Date published: 2025-9-6
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Vimentin Antibody (V9) - Western Blotting - Image 390382
波形蛋白抗体 (V9): sc-6260. U-251-MG (A), Jurkat (B), HeLa (C), A-10 (D) 和 KNRK (E) 全细胞裂解液中波形蛋白表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 555: sc-516177.
波形蛋白抗体 (V9): sc-6260. U-251-MG (A), Jurkat (B), HeLa (C), A-10 (D) 和 KNRK (E) 全细胞裂解液中波形蛋白表达的荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 555: sc-516177.

Vimentin 抗体 (V9): sc-6260

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说明书
  • Vimentin 抗体 V9 是小鼠单克隆 IgG1 κ，Vimentin抗体, 在1,158篇文献中引用，规格为200 µg/ml
  • 来自porcine眼晶状体的纯化Vimentin
  • Vimentin 抗体 (V9) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Vimentin; 也和以下物种反应，包括： porcine
  • 抗Vimentin抗体（V9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Vimentin (V9): sc-6260的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Vimentin 抗体 (V9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Vimentin 抗体 (V9) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Vimentin抗体（V9）是一种小鼠单克隆IgG1 kappa轻链抗体，针对猪眼晶状体中的纯化vimentin（一种主要在间充质细胞中表达的关键细胞骨架蛋白）产生。Vimentin小鼠单克隆抗体（V9）识别vimentin免疫原序列内的表位，并在蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）应用中表现出广泛的物种交叉反应性，包括小鼠、大鼠和人类样本。抗波形蛋白单克隆抗体（V9）有非结合型和多种结合型，包括辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素、琼脂糖和多种Alexa Fluor®结合物，可满足各种实验需求。波形蛋白属于中间丝家族，可维持细胞结构、完整性和机械弹性。Vimentin与包括细胞角蛋白、肌动蛋白和微管在内的多种蛋白质相互作用，形成复杂的细胞骨架纤维网络。Vimentin与运动蛋白的结合有助于细胞内运输和细胞器定位。Vimentin主要存在于细胞质中，但在特定条件下也会定位到细胞核和细胞膜。Vimentin是上皮细胞向间充质细胞转化（EMT）的关键标志物，这一过程对于伤口愈合、发育和肿瘤转移至关重要。在癌症生物学中，Vimentin的表达模式变得尤为重要，因为Vimentin在包括黑色素瘤、肉瘤和乳腺癌在内的各种肿瘤中经常出现上调，在这些肿瘤中，Vimentin通常与细胞角蛋白和其他中间丝共同表达。Vimentin (V9)抗体使研究人员能够通过Vimentin一致的细胞质和膜染色特性来研究细胞骨架动力学、癌症进展和组织特异性表达模式。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vimentin 抗体 (V9) 参考文献:

  1. 通过单克隆抗体确定的波形蛋白和 desmin 的共同抗原决定因子。  |  Dráberová, E., et al. 1986. Folia Biol (Praha). 32: 295-303. PMID: 2465190
  2. 人发育期脊髓中波形蛋白和胶质纤维酸性蛋白的表达。  |  Lukás, Z., et al. 1989. Histochem J. 21: 693-701. PMID: 2482269
  3. Vimentin调节NLRP3炎症小体的激活。  |  dos Santos, G., et al. 2015. Nat Commun. 6: 6574. PMID: 25762200
  4. 健康和疾病中的纤丝蛋白多样性。  |  Danielsson, F., et al. 2018. Cells. 7: PMID: 30248895
  5. 中间丝多肽在人类胎儿和成人组织中的共表达。  |  Van Muijen, GN., et al. 1987. Lab Invest. 57: 359-69. PMID: 3669613
  6. 细胞外纤连蛋白作为癌症中的一种多功能免疫抑制蛋白。  |  van Loon, K., et al. 2023. Biochim Biophys Acta Rev Cancer. 1878: 188985. PMID: 37717859
  7. 硬α-角蛋白 IF：一种缺乏头尾分子重叠的结构模型，但具有表皮角蛋白和波形蛋白 IF 的混合特征。  |  Parry, DA. 1995. Proteins. 22: 267-72. PMID: 7479699
  8. 从小鼠胚胎中间丝基因调控中可以学到什么？  |  Duprey, P. and Paulin, D. 1995. Int J Dev Biol. 39: 443-57. PMID: 7577435
  9. 人类发育中背根神经节和脊髓中磷酸化高分子量神经丝蛋白（NF-H）和波形蛋白的表达。  |  Lukás, Z., et al. 1993. Histochemistry. 100: 495-502. PMID: 8163392
  10. 中间纤维结构和组装。  |  Stewart, M. 1993. Curr Opin Cell Biol. 5: 3-11. PMID: 8448027
  11. 使用 Vim 3B4 和 V9 单克隆抗体进行免疫细胞化学研究，揭示神经胶质波形蛋白的物种特异性。  |  Gereben, B., et al. 1995. Neurobiology (Bp). 3: 151-64. PMID: 8563714
  12. 简单上皮细胞类型中波形蛋白表达增加的结构和生物学后果。  |  Andreoli, JM. and Trevor, KT. 1995. Cell Motil Cytoskeleton. 32: 10-25. PMID: 8674130

