Date published: 2025-9-8
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VR1 Antibody (E-8) - Western Blotting - Image 376085
VR1 抗体 (E-8): sc-398417. F9 (A), TK-1 (B), WEHI-231 (C) 和 C6 (D) 全细胞裂解液中 VR1 表达的 Western 印迹分析.
VR1 Antibody (E-8) - Western Blotting - Image 376085
VR1 抗体 (E-8): sc-398417. F9 (A), TK-1 (B), WEHI-231 (C) 和 C6 (D) 全细胞裂解液中 VR1 表达的 Western 印迹分析.
VR1 Antibody (E-8) - Western Blotting - Image 375286
VR1 抗体 (E-8): sc-398417. F9 全细胞裂解液中 VR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.
VR1 Antibody (E-8) - Western Blotting - Image 304485
VR1 抗体 (E-8): sc-398417. 非转染: sc-110760 (A) 和小鼠 VR1 转染: sc-179742 (B) 293 全细胞裂解液中 VR1 表达的 Western 印迹分析.
VR1 抗体 (E-8): sc-398417. F9 (A), TK-1 (B), WEHI-231 (C) 和 C6 (D) 全细胞裂解液中 VR1 表达的 Western 印迹分析.

VR1 抗体 (E-8): sc-398417

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说明书
  • VR1 抗体 E-8 是小鼠单克隆 IgG2a κ，VR1抗体, 在21篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-130位于rat物种的VR1的N-terminus
  • VR1 抗体 (E-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse 和rat 来源的 VR1
  • 抗VR1抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VR1 (E-8): sc-398417的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是VR1 Antibody (E-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VR1 Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外VR抗体，包括VRL-1

VR1抗体（E-8）是一种IgG2a κ型小鼠单克隆VR1抗体（也称为Trpv1抗体），可通过WB、IP、IF和ELISA检测小鼠和大鼠来源的VR1蛋白。VR1抗体（E-8）既有非偶联抗VR1抗体形式，也有多种偶联形式的抗VR1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。香草醛受体1（VR1），也被称为辣椒素受体，是一种非选择性阳离子通道，在结构上与TRP离子通道家族成员相关。VR1可被辣椒中的活性成分辣椒素、热量以及在感染、炎症和缺血部位质子的增加而激活。通过创造适度的酸性条件，质子能够降低VR1激活的温度阈值，从而将VR1确定为引发疼痛的化学和物理刺激的分子整合器。VR1在初级感觉神经元和迷走神经中表达，激活的VR1诱导阳离子，特别是Ca2+和Na+离子的流入。除了神经系统和心血管系统中的大麻素受体外，香草醛受体还可能是内源性花生四烯酸乙醇胺的分子靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VR1 抗体 (E-8) 参考文献:

  1. 受热门控的离子通道  |  Cesare, P., et al. 1999. Proc Natl Acad Sci U S A. 96: 7658-63. PMID: 10393876
  2. 感觉神经上的类香兰素受体介导了安乃近的血管扩张作用。  |  Zygmunt, PM., et al. 1999. Nature. 400: 452-7. PMID: 10440374
  3. 辣椒素和 VR1 受体的外周和中枢作用。  |  Sasamura, T. and Kuraishi, Y. 1999. Jpn J Pharmacol. 80: 275-80. PMID: 10496326
  4. 外周神经损伤对功能性脊髓VR1受体的影响。  |  Kelly, S. and Chapman, V. 2002. Neuroreport. 13: 1147-50. PMID: 12151758
  5. Ca2+/钙调蛋白依赖性激酶 II 对香草素受体 1 的磷酸化调节其香草素结合。  |  Jung, J., et al. 2004. J Biol Chem. 279: 7048-54. PMID: 14630912
  6. 利用荧光成像平板阅读器（FLIPR）测定小鼠冷薄荷醇受体 TRPM8 和类香草素受体 1 型 VR1 的特性。  |  Behrendt, HJ., et al. 2004. Br J Pharmacol. 141: 737-45. PMID: 14757700
  7. 茸毛受体 1 可调节多个钙离子区，并有助于感觉神经元中 Ca2+ 诱导的 Ca2+ 释放。  |  Kárai, LJ., et al. 2004. J Biol Chem. 279: 16377-87. PMID: 14963041
  8. 调节外泌有助于蛋白激酶 C 强化香草醛受体的活性。  |  Morenilla-Palao, C., et al. 2004. J Biol Chem. 279: 25665-72. PMID: 15066994
  9. 嘌呤能和类香草素受体激活会从孤束核的不同颅传入终端释放谷氨酸。  |  Jin, YH., et al. 2004. J Neurosci. 24: 4709-17. PMID: 15152030
  10. 4-(3-三氟甲基吡啶-2-基)哌嗪-1-羧酸（5-三氟甲基吡啶-2-基）酰胺--一种高亲和力 TRPV1（VR1）类香草素受体拮抗剂的鉴定和生物学评价。  |  Swanson, DM., et al. 2005. J Med Chem. 48: 1857-72. PMID: 15771431
  11. 辣椒素受体：疼痛通路中的热激活离子通道。  |  Caterina, MJ., et al. 1997. Nature. 389: 816-24. PMID: 9349813
  12. 克隆的辣椒素受体整合了多种产生疼痛的刺激。  |  Tominaga, M., et al. 1998. Neuron. 21: 531-43. PMID: 9768840

