Date published: 2025-9-8
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von Willebrand Factor/VWF Antibody (F8/86) - Western Blotting - Image 16281
VWF 抗体 (F8/86): sc-53466. 人血小板提取物 (A) 和 HUV-EC-C 全细胞裂解液 (B) 中 VWF 表达的 Western 印迹分析.
von Willebrand Factor/VWF Antibody (F8/86) - Western Blotting - Image 16281
VWF 抗体 (F8/86): sc-53466. 人血小板提取物 (A) 和 HUV-EC-C 全细胞裂解液 (B) 中 VWF 表达的 Western 印迹分析.
von Willebrand Factor/VWF Antibody (F8/86) - Immunohistochemistry - Image 13938
VWF 抗体 (F8/86): sc-53466. 对福尔马林固定, 包埋的人扁桃体组织进行免疫过氧化物酶染色, 显示细胞质定位.
von Willebrand Factor/VWF Antibody (F8/86) - Immunohistochemistry - Image 353916
VWF 抗体 (F8/86): sc-53466. 福尔马林固定, 石蜡包埋的人脐带组织的免疫过氧化物酶染色, 显示脐静脉内皮细胞膜染色.
VWF 抗体 (F8/86): sc-53466. 人血小板提取物 (A) 和 HUV-EC-C 全细胞裂解液 (B) 中 VWF 表达的 Western 印迹分析.

von Willebrand Factor/VWF 抗体 (F8/86): sc-53466

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寫評論提問

说明书
  • von Willebrand Factor/VWF 抗体 F8/86 是小鼠单克隆 IgG1 κ，von Willebrand Factor/VWF抗体, 在31篇文献中引用，规格为200 µg/ml
  • 针对从human血浆中分离出的血管性血友病因子
  • von Willebrand Factor/VWF 抗体 (F8/86) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 VWF
  • 抗von Willebrand Factor/VWF抗体（F8/86）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取von Willebrand Factor/VWF (F8/86): sc-53466的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 von Willebrand Factor/VWF 抗体 (F8/86) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与von Willebrand Factor/VWF 抗体 (F8/86) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

VWF抗体（F8/86）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测血管性血友病因子（VWF）蛋白，在包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）在内的各种应用中具有极高的精确度。VWF是一种复杂的多聚糖蛋白，由多个重复单元组成，每个单元包含不同的结构域，如D'D3、A1、A2、A3和C末端区域，这些结构域对于其在止血中的作用至关重要。这种复杂的结构使VWF能够通过与血小板上的糖蛋白Ib（GPIb）受体相互作用，并与内皮下基质中的胶原结合，从而在血管损伤部位有效调节血小板的粘附和聚集。此外，VWF的多聚体性质增强了其作为凝血因子VIII载体的功能，而凝血因子VIII是凝血级联反应中至关重要的辅助因子，从而确保凝血因子VIII在凝块形成过程中的稳定性和可用性。通过翻译后修饰（如前肽切割和亚基间二硫键的形成）维持的VWF结构完整性对于其功能和防止过度出血的能力至关重要，因为VWF结构缺陷是血管性血友病（最常见的遗传性出血性疾病）的根本原因。抗VWF抗体（F8/86）是针对从人血浆中分离出的VWF而制备的，有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及多种Alexa Fluor®变体，以满足不同的实验需求。通过准确检测和分析VWF的结构域和多聚体形式，VWF单克隆抗体（F8/86）有助于深入研究血液凝固的分子机制、止血平衡的调节以及出血性疾病的病理生理学。无论是用于探索VWF结构动态的基础研究，还是用于评估患者样本中VWF水平和功能的临床诊断，VWF (F8/86)抗体都是研究血管生物学和制定治疗凝血相关疾病策略的有用工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

von Willebrand Factor/VWF 抗体 (F8/86) 参考文献:

  1. von Willebrand 因子多聚体和前肽裂解在结合和稳定因子 VIII 方面的作用。  |  Wise, RJ., et al. 1991. J Biol Chem. 266: 21948-55. PMID: 1939217
  2. 免疫组织学筛选在单克隆抗体生产中的价值。  |  Naiem, M., et al. 1982. J Immunol Methods. 50: 145-60. PMID: 6806388
  3. 人和重组 von Willebrand 因子的多聚化对血小板聚集, 与胶原蛋白结合以及与凝血因子 VIII 结合的影响。  |  Fischer, BE., et al. 1996. Thromb Res. 84: 55-66. PMID: 8885147
  4. 冯-威廉因子 A1 结构域与组蛋白的结合。  |  Ward, CM., et al. 1997. Thromb Res. 86: 469-77. PMID: 9219327
  5. 人类冯-维勒布兰德因子 A 型结构域配体和突变位点的分子建模及其与冯-维勒布兰德氏病的相关性。  |  Jenkins, PV., et al. 1998. Blood. 91: 2032-44. PMID: 9490688
  6. von Willebrand 病的分子遗传学。  |  Mazurier, C., et al. 1998. Ann Genet. 41: 34-43. PMID: 9599650
  7. 因子 VIII 与 von Willebrand 因子的结合可通过裂解 von Willebrand 因子的前肽来实现，并通过形成二硫键连接的多聚体来增强。  |  Bendetowicz, AV., et al. 1998. Blood. 92: 529-38. PMID: 9657753

