Date published: 2025-9-8
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vinculin Antibody (H-10) - Western Blotting - Image 395870
vinculin 抗体 (H-10) Alexa Fluor® 647: sc-25336 AF647. 直接荧光 western 印迹分析 Sol8 (A), A-10 (B), K-562 (C), PC-3 (D), HEL 92. 1. 7 (E) 和 BC3H1 (F) 全细胞裂解液中 vinculin 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
vinculin 抗体 (H-10) Alexa Fluor® 647: sc-25336 AF647. 直接荧光 western 印迹分析 Sol8 (A), A-10 (B), K-562 (C), PC-3 (D), HEL 92. 1. 7 (E) 和 BC3H1 (F) 全细胞裂解液中 vinculin 的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

vinculin 抗体 (H-10): sc-25336

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寫評論提問

说明书
  • vinculin抗体(H-10)是小鼠单克隆IgG2a κ, 在197篇文献中引用，规格为200 µg/ml
  • 免疫human物种的vinculin的氨基酸1-300
  • 抗-vinculin 抗体 (H-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 vinculin
  • 抗vinculin抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取vinculin (H-10): sc-25336的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 vinculin 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与vinculin 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Vinculin抗体（H-10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的Vinculin。抗微丝蛋白抗体（H-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Vinculin在细胞粘附和机械转导中起着至关重要的作用，主要位于连接细胞外基质和肌动蛋白细胞骨架的特殊结构--粘着斑中。这些粘着斑对于维持细胞形状、运动和信号传递至关重要，因为它们有助于在细胞及其环境之间传递机械力和生化信号。Vinculin与其他细胞骨架蛋白（如Talin和α-actinin）的相互作用对于这些粘附复合物的组装和稳定性至关重要。此外，vinculin与肌动蛋白和其他粘附蛋白结合的能力凸显了其在调节细胞对机械应力的反应和促进细胞迁移方面的重要性，使vinculin成为伤口愈合和组织修复等过程中的关键参与者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

vinculin 抗体 (H-10)

sc-25336
200 µg/ml
RMB2377.00

vinculin (H-10): m-IgG Fc BP-HRP 套装

sc-525517
200 µg Ab; 10 µg BP
RMB2662.00

vinculin (H-10): m-IgG2a BP-HRP 套装

sc-546300
200 µg Ab; 10 µg BP
RMB2662.00

vinculin 抗体 (H-10) AC

sc-25336 AC
500 µg/ml, 25% agarose
RMB3129.00

vinculin 抗体 (H-10) HRP

sc-25336 HRP
200 µg/ml
RMB2377.00

vinculin 抗体 (H-10) FITC

sc-25336 FITC
200 µg/ml
RMB2482.00

vinculin 抗体 (H-10) PE

sc-25336 PE
200 µg/ml
RMB2580.00

vinculin 抗体 (H-10) Alexa Fluor® 488

sc-25336 AF488
200 µg/ml
RMB2685.00

vinculin 抗体 (H-10) Alexa Fluor® 546

sc-25336 AF546
200 µg/ml
RMB2685.00

vinculin 抗体 (H-10) Alexa Fluor® 594

sc-25336 AF594
200 µg/ml
RMB2685.00

vinculin 抗体 (H-10) Alexa Fluor® 647

sc-25336 AF647
200 µg/ml
RMB2685.00

vinculin 抗体 (H-10) Alexa Fluor® 680

sc-25336 AF680
200 µg/ml
RMB2685.00

vinculin 抗体 (H-10) Alexa Fluor® 790

sc-25336 AF790
200 µg/ml
RMB2685.00

Dear Sir/Madam, I'm Ahmed Soliman, a PhD student at the University of Groningen and I'm interested in your product,vinculin Antibody (H-10) HRP. Would it be possible to get a free 10 µg sample of it ?

Asked by: Soliman
Thank you for your question. Yes, we would be happy to sen you a sample of this antibody, however we do not offer samples of the conjugated form, so only the standard (non-HRP) form is offered as a sample. Please contact our Technical support group by phone, email ([email protected]) or via live chat on our website, www.scbt.com, and we would be happy to assist you.
Answered by: Tech Service
Date published: 2017-12-07

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2017-01-06
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Rated 3 out of 5 by from satisfiedwe used this antibody in western blot, the incubation was 1 hour long at room temperature and the antibody was diluted 1:200. We run extracts from PEL lines (BC3 and BCBL1) on the gel
Date published: 2021-02-11
Rated 4 out of 5 by from WB in H9c2 cellsAnticorpo perfettamente funzionante su estratti totali di H9C2. Blocking con no-fat milk al 5% per un'ora. Anti-vinculin (H-10) 1:500 Overnight. Goat anti-Mouse 1:2000 per un'ora a temperatura ambiente
Date published: 2020-11-09
Rated 5 out of 5 by from SC-25336High sensitivity and specificity with stable quality
Date published: 2019-10-02
Rated 2 out of 5 by from Band is very faint.The antibody showed up in Huh7 cells but the band was very faint compared to vinculin (7F9), which was very robust in multiple experiments.
Date published: 2017-12-15
Rated 4 out of 5 by from good vinculin antibodyI use this antibody in a dilution 1:200 in T98G mammalian cells overnight in milk PBS-Tween. However, it works better the vinculin sc-73614
Date published: 2017-11-15
Rated 5 out of 5 by from Great antibodyExcellent antibody for Western blot using hASMC extract.
Date published: 2017-11-14
Rated 5 out of 5 by from Vinculin Antibody (sc-25336)I used this vinculin Antibody for the western. It gave me nice clean signal. I am very much satisfied with this antibody.
Date published: 2017-09-27
Rated 3 out of 5 by from Not recommended for stem cellsAlthough it worked fine for differentiated cells, I strongly suggest not to use it for stem cells. Also, a lower dilution was needed in order to see the bands clearly.
Date published: 2017-08-31
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vinculin 抗体 (H-10) is rated 4.4 out of 5 by 16.
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