订购信息

产品名称产品编号规格价格数量收藏夹

VR1 抗体 (E-8)

sc-398417
200 µg/ml
RMB2377.00

VR1 (E-8): m-IgG Fc BP-HRP 套装

sc-530787
200 µg Ab; 10 µg BP
RMB2662.00

VR1 (E-8): m-IgGκ BP-HRP 套装

sc-524329
200 µg Ab, 40 µg BP
RMB2662.00

VR1 (E-8): m-IgG2a BP-HRP 套装

sc-547589
200 µg Ab; 10 µg BP
RMB2662.00

VR1 抗体 (E-8) AC

sc-398417 AC
500 µg/ml, 25% agarose
RMB3129.00

VR1 抗体 (E-8) HRP

sc-398417 HRP
200 µg/ml
RMB2377.00

VR1 抗体 (E-8) FITC

sc-398417 FITC
200 µg/ml
RMB2482.00

VR1 抗体 (E-8) PE

sc-398417 PE
200 µg/ml
RMB2580.00

VR1 抗体 (E-8) Alexa Fluor® 488

sc-398417 AF488
200 µg/ml
RMB2685.00

VR1 抗体 (E-8) Alexa Fluor® 546

sc-398417 AF546
200 µg/ml
RMB2685.00

VR1 抗体 (E-8) Alexa Fluor® 594

sc-398417 AF594
200 µg/ml
RMB2685.00

VR1 抗体 (E-8) Alexa Fluor® 647

sc-398417 AF647
200 µg/ml
RMB2685.00

VR1 抗体 (E-8) Alexa Fluor® 680

sc-398417 AF680
200 µg/ml
RMB2685.00

VR1 抗体 (E-8) Alexa Fluor® 790

sc-398417 AF790
200 µg/ml
RMB2685.00

请问说明书中的阳性对照293 Lysate指的是HeK293细胞的溶解产物吗？

Asked by: WYX111
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

The gel shown for the VR1 Antibody (E-8) sc-398417 is gradient gel or other? Please, provide the composition of the Gel. Thank you

Asked by: Pawas Guru
Thank you for your question. The images for sc-398417 are of a 10% SDS-PAGE polyacrylamide gel.
Answered by: Tech Service
Date published: 2019-10-09

Hello, I was wondering if I could get avail of the free sample of VR1 (E-8): sc-398417 conjugated to AlexaFlour 488 for Flow Cytometry? Many Thanks, Aisling

Asked by: Aisling
Thank you for your question. We do not provide sample sizes for our conjugated primary antibodies. The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM).
Answered by: Technical Support
Date published: 2019-01-26

Can this antibody be used with the immunoflourscence of formalin fixed tissues ?? 

Asked by: Sara91
Thank you for your question. VR1 Antibody (E-8): sc-398417 is recommended for detection of VR1 of mouse and rat origin by WB, IP, IF and ELISA. It is not recommended for IHC or IHC(P).
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Could this Ab use in Mouse DRG fluorescent immunostaining?

Asked by: Aurora K
Thank you for your question. This antibody is mouse reactive and should be fine for detection in DRG neurons, as long as VR1 is expressed in your DRG cells. If you have any further questions, please reach out to us at [email protected], or by phone or live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 5 out of 5 by from Clear and Specific ResultsGood antibodies work at WB diluted with 1:200, it's clear that the molecular weight in the right single band is in line with my requirements.
Date published: 2017-11-02
Rated 4 out of 5 by from Great features!Western blots were performed to detect the protein expression of human papillary thyroid cancer BCPAP cells. Cell lysates were extracted by RIPA buffer containing protease inhibitor cocktail. 50 µg of total protein was separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The proteins were electroblotted onto nitrocellulose (NC) membrane. The membranes were probed with the TRPV1 primary antibodies (sc-398417), followed by incubation with horseradish peroxidase conjugated secondary antibody. We got a light band at around 100 kDa, and a dark band at around 50 kDa. So i wonder if this product is able to correspond to human TRPV1.
Date published: 2017-10-22
Rated 5 out of 5 by from Strong singalI have tested this antibody on mice brain lysates . Diluted the antibody at 1:1000 use 5% BSA in TBST.Strong singal at 100kda.
Date published: 2017-08-12
Rated 5 out of 5 by from working very goodThe antibody working very good, giving clear strong signal in primary mouse brain endothelial cells at indicated dilution 1:500.
Date published: 2017-07-23
Rated 5 out of 5 by from A very good antibodyA very good antibody, diluted 1:500 products performed well. I will continue to use this product.
Date published: 2017-06-06
Rated 5 out of 5 by from Antibody effect is very goodWe use this antibody WB, it is always good, we will continue to use.
Date published: 2017-06-02
Rated 5 out of 5 by from high quality for WBI am so happy that I bought this antibody.I compared it with other supplier`s antibodies,and this antibody is more specific and high quality.
Date published: 2017-05-28
Rated 5 out of 5 by from Will continue to buy antibodiesPowerful function of antibody, dilute 1:500 product performance is good. Our company will continue to buy the antibody
Date published: 2017-02-09
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VR1 抗体 (E-8) is rated 4.9 out of 5 by 12.
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