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (V9)

sc-6260
200 µg/ml
RMB2377.00

Vimentin (V9): m-IgGκ BP-HRP 套装

sc-520474
200 µg Ab, 40 µg BP
RMB2662.00

Vimentin 抗体 (V9) AC

sc-6260 AC
500 µg/ml, 25% agarose
RMB3129.00

Vimentin 抗体 (V9) HRP

sc-6260 HRP
200 µg/ml
RMB2377.00

Vimentin 抗体 (V9) FITC

sc-6260 FITC
200 µg/ml
RMB2482.00

Vimentin 抗体 (V9) PE

sc-6260 PE
200 µg/ml
RMB2580.00

Vimentin 抗体 (V9) Alexa Fluor® 488

sc-6260 AF488
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) Alexa Fluor® 546

sc-6260 AF546
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) Alexa Fluor® 594

sc-6260 AF594
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) Alexa Fluor® 647

sc-6260 AF647
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) Alexa Fluor® 680

sc-6260 AF680
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) Alexa Fluor® 790

sc-6260 AF790
200 µg/ml
RMB2685.00

Vimentin 抗体 (V9) TRITC

sc-6260 TRITC
200 µg/ml
RMB2407.00

Vimentin 抗体 (V9) Alexa Fluor® 405

sc-6260 AF405
200 µg/ml
RMB2685.00

What is the KD (dissociation constant) of this antibody?

Asked by: Grace123456789
Thank you for your question. We do not measure or determine dissociation constant for our antibodies .We do know some are higher affinity than others ,but we so not measure or analyze this information.
Answered by: BlakeJ
Date published: 2024-05-12

可以偶联BV510或者bv711或者bv405吗

Asked by: yiwang
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I stained a cancer cell line with this mouse antibody and a rabbit one, but this didn't only work even at 1:50. Do you recommend a directly conjugated mouse monoclonal primary antibody, and why?

Asked by: divingbeetle
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the epitope used for Vimentin V9 antibody? Is it the same as the old vimentin C20 or H84?

Asked by: Yuni
Thank you for your question. Vimentin Antibody (V9): sc-6260 has been raised against purified Vimentin from porcine eye lens, but its exact epitope region has not been determined, so its epitope is not known. We also offer Vimentin Antibody (E-5): sc-373717, which is specific amino acids 419-438 near the C-terminus of Vimentin of human origin.
Answered by: Tech Support Europe
Date published: 2020-10-30

请问这样获得vimentin小样？

Asked by: 爱你金灿灿
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website. 感谢您的提问。如何获取免费试用装，请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-09

Is it possible to order a sample of unconjugated Vimentin Antibody (V9) before purchasing?

Asked by: gradstudent21
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am researcher at Trinity College Dublin, Ireland. My work is mainly focused on CTCs, and planning to detect Vimentin marker. Could I get your free sample of FITC- labelled anti-vimentin Ab (Santa-Cruz, ’vimentin cohort’)? Thanks very much. Yanmei

Asked by: Yanmei
Thank you for your question. Unfortunately, we don't offer samples of conjugated antibodies. Please contact our Technical Service Department to request a free 10 µg sample of our mouse monoclonal antibody, AOS-1 (H-9): sc-398080. Please call our European Support office toll-free at 00800 4573 8000 or email to [email protected]
Answered by: Technical Support Europe
Date published: 2017-07-21

Can you confirm if the concentration stays the same from lot to lot?

Asked by: CRUK SH
Thank you for your question. The concentration of IgG provided in each lot is the same. We provide this Vimentin (V9) mouse monoclonal IgG1 at a concentration of 200 µg in 1 ml.
Answered by: Technical Support
Date published: 2017-03-09
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Rated 5 out of 5 by from Excellent antibodyI used anti-vimentin (1:200) and worked perfectly in my breast cancer cell lysates (WB)
Date published: 2023-11-21
Rated 5 out of 5 by from Great for WBProvides a very strong signal in WB (tested in human cells, 1:2000 dilution).
Date published: 2023-10-15
Rated 5 out of 5 by from Great signal, highly specific and with fast timesWe tested the sample for the mouse monoclonal antibody for Vimentin from Santa Cruz Biotechnology. We are very pleased with the results, as the antibody performed exceptionally well in our experimental conditions. Nitrocellulose membrane with our total protein extracts transferred onto it was incubated for 1 hour with 5% milk solution in TBS-T, to block non-specific binding sites. Then, primary antibody sc-6260 was incubated for 2 hours at RT diluted 1:1000 in TBS-T with 0.02% Sodium Azide. Lastly, secondary anti-mouse antibody was incubated for 1 hour at RT diluted 1:2000 in 3% milk solution in TBS-T. HRP signal was detected with ECL incubation and quick film development. The signal appeared as a single specific band detected at around 57kDa, as indicated in the datasheet
Date published: 2023-06-19
Rated 1 out of 5 by from Does not work!Antibody did not work in PFA fixed mouse brain tissue immunofluorescent staining. Used multiple dilutions and tried citrate boiling pH 6 unmasking.
Date published: 2022-10-24
Rated 5 out of 5 by from Great features for IHQThis antibody showed a good marked in neoplasm slices
Date published: 2022-08-31
Rated 5 out of 5 by from It works!This antibody gave a strong and clean signal when we used it at a 1:200 diluition with 40μg of protein samples.
Date published: 2022-05-26
Rated 5 out of 5 by from Good antibody for Western blotIt works great - I'd recommend this Vimentin antibody
Date published: 2022-05-05
Rated 1 out of 5 by from Not a good antibodyI bought this antibody recently and the antibody is so weak, it does not recognize vimentin even when used at 1:500 dilution.
Date published: 2022-02-08
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Vimentin 抗体 (V9) is rated 4.6 out of 5 by 79.
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