Date published: 2025-9-8

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Vitronectin 65/75 抗体 (D-8): sc-74484

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说明书
  • Vitronectin 65/75 抗体 D-8 是小鼠单克隆 IgG1 κ,Vitronectin 65/75抗体, 在13篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-270,在Vitronectin 75的human起源的N-terminus处进行映射
  • Vitronectin 65/75 抗体 (D-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Vitronectin 65 and Vitronectin 75
  • 抗Vitronectin 65/75抗体(D-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Vitronectin 65/75 (D-8): sc-74484免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Vitronectin 65/75 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Vitronectin 65/75 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Vitronectin 65/75 抗体(D-8)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的 Vitronectin 65/75 蛋白。Vitronectin 65/75 (D-8) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Vitronectin在细胞外基质中起着至关重要的作用,参与细胞粘附、迁移和伤口愈合过程。Vitronectin的特征在于其RGD(精氨酸-甘氨酸-天冬氨酸)序列,该序列对于与细胞表面的整合素结合至关重要,有助于细胞附着和扩散。这种相互作用在组织修复中尤为重要,因为在伤口愈合过程中,迁移细胞中的Vitronectin水平会升高。Vitronectin能够与纤溶酶原激活剂和整合素结合,这凸显了Vitronectin在调节细胞运动和细胞外基质动态方面的意义。Vitronectin的翻译后修饰(如糖基化)会影响其与其他蛋白质的相互作用,从而影响损伤和修复过程中的细胞反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vitronectin 65/75 抗体 (D-8) 参考文献:

  1. 纤维粘连蛋白受体缺陷细胞在纤维粘连蛋白和玻璃纤维粘连蛋白上的运动性:整合素之间的协同作用。  |  Bauer, JS., et al. 1992. J Cell Biol. 116: 477-87. PMID: 1370495
  2. 纤连蛋白的域结构。活性位点的排列。  |  Høgåsen, K., et al. 1992. J Biol Chem. 267: 23076-82. PMID: 1385412
  3. Vitronectin和胶原蛋白I对间充质干细胞的成骨过程有不同的调控作用。  |  Kundu, AK. and Putnam, AJ. 2006. Biochem Biophys Res Commun. 347: 347-57. PMID: 16815299
  4. 循环免疫复合物(CIC)上存在血浆补体调节蛋白群聚蛋白(Apo J)和玻璃连蛋白(S40)。  |  Chauhan, AK. and Moore, TL. 2006. Clin Exp Immunol. 145: 398-406. PMID: 16907906
  5. 凝血酶裂解玻璃连蛋白。确定纤溶酶原激活剂抑制剂-1的结合位点和随之而来的衰减。  |  Chain, D., et al. 1991. FEBS Lett. 285: 251-6. PMID: 1713175
  6. 关于维蛋白和S蛋白的同一性:免疫交叉反应和功能研究。  |  Mehringer, JH., et al. 1991. Biochem Biophys Res Commun. 179: 655-60. PMID: 1715701
  7. 环磷酸腺苷依赖性蛋白激酶磷酸化纤连蛋白中的丝氨酸378。  |  Clauce-Coupel, H., et al. 2008. Protoplasma. 234: 65-75. PMID: 18841324
  8. 纤维粘连蛋白的结构及其在细胞粘附中的作用。  |  Akiyama, SK., et al. 1981. J Supramol Struct Cell Biochem. 16: 345-8. PMID: 6273596
  9. 纤维粘连蛋白的分子和生物相互作用。  |  Ruoslahti, E., et al. 1982. J Invest Dermatol. 79 Suppl 1: 65s-68s. PMID: 7045246
  10. 对玻璃连蛋白中的精氨酸-甘氨酸-天冬氨酸进行定点突变可消除细胞粘附力。  |  Cherny, RC., et al. 1993. J Biol Chem. 268: 9725-9. PMID: 7683657
  11. 血清素 PAI-1 通过阻断整合素 alpha V beta 3 与玻璃连蛋白的结合来抑制细胞迁移。  |  Stefansson, S. and Lawrence, DA. 1996. Nature. 383: 441-3. PMID: 8837777
  12. 粘附蛋白和反粘附蛋白之间的相互作用对细胞粘附性的不同调节:玻璃粘连蛋白与骨粘连蛋白(BM40, SPARC)结合的特征。  |  Rosenblatt, S., et al. 1997. Biochem J. 324 (Pt 1): 311-9. PMID: 9164872

订购信息

产品名称产品编号规格价格数量收藏夹

Vitronectin 65/75 抗体 (D-8)

sc-74484
200 µg/ml
RMB2377.00

Vitronectin 65/75 (D-8): m-IgG Fc BP-HRP 套装

sc-528673
200 µg Ab; 10 µg BP
RMB2662.00

Vitronectin 65/75 (D-8): m-IgGκ BP-HRP 套装

sc-521122
200 µg Ab, 40 µg BP
RMB2662.00

Vitronectin 65/75 (D-8): m-IgG1 BP-HRP 套装

sc-543062
200 µg Ab; 20 µg BP
RMB2662.00

Vitronectin 65/75 抗体 (D-8) AC

sc-74484 AC
500 µg/ml, 25% agarose
RMB3129.00

Vitronectin 65/75 抗体 (D-8) HRP

sc-74484 HRP
200 µg/ml
RMB2377.00

Vitronectin 65/75 抗体 (D-8) FITC

sc-74484 FITC
200 µg/ml
RMB2482.00

Vitronectin 65/75 抗体 (D-8) PE

sc-74484 PE
200 µg/ml
RMB2580.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 488

sc-74484 AF488
200 µg/ml
RMB2685.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 546

sc-74484 AF546
200 µg/ml
RMB2685.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 594

sc-74484 AF594
200 µg/ml
RMB2685.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 647

sc-74484 AF647
200 µg/ml
RMB2685.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 680

sc-74484 AF680
200 µg/ml
RMB2685.00

Vitronectin 65/75 抗体 (D-8) Alexa Fluor® 790

sc-74484 AF790
200 µg/ml
RMB2685.00

Can you send me a sample of this antibody to try on synaptosomal fraction of human brain? Thank you very much. Vitronectin 65/75 Antibody (D-8): sc-74484

Asked by: svmatv1
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using Vitronectin 65/75 (D-8): sc-74484 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Ms Anti-Vitronectin AbThe Ab worked well on 4% PFA fixed sections of mouse retina
Date published: 2022-04-06
Rated 5 out of 5 by from IF and western both were good.I got a free sample and used it to test for IF and western. It worked good for both. I would now buy a vial for my experiments.
Date published: 2017-12-03
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Positive band observed in WB in A549 andPositive band observed in WB in A549 and HeLa whole cell lysate. -SCBT QC
Date published: 2015-02-04
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Vitronectin 65/75抗体 (D-8) is rated 5.0 out of 5 by 4.
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