订购信息

产品名称产品编号规格价格数量收藏夹

von Willebrand Factor/VWF 抗体 (F8/86)

sc-53466
200 µg/ml
RMB2377.00

von Willebrand Factor/VWF (F8/86): m-IgG Fc BP-HRP 套装

sc-528569
200 µg Ab; 10 µg BP
RMB2662.00

von Willebrand Factor/VWF (F8/86): m-IgGκ BP-HRP 套装

sc-520980
200 µg Ab, 40 µg BP
RMB2662.00

von Willebrand Factor/VWF (F8/86): m-IgG1 BP-HRP 套装

sc-543001
200 µg Ab; 20 µg BP
RMB2662.00

von Willebrand Factor/VWF 抗体 (F8/86) AC

sc-53466 AC
500 µg/ml, 25% agarose
RMB3129.00

von Willebrand Factor/VWF 抗体 (F8/86) HRP

sc-53466 HRP
200 µg/ml
RMB2377.00

von Willebrand Factor/VWF 抗体 (F8/86) FITC

sc-53466 FITC
200 µg/ml
RMB2482.00

von Willebrand Factor/VWF 抗体 (F8/86) PE

sc-53466 PE
200 µg/ml
RMB2580.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 488

sc-53466 AF488
200 µg/ml
RMB2685.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 546

sc-53466 AF546
200 µg/ml
RMB2685.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 594

sc-53466 AF594
200 µg/ml
RMB2685.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 647

sc-53466 AF647
200 µg/ml
RMB2685.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 680

sc-53466 AF680
200 µg/ml
RMB2685.00

von Willebrand Factor/VWF 抗体 (F8/86) Alexa Fluor® 790

sc-53466 AF790
200 µg/ml
RMB2685.00

Does Mab F8/86 react with pig?

Asked by: rbc3
Thank you for your question. We have not validated the antibody to work with pig . There is a 74% homology in pig and we only recommend if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2024-03-28

Hi, Please can you tell me if you have a VWF antibody suitable for a construct of a region in the A2 domain (aa 1596-1668) for use in western blot? BW, Zoe

Asked by: zoeml
Thank you for your question. We do not offer a VWF antibody with an epitope range of 1596-1668.
Answered by: BlakeJ
Date published: 2023-02-23

Is this antibody able to stain human frozen sections?　If so, please let me know the recommended concentration.

Asked by: wakamore
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended antigen retrieval method when working with paraffin-embedded sections in IHC?

Asked by: TinTin
Thank you for your question. We recommend using sodium citrate buffer, pH 6, with heat for antigen retrieval. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-02-03
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Rated 1 out of 5 by from no signalIHC. No signal with PFA or glyoxal fixed brain slices.
Date published: 2020-05-29
Rated 5 out of 5 by from VWF Antibody WBThis antibody works well on Western Blot. We obtained predicted size band on platelet extracts with minimal background.
Date published: 2018-02-27
Rated 5 out of 5 by from excellentVWF antibody was diluted at WB in 1:500 and the band was clear. I satisfied to the utmost degree the result.
Date published: 2017-10-26
Rated 1 out of 5 by from Did not work for flow cytometryPart of an experiment testing multiple vWF antibodies for IHC on primary lung cells. Did not work, but it could be due to the antigen being intracellular. No permeabilization was performed.
Date published: 2017-09-20
Rated 5 out of 5 by from good data of WB and IFI obtained clear bands in Western Blot use dilution 1;800, also performs well in IF staining.
Date published: 2017-06-03
Rated 5 out of 5 by from Good antibodyGood quality antibody for WB with gave great results in detect human platelet extract
Date published: 2017-05-20
Rated 5 out of 5 by from great for wbThis VWF antibody is very good,for wb dilution at 1:500,It showed very good perfomance
Date published: 2017-02-12
Rated 5 out of 5 by from Good VWF detectionPublished antibody that produced good results in WB and IHC.
Date published: 2017-02-07
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von Willebrand Factor/VWF 抗体 (F8/86) is rated 4.4 out of 5 by 13.
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