Date published: 2025-9-8

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VMAT 2 抗体 (H-12): sc-374079

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说明书
  • VMAT 2 抗体 H-12 是小鼠单克隆 IgG1 κ,VMAT 2抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列44-133位于human物种的VMAT 2的N-terminus附近
  • VMAT 2 抗体 (H-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 VMAT 2
  • 抗VMAT 2抗体(H-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VMAT 2 (H-12): sc-374079免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 VMAT 2 抗体 (H-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与VMAT 2 抗体 (H-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

VMAT 2抗体(H-12)是一种IgG1 κ小鼠单克隆VMAT 2抗体(也称为VMAT2抗体、溶质载体家族18成员A2抗体、SVMT抗体、突触囊泡胺转运蛋白抗体、囊泡胺转运蛋白2抗体、SVAT抗体、VAT2抗体、单胺类神经递质转运蛋白抗体、溶质载体家族18成员2抗体、囊泡单胺/ H+反转运蛋白抗体、单胺转运蛋白抗体或PKDYS2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的VMAT 2蛋白。VMAT 2抗体(H-12)既可用作非偶联形式的抗VMAT 2抗体,也可用作多种偶联形式的抗VMAT 2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。神经传递依赖于化学递质分子的调节性胞吐释放。这需要将这些物质包装到神经元和神经内分泌细胞的特殊分泌囊泡中,这一过程由特定的囊泡转运蛋白介导。编码单胺类(VMAT 1和VMAT 2)和乙酰胆碱(VACht)囊泡转运蛋白的基因家族已被克隆并进行了功能鉴定。这些整体膜蛋白的序列预测有十二个跨膜结构域,与一类细菌抗生素抗性蛋白有微弱的同源性。神经递质分子的囊泡转运已被证明是一种依赖ATP和质子的主动转运机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VMAT 2 抗体 (H-12) 参考文献:

  1. 治疗亨廷顿病的新一代VMAT-2抑制剂NBI-641449。  |  Chen, S., et al. 2015. CNS Neurosci Ther. 21: 662-71. PMID: 26122704
  2. 治疗迟发性运动障碍的新一代VMAT-2抑制剂。  |  Correll, CU. and Carbon, M. 2017. Lancet Psychiatry. 4: 574-575. PMID: 28668670
  3. 用VMAT-2抑制剂治疗迟发性运动障碍:随机对照试验的系统评价和荟萃分析。  |  Solmi, M., et al. 2018. Drug Des Devel Ther. 12: 1215-1238. PMID: 29795977
  4. 囊泡单胺转运体(VMAT-2)抑制剂在精神分裂症和精神病中的荟萃分析和系统综述。  |  Connolly, A., et al. 2024. Psychopharmacology (Berl). 241: 225-241. PMID: 38238580
  5. VMAT-2抑制剂的传播:治疗迟发性运动障碍和亨廷顿病的新一代药物。  |  Ma, E., et al. 2025. Neurol Clin Pract. 15: e200392. PMID: 39399570
  6. 绒毛膜颗粒中的囊泡单胺转运体的生物化学和分子生物学。  |  Henry, JP., et al. 1994. J Exp Biol. 196: 251-62. PMID: 7823026
  7. 推测的乙酰胆碱囊泡转运体的分子克隆。  |  Roghani, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 10620-4. PMID: 7938002
  8. 大鼠大脑突触小泡的乙酰胆碱主动转运。  |  Haigh, JR., et al. 1994. Neuroreport. 5: 773-6. PMID: 8018848
  9. 囊泡单胺转运体的药理特征与多药转运体相似。  |  Yelin, R. and Schuldiner, S. 1995. FEBS Lett. 377: 201-7. PMID: 8543051
  10. 人类囊泡乙酰胆碱转运体对乙酰胆碱的主动转运。  |  Varoqui, H. and Erickson, JD. 1996. J Biol Chem. 271: 27229-32. PMID: 8910293
  11. 囊泡神经递质转运体。调节突触功能的潜在场所。  |  Varoqui, H. and Erickson, JD. 1997. Mol Neurobiol. 15: 165-91. PMID: 9396009
  12. 囊泡神经递质转运和突触前对量子大小的调节  |  Reimer, RJ., et al. 1998. Curr Opin Neurobiol. 8: 405-12. PMID: 9687352

订购信息

产品名称产品编号规格价格数量收藏夹

VMAT 2 抗体 (H-12)

sc-374079
200 µg/ml
RMB2377.00

VMAT 2 (H-12): m-IgG Fc BP-HRP 套装

sc-529653
200 µg Ab; 10 µg BP
RMB2662.00

VMAT 2 (H-12): m-IgGκ BP-HRP 套装

sc-522611
200 µg Ab, 40 µg BP
RMB2662.00

VMAT 2 抗体 (H-12) AC

sc-374079 AC
500 µg/ml, 25% agarose
RMB3129.00

VMAT 2 抗体 (H-12) HRP

sc-374079 HRP
200 µg/ml
RMB2377.00

VMAT 2 抗体 (H-12) FITC

sc-374079 FITC
200 µg/ml
RMB2482.00

VMAT 2 抗体 (H-12) PE

sc-374079 PE
200 µg/ml
RMB2580.00

VMAT 2 抗体 (H-12) Alexa Fluor® 488

sc-374079 AF488
200 µg/ml
RMB2685.00

VMAT 2 抗体 (H-12) Alexa Fluor® 546

sc-374079 AF546
200 µg/ml
RMB2685.00

VMAT 2 抗体 (H-12) Alexa Fluor® 594

sc-374079 AF594
200 µg/ml
RMB2685.00

VMAT 2 抗体 (H-12) Alexa Fluor® 647

sc-374079 AF647
200 µg/ml
RMB2685.00

VMAT 2 抗体 (H-12) Alexa Fluor® 680

sc-374079 AF680
200 µg/ml
RMB2685.00

VMAT 2 抗体 (H-12) Alexa Fluor® 790

sc-374079 AF790
200 µg/ml
RMB2685.00

Can VMAT 2 (H-12): sc-374079 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, VMAT 2 (H-12): sc-374079 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great antibody!!I used this antibody for western blot and immunofluorescence with rat samples. It worked properly with a clear single band per lane (WB) and a peripheral pattern of expression in PC12 cells (IF).
Date published: 2020-05-17
Rated 5 out of 5 by from WorksUsing mouse brain striatal tissue, 1:1000 dilution, I could see a clear band using western blotting.
Date published: 2018-04-03
Rated 5 out of 5 by from EasinessI used the VMAT2 at 1:300 in Western Blot, PC12 cells. It's easy to obtain the protein bands which are clear. There are not other protein bands.
Date published: 2017-09-18
Rated 5 out of 5 by from Produced positive Western Blot data of VMATProduced positive Western Blot data of VMAT 2 expression in IMR-32, SK-N-MC, Jurkat, JAR, SH-SY5Y and K-562 whole cell lysates. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Produced positive Western Blot data of VMATProduced positive Western Blot data of VMAT 2 expression in IMR-32 and SK-N-MC whole cell lysates. -SCBT QC
Date published: 2013-04-22
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VMAT 2 抗体 (H-12) is rated 5.0 out of 5 by 5.
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