测试剂,这些试剂现与Wnt-3 Antibody (D-9) 打包提供(请参阅下面的订购信息)。

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関連項目

Wnt-3 抗体(D-9)是一种 IgG1 κ 小鼠单克隆 Wnt-3 抗体(也称为 WNT3 抗体或 INT4 抗体),可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人源的 Wnt-3 蛋白质。Wnt-3 抗体(D-9)既可以是非偶联的抗 Wnt-3 抗体形式,也可以是多种偶联形式的抗 Wnt-3 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Wnt 基因家族编码分泌的信号分子,这些分子与卷曲受体结合并影响肿瘤发生和发育过程,包括胚胎发育过程中的细胞命运和模式的调控。根据其生物学活性,Wnt 家族具有两个功能类别:一类是通过稳定细胞质 β-连环蛋白的 Wnt-1/无翼途径发出信号的 Wnt,另一类是刺激细胞内 Ca2+ 释放并以 G 蛋白依赖的方式激活两种激酶 CamKII 和 PKC 的 Wnt。Wnt-3 在小脑发育过程中存在,并且在成人中仅限于浦肯野细胞层。在运动神经元中,Wnt-3 是一种逆行信号,控制肌肉传入的终端分支。人 Wnt-3 与小鼠 Wnt-3 蛋白质具有 98% 的同源性,与人 Wnt-3a 蛋白质具有 84% 的同源性。人 Wnt-3 基因与 Wnt-15 基因聚集在染色体 17q21 上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Wnt-3 抗体 (D-9) 参考文献:

  1. Wnt/Ca2+通路:一种新的脊椎动物Wnt信号通路正在形成。  |  Kühl, M., et al. 2000. Trends Genet. 16: 279-83. PMID: 10858654
  2. 由运动神经元表达的 WNT-3 可调节神经营养素-3 反应型脊髓感觉神经元的末端分枝。  |  Krylova, O., et al. 2002. Neuron. 35: 1043-56. PMID: 12354395
  3. 发育中小鼠前脑中 Wnt-3 基因的区域表达与间脑神经元的关系  |  Salinas, PC. and Nusse, R. 1992. Mech Dev. 39: 151-60. PMID: 1363370
  4. 高压氧治疗后缺氧缺血性新生大鼠中神经干细胞的增殖与Wnt-3蛋白相关。  |  Wang, XL., et al. 2007. Neuroreport. 18: 1753-6. PMID: 17921881
  5. Wnt-3a和Wnt-3通过典型信号传导,以不同的方式刺激脊髓神经前体的增殖和神经发生,并促进神经突起的生长。  |  David, MD., et al. 2010. J Neurosci Res. 88: 3011-23. PMID: 20722074
  6. Wnt-3是一种由小鼠乳腺肿瘤中的前病毒插入激活的基因,与int-1/Wnt-1同源,通常在小鼠胚胎和成脑中表达。  |  Roelink, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 4519-23. PMID: 2162045
  7. 鉴定 Wnt-3a 诱导人类神经干细胞早期分化所需的非经典 Wnt 受体  |  Bengoa-Vergniory, N., et al. 2017. Mol Neurobiol. 54: 6213-6224. PMID: 27709494
  8. 小鼠局灶性缺血性中风后鼻内注射 Wnt-3a 的神经保护和再生作用。  |  Wei, ZZ., et al. 2018. J Cereb Blood Flow Metab. 38: 404-421. PMID: 28430000
  9. Wnt-3a 通过调节小胶质细胞/巨噬细胞和星形胶质细胞的反应减轻缺血性中风后的神经炎症。  |  Zhang, D., et al. 2019. Int Immunopharmacol. 75: 105760. PMID: 31323530
  10. Wnt-3a 诱导人类牙髓干细胞的表观遗传重塑  |  Uribe-Etxebarria, V., et al. 2020. Cells. 9: PMID: 32156036
  11. Wnt3a 促进 SARS-CoV-2 伪病毒进入细胞  |  Melano, I., et al. 2023. Int J Mol Sci. 25: PMID: 38203386
  12. 小鼠小脑浦肯野细胞中 Wnt-3 表达的维持取决于与颗粒细胞的相互作用。  |  Salinas, PC., et al. 1994. Development. 120: 1277-86. PMID: 8026336

订购信息

产品名称产品编号规格价格数量收藏夹

Wnt-3 抗体 (D-9)

sc-74537
200 µg/ml
RMB2377.00

Wnt-3 (D-9): m-IgG Fc BP-HRP 套装

sc-527042
200 µg Ab; 10 µg BP
RMB2662.00

Wnt-3 (D-9): m-IgG1 BP-HRP 套装

sc-532415
200 µg Ab; 20 µg BP
RMB2662.00

Wnt-3 抗体 (D-9) AC

sc-74537 AC
500 µg/ml, 25% agarose
RMB3129.00

Wnt-3 抗体 (D-9) HRP

sc-74537 HRP
200 µg/ml
RMB2377.00

Wnt-3 抗体 (D-9) FITC

sc-74537 FITC
200 µg/ml
RMB2482.00

Wnt-3 抗体 (D-9) PE

sc-74537 PE
200 µg/ml
RMB2580.00

Wnt-3 抗体 (D-9) Alexa Fluor® 488

sc-74537 AF488
200 µg/ml
RMB2685.00

Wnt-3 抗体 (D-9) Alexa Fluor® 546

sc-74537 AF546
200 µg/ml
RMB2685.00

Wnt-3 抗体 (D-9) Alexa Fluor® 594

sc-74537 AF594
200 µg/ml
RMB2685.00

Wnt-3 抗体 (D-9) Alexa Fluor® 647

sc-74537 AF647
200 µg/ml
RMB2685.00

Wnt-3 抗体 (D-9) Alexa Fluor® 680

sc-74537 AF680
200 µg/ml
RMB2685.00

Wnt-3 抗体 (D-9) Alexa Fluor® 790

sc-74537 AF790
200 µg/ml
RMB2685.00

Are there any issues with using sc-74537: Wnt-3 (D-9) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-74537 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from GOODWorked well for western blotting at 1:500 ratio in breast cancer cell lines.
Date published: 2017-12-27
Rated 4 out of 5 by from CLEAN WESTERN BLOTThis antibody works very well in WB. (1:200 dilution)
Date published: 2017-12-21
Rated 4 out of 5 by from okDilution ratio in 1:100,detection signal is relatively strong, molecular weight :39kD.
Date published: 2017-10-10
Rated 5 out of 5 by from worked well !I have used the antibody in western blot, the band is very clear.
Date published: 2017-08-21
Rated 5 out of 5 by from GoodWorked well with little background and 1:500 dilution is fit for western.
Date published: 2017-05-16
Rated 5 out of 5 by from good featureWorked well for western blotting with murine tissue, specific and clear bands.
Date published: 2017-04-28
Rated 3 out of 5 by from Signal is relatively weak detected by WBSignal is relatively weak detected by WB at 1:100 ratio (Wnt-3: 5%BSA) in PC12 cells.
Date published: 2017-03-21
Rated 5 out of 5 by from Excellent choice for Wnt-3!This antibody is a great choice for Wnt-3 detection! Strong and reliable bands seen on Western blot.
Date published: 2017-01-23
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Wnt-3 抗体 (D-9) is rated 4.2 out of 5 by 11.
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