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WDR77 Antibody (FG-4) - Western Blotting - Image 134863
WDR77 抗体 (FG-4): sc-100899. SJRH30 (A), Sol8 (B), I-11. 15 (C) 和 F9 (D) 全细胞裂解液中 WDR77 表达的 Western 印迹分析.
WDR77 抗体 (FG-4): sc-100899. SJRH30 (A), Sol8 (B), I-11. 15 (C) 和 F9 (D) 全细胞裂解液中 WDR77 表达的 Western 印迹分析.

WDR77 抗体 (FG-4): sc-100899

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说明书
  • WDR77抗体(FG-4)是小鼠单克隆IgG1 κ, 在6篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组WDR77
  • 推荐用于 mouse, rat 和 human 来源的WDR77 WB, IP, IF, IHC(P) 和 ELISA检测
  • 目前，我们还没有完成WDR77 抗体 (FG-4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 WDR 抗体 包括 WDR1, WDR13, WDR20, WDR33, WDR4, WDR42A, WDR49, WDR5, WDR53和WDR55

WD重复序列是在多种蛋白质中发现的基序，其特征是由40-60个氨基酸组成的保守核心，通常形成三级螺旋桨结构。含有WD重复序列的蛋白质参与多种细胞功能，但它们通常涉及染色质组装、细胞周期控制、信号转导、RNA加工、细胞凋亡和囊泡运输等调控机制。WDR77（WD重复结构域77），也称为MEP50，是一种含有342个氨基酸的蛋白质，包含五个WD重复序列，被认为可以调节U snRNPs的早期组装。此外，WDR77作为含PRMT5的甲基转移酶复合物的组成部分，可将多种剪接体Sm蛋白中的精氨酸转化为二甲基精氨酸。这种转化随后将Sm蛋白靶向到运动神经元存活（SMN）复合物中，在那里它们被组装成核糖核蛋白核心颗粒。基于其与甲基转移酶复合物的关系，认为WDR77参与睾丸肿瘤的发展，提示其在致癌作用中的作用。

仅限研究使用。不适用于诊断和治疗用途。

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产品名称产品编号规格价格数量收藏夹

WDR77 抗体 (FG-4)

sc-100899
100 µg/ml
$333.00

你好！ 我已购买贵公司WDR77 (FG-4)抗体，货号sc-100899。想问下，该抗体是结合在抗原的C-末端还是N-末端，还是两端均可结合（full length）? 实验需要，期待您的尽快答复！ 谢谢！

Asked by: JingSha
感谢您的提问。 WDR77 Antibody (FG-4) 单克隆抗体免疫原是人源重组WDR77 的1-343氨基酸（accession # Q9BQA1）。Thank you for your question. WDR77 Antibody (FG-4) monoclonal antibody was raised against amino acids 1-343 of recombinant WDR77 of human origin (accession # Q9BQA1).
Answered by: Technical Support 8
Date published: 2018-12-21

Can sc-100899: WDR77 (FG-4) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, sc-100899: WDR77 (FG-4) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good imagesGood to use in western blot. The signal is pretty strong to detect.
Date published: 2021-10-04
Rated 5 out of 5 by from Excellent AntibodyThe WDR77 Antibody (FG-4): sc-100899 provides great staining data for IHC with formalin fixed, paraffin-embedded tissue sections showing cytoplasmic localization.
Date published: 2017-02-07
Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 whole cell lysateRobust signal in WB in NIH/3T3 whole cell lysate. -SCBT QC
Date published: 2015-06-08
Rated 5 out of 5 by from Good publishable data in WB in human 293TGood publishable data in WB in human 293T,H1299, H460, A549, LNCaP, MCF-7. -SCBT Publication Review
Date published: 2015-05-20
Rated 5 out of 5 by from Good signal strength in WB in mouse transfectedGood signal strength in WB in mouse transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Positive signal in WB in HeLa whole cell lysatePositive signal in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Strong band in WB in SJRH30Strong band in WB in SJRH30, Sol8, I-11.15 and F9 whole cell lysates.-SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-03-14
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WDR77 抗体 (FG-4) is rated 5.0 out of 5 by 9.
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