Date published: 2025-9-8
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VMAT 1 Antibody (G-12) - Western Blotting - Image 372962
VMAT 1 抗体 (G-12): sc-166391. Caki-1 (A), Jurkat (B), RAW 264. 7 (C) 和 PC-12 (D) 全细胞裂解液中 VMAT 1 表达的 Western 印迹分析.
VMAT 1 抗体 (G-12): sc-166391. Caki-1 (A), Jurkat (B), RAW 264. 7 (C) 和 PC-12 (D) 全细胞裂解液中 VMAT 1 表达的 Western 印迹分析.

VMAT 1 抗体 (G-12): sc-166391

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寫評論提問

说明书
  • VMAT 1 抗体 G-12 是小鼠单克隆 IgG2b κ，VMAT 1抗体, 在2篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列44-143位于human物种的VMAT 1的N-terminus附近
  • VMAT 1 抗体 (G-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 VMAT 1
  • 抗VMAT 1抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取VMAT 1 (G-12): sc-166391的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是VMAT 1 Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与VMAT 1 Antibody (G-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外VMAT抗体，包括VMAT 2

VMAT 1抗体（G-12）是一种IgG2b κ小鼠单克隆VMAT 1抗体（也被称为SLC18A1抗体、VMAT1抗体或VAT1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的VMAT 1蛋白。VMAT 1抗体（G-12）既有非偶联抗VMAT 1抗体形式，也有多种偶联形式的抗VMAT 1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。神经传递依赖于化学递质分子的调节性胞吐释放。这需要将这些物质包装到神经元和神经内分泌细胞的特化分泌囊泡中，这一过程由特定的囊泡转运体介导。编码单胺类（VMAT 1和VMAT 2）和乙酰胆碱（VACht）囊泡转运体的基因家族已被克隆并进行了功能鉴定。这些整体膜蛋白的序列预测有十二个跨膜结构域，与一类细菌抗生素抗性蛋白具有较弱的同源性。神经递质分子的囊泡转运已被证明是一种依赖于ATP和质子的主动转运机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

VMAT 1 抗体 (G-12) 参考文献:

  1. 绒毛膜颗粒中的囊泡单胺转运体的生物化学和分子生物学。  |  Henry, JP., et al. 1994. J Exp Biol. 196: 251-62. PMID: 7823026
  2. 推测的乙酰胆碱囊泡转运体的分子克隆。  |  Roghani, A., et al. 1994. Proc Natl Acad Sci U S A. 91: 10620-4. PMID: 7938002
  3. 大鼠大脑突触小泡的乙酰胆碱主动转运。  |  Haigh, JR., et al. 1994. Neuroreport. 5: 773-6. PMID: 8018848
  4. 囊泡单胺转运体的药理特征与多药转运体相似。  |  Yelin, R. and Schuldiner, S. 1995. FEBS Lett. 377: 201-7. PMID: 8543051
  5. 人类囊泡乙酰胆碱转运体对乙酰胆碱的主动转运。  |  Varoqui, H. and Erickson, JD. 1996. J Biol Chem. 271: 27229-32. PMID: 8910293
  6. 囊泡神经递质转运体。调节突触功能的潜在场所。  |  Varoqui, H. and Erickson, JD. 1997. Mol Neurobiol. 15: 165-91. PMID: 9396009
  7. 囊泡神经递质转运和突触前对量子大小的调节  |  Reimer, RJ., et al. 1998. Curr Opin Neurobiol. 8: 405-12. PMID: 9687352

订购信息

产品名称产品编号规格价格数量收藏夹

VMAT 1 抗体 (G-12)

sc-166391
200 µg/ml
RMB2377.00

VMAT 1 (G-12): m-IgGκ BP-HRP 套装

sc-521586
200 µg Ab, 40 µg BP
RMB2662.00

VMAT 1 (G-12): m-IgG2b BP-HRP 套装

sc-548933
200 µg Ab; 10 µg BP
RMB2662.00

VMAT 1 抗体 (G-12) AC

sc-166391 AC
500 µg/ml, 25% agarose
RMB3129.00

VMAT 1 抗体 (G-12) HRP

sc-166391 HRP
200 µg/ml
RMB2377.00

VMAT 1 抗体 (G-12) FITC

sc-166391 FITC
200 µg/ml
RMB2482.00

VMAT 1 抗体 (G-12) PE

sc-166391 PE
200 µg/ml
RMB2580.00

VMAT 1 抗体 (G-12) Alexa Fluor® 488

sc-166391 AF488
200 µg/ml
RMB2685.00

VMAT 1 抗体 (G-12) Alexa Fluor® 546

sc-166391 AF546
200 µg/ml
RMB2685.00

VMAT 1 抗体 (G-12) Alexa Fluor® 594

sc-166391 AF594
200 µg/ml
RMB2685.00

VMAT 1 抗体 (G-12) Alexa Fluor® 647

sc-166391 AF647
200 µg/ml
RMB2685.00

VMAT 1 抗体 (G-12) Alexa Fluor® 680

sc-166391 AF680
200 µg/ml
RMB2685.00

VMAT 1 抗体 (G-12) Alexa Fluor® 790

sc-166391 AF790
200 µg/ml
RMB2685.00

can this antibody cross-react with VMAT-2?

Asked by: BecksBoston
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-06-09

Is this antibody suitable for cell surface staining using flow cytometry? Was this tested?

Asked by: anderkat
Thank you for your question. Anti-VMAT 1 Antibody (G-12) is recommended for detection of VMAT 1 of mouse, rat and human origin by WB, IP, IF and ELISA. This antibody was raised against amino acids 44-143 of human protein, which corresponds to a Lumenal/vesicle topological domain. Fluorescent conjugates can be used for flow cytometry use, but permeabilization of the cells would be required.
Answered by: Tech Support Europe
Date published: 2021-11-02

What is the recommended fixation method for immunofluorescence staining with VMAT 1 (G-12): sc-166391 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Good Western Blot data of VMAT 1 expressionGood Western Blot data of VMAT 1 expression in MIA PaCa-2 whole cell lysate. -SCBT QC
Date published: 2014-12-01
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VMAT 1 抗体 (G-12) is rated 5.0 out of 5 by 2.
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