Date published: 2025-9-6

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Vimentin 抗体 (J144): sc-53464

4.8(5)
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说明书
  • Vimentin 抗体 J144 是小鼠单克隆 IgM, 在31篇文献中引用,提供 200 µg/ml
  • 针对human来源的横纹肌肉瘤细胞系JR1
  • 推荐用于 mouse, rat 和 human 来源的Vimentin WB, IP, IF 和 IHC(P)检测
  • 看 Vimentin (E-5): sc-373717,可查看有标记的 Vimentin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Vimentin 抗体 (J144)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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Vimentin 抗体(J144)是一种小鼠IgM单克隆抗体,免疫原信息为人源性横纹肌肉瘤细胞系JR1。该抗体对Vimentin具有高度特异性,Vimentin是一种主要在间充质来源细胞中表达的关键中间丝蛋白。Vimentin对于维持细胞完整性、提供机械支撑以及促进细胞迁移等过程至关重要,特别是在上皮细胞向间充质细胞转化(EMT)和肿瘤转移期间。重要的是,Vimentin经历多种翻译后修饰,包括磷酸化、乙酰化和泛素化,以调节其功能。在细胞分裂过程中,Vimentin在Ser38和Ser55等位点的磷酸化至关重要,它可调节丝状物的分解和重组,从而实现正常的细胞分裂。Vimentin的乙酰化会影响其稳定性和丝状物的组装,从而影响细胞对压力的反应。另一方面,泛素化作用可导致Vimentin降解,从而影响细胞在动态环境中的形态和流动性。Vimentin还与肌动蛋白等关键蛋白相互作用,为肌肉细胞提供结构完整性,并与β-catenin相互作用,影响细胞粘附和与肿瘤发生相关的信号通路。克隆号为J144的抗体可用于石蜡包埋切片免疫组织化学(IHC)、免疫荧光(IF)、免疫沉淀(IP)和蛋白免疫印迹(WB)应用,为癌症研究、EMT和细胞骨架动力学研究提供了广泛的用途。该抗体有非偶联形式和偶联形式,偶联形式包括HRP和FITC,可在各种实验中广泛使用。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (J144)

sc-53464
200 µg/ml
RMB2377.00

Are there any issues with using sc-53464: Vimentin (J144) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53464 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Very good antibodyVimentin Antibody (J144) worked well by Western blot. This antibody produced strong and specific band
Date published: 2017-01-31
Rated 4 out of 5 by from HCT116 CRC and SW480 CRC cellsHCT116 CRC and SW480 CRC cells - more antibody gave better signals
Date published: 2016-01-21
Rated 5 out of 5 by from Publishable data for use in IF in human cervicalPublishable data for use in IF in human cervical fibroblast. -SCBT Publication Review
Date published: 2015-01-05
Rated 5 out of 5 by from Publishable data for use in WBPublishable data for use in WB, IF, and IHC in human lung adenocarcinoma. -SCBT Publication Review
Date published: 2015-01-02
Rated 5 out of 5 by from Strong specific band in WB in SJRH30 andStrong specific band in WB in SJRH30 and RD whole cell lysates. -SCBT QC
Date published: 2014-09-27
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Vimentin 抗体 (J144) is rated 4.8 out of 5 by 5.
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