Date published: 2025-9-7

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VPS35 抗体 (B-5): sc-374372

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说明书
  • VPS35 抗体 B-5 是小鼠单克隆 IgG2b κ,VPS35抗体, 在41篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列497-796位于human物种的VPS35的C-terminus
  • VPS35 抗体 (B-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 VPS35; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗VPS35抗体(B-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VPS35 (B-5): sc-374372免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是VPS35 Antibody (B-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与VPS35 Antibody (B-5) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

VPS35抗体(B-5)是一种IgG2b κ型小鼠单克隆VPS35抗体,可通过WB、IP、IF、IHC(P)和ELISA技术检测小鼠、大鼠和人类来源的VPS35蛋白。VPS35抗体(B-5)既有非偶联的抗VPS35抗体形式,也有多种偶联形式的抗VPS35抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。液泡分选蛋白(VPSs)对于内吞和生物合成蛋白正确转运到液泡中是必需的,并在细胞的出芽过程中发挥重要作用。VPS35(液泡蛋白分选35),也称为MEM3,是酿酒酵母Vps35蛋白的796个氨基酸的人类同源物。VPS35定位于细胞质和周边膜,是retromer复合物的必需组分,该复合物参与从内体到反式高尔基网的溶酶体酶受体的回收。VPS35在心脏、胎盘、脑、睾丸、肾脏、结肠、卵巢和脾脏中表达最高,呈泛在性表达。除了其在retromer复合物中的关键作用外,VPS35还是一个亚复合物的组成部分,该亚复合物对于调节聚合免疫球蛋白受体从上皮细胞和肝细胞的基底外侧到顶端表面的转胞吞作用是必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VPS35 抗体 (B-5) 参考文献:

  1. 酵母空泡蛋白分选 29 和 35 的人类同源物。  |  Edgar, AJ. and Polak, JM. 2000. Biochem Biophys Res Commun. 277: 622-30. PMID: 11062004
  2. 酵母液泡蛋白分选蛋白 Vps26, 29 和 35 的人类直向同源物:组装成多聚体复合物。  |  Haft, CR., et al. 2000. Mol Biol Cell. 11: 4105-16. PMID: 11102511
  3. 人类 VPS35 和小鼠 Vps35 的克隆和表征以及 VPS35 在人类染色体 16q13-q21 上的映射。  |  Zhang, P., et al. 2000. Genomics. 70: 253-7. PMID: 11112353
  4. 哺乳动物的 retromer 可调节聚合免疫球蛋白受体的转囊作用。  |  Vergés, M., et al. 2004. Nat Cell Biol. 6: 763-9. PMID: 15247922
  5. retromer 亚基 Vps26 具有捕获素折叠,并通过其 C 端结构域与 Vps35 结合。  |  Shi, H., et al. 2006. Nat Struct Mol Biol. 13: 540-8. PMID: 16732284
  6. 人类 Vps29 retromer 成分是一种阳离子依赖性 6-磷酸甘露糖受体底物肽的金属-磷酸酯酶。  |  Damen, E., et al. 2006. Biochem J. 398: 399-409. PMID: 16737443
  7. 液泡-ATP 酶抑制剂巴佛洛霉素和突变体 VPS35 可抑制典型的 Wnt 信号传导。  |  George, A., et al. 2007. Neurobiol Dis. 26: 125-33. PMID: 17239604
  8. retromer 货物识别复合体的功能结构。  |  Hierro, A., et al. 2007. Nature. 449: 1063-7. PMID: 17891154
  9. 针对液泡缺陷的新型免疫检测筛选确定了麦角菌中 VPS35 的独特等位基因。  |  Takahashi, MK., et al. 2008. Mol Cell Biochem. 311: 121-36. PMID: 18224426
  10. VPS35 在帕金森病病理生物学中的作用。  |  Sassone, J., et al. 2021. Cell Mol Neurobiol. 41: 199-227. PMID: 32323152
  11. VPS35 通过表皮生长因子受体再循环促进细胞增殖,并增强胃癌对表皮生长因子受体抑制剂的反应。  |  Yu, J., et al. 2023. EBioMedicine. 89: 104451. PMID: 36738481
  12. VPS35 通过整合素/FAK/SRC 信号介导的 IL-6/STAT3 通路激活,以 YAP 依赖性方式促进胃癌进展。  |  Zhou, Q., et al. 2024. Oncogene. 43: 106-122. PMID: 37950040

订购信息

产品名称产品编号规格价格数量收藏夹

VPS35 抗体 (B-5)

sc-374372
200 µg/ml
RMB2377.00

VPS35 (B-5): m-IgGκ BP-HRP 套装

sc-522715
200 µg Ab, 40 µg BP
RMB2662.00

VPS35 抗体 (B-5) AC

sc-374372 AC
500 µg/ml, 25% agarose
RMB3129.00

VPS35 抗体 (B-5) HRP

sc-374372 HRP
200 µg/ml
RMB2377.00

VPS35 抗体 (B-5) FITC

sc-374372 FITC
200 µg/ml
RMB2482.00

VPS35 抗体 (B-5) PE

sc-374372 PE
200 µg/ml
RMB2580.00

VPS35 抗体 (B-5) Alexa Fluor® 488

sc-374372 AF488
200 µg/ml
RMB2685.00

VPS35 抗体 (B-5) Alexa Fluor® 546

sc-374372 AF546
200 µg/ml
RMB2685.00

VPS35 抗体 (B-5) Alexa Fluor® 594

sc-374372 AF594
200 µg/ml
RMB2685.00

VPS35 抗体 (B-5) Alexa Fluor® 647

sc-374372 AF647
200 µg/ml
RMB2685.00

VPS35 抗体 (B-5) Alexa Fluor® 680

sc-374372 AF680
200 µg/ml
RMB2685.00

VPS35 抗体 (B-5) Alexa Fluor® 790

sc-374372 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to use with VPS35 Antibody (B-5): sc-374372?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from Excellent Antibody for VPS35A clear and reliable antibody to detect endogenous VPS35 from both mouse and human samples.
Date published: 2023-10-24
Rated 5 out of 5 by from Excellent antibody for Western BlotI tested this antibody on mouse embryonic stem cells (mESCs) and it worked very well by western blot (dilution 1:1000, over-night incubation, blocking in milk).
Date published: 2022-03-02
Rated 5 out of 5 by from Works very well for WBSingle immunoreactive band at the expected MW on lysates of Jurkat T cells.
Date published: 2019-01-22
Rated 5 out of 5 by from Works very well!I tested this antibody by western blot and ICC on human iPSC derived neurons and it works very well !
Date published: 2018-06-25
Rated 5 out of 5 by from very good!It's work well at the dilotion of 1:1000 in western blot.The band is quite clear.
Date published: 2018-04-04
Rated 5 out of 5 by from VPS35 Antikörper (B-5)Works really good in IF, 1:100 dilution. Nice Colocalization with other retromer components. I have tested so far only human lung fibroblasts.
Date published: 2018-01-23
Rated 5 out of 5 by from Good western blotting antibodyexcellent single band from human cell lysates at 1/1000
Date published: 2017-09-20
Rated 5 out of 5 by from Excellent antibodyN2A total sample was probed with this antibody (1:1000). Thermo regularly strength solution was used for detection.
Date published: 2017-08-11
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VPS35 抗体 (B-5) is rated 5.0 out of 5 by 18.
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