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Vimentin Antibody (E-5) - Western Blotting - Image 390727
波形蛋白抗体 (E-5) Alexa Fluor® 594: sc-373717 AF594. MOLT-4 (A), Jurkat (B), HeLa (C), C3H/10T1/2 (D) 和 NIH/3T3 (E) 全细胞裂解液中波形蛋白表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
波形蛋白抗体 (E-5) Alexa Fluor® 594: sc-373717 AF594. MOLT-4 (A), Jurkat (B), HeLa (C), C3H/10T1/2 (D) 和 NIH/3T3 (E) 全细胞裂解液中波形蛋白表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Vimentin 抗体 (E-5): sc-373717

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寫評論提問

说明书
  • Vimentin抗体(E-5)是小鼠单克隆IgG1 κ, 在212篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Vimentin的C-terminus附近的氨基酸419-438之间
  • 抗-Vimentin 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Vimentin; 也和以下物种反应，包括： 和 canine and bovine
  • 抗Vimentin抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Vimentin (E-5): sc-373717的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Vimentin Antibody (E-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与Vimentin Antibody (E-5) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

波形蛋白抗体（E-5）是一种IgG1κ小鼠单克隆波形蛋白抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的波形蛋白。波形蛋白抗体（E-5）可作为非偶联的抗波形蛋白抗体形式，以及多种偶联形式的抗波形蛋白抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞骨架中间丝（IFs）由高度组织特异性表达的多种蛋白质组成。中间丝由排列在不完全螺旋晶格上的双链α螺旋卷曲分子构成，已被广泛用作区分组织内单个细胞类型和鉴定转移性肿瘤起源的标记。一种这样的中间丝蛋白，波形蛋白，是起源于间充质细胞的细胞的通用标记。波形蛋白经常与中间丝家族的其他成员，如细胞角蛋白，在包括黑色素瘤和乳腺癌在内的肿瘤中共同表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vimentin 抗体 (E-5) 参考文献:

  1. 波形蛋白细胞骨架信号转导中 p21 激活激酶在 Ser-56 处磷酸化波形蛋白的关键作用。  |  Li, QF., et al. 2006. J Biol Chem. 281: 34716-24. PMID: 16990256
  2. Vimentin支持线粒体的形态和组织。  |  Tang, HL., et al. 2008. Biochem J. 410: 141-6. PMID: 17983357
  3. 纤丝蛋白破坏对关节软骨细胞力学反应的影响。  |  Chen, C., et al. 2016. Biochem Biophys Res Commun. 469: 132-137. PMID: 26616052
  4. 乳腺癌中 Vimentin 和 Axl 表达的影响。  |  Tanaka, K., et al. 2016. Clin Breast Cancer. 16: 520-526.e2. PMID: 27506606
  5. 波形蛋白调节人类乳头瘤病毒 16 伪病毒的感染性内化  |  Schäfer, G., et al. 2017. J Virol. 91: PMID: 28566373
  6. 上皮波形蛋白在乳腺发育过程中发挥着功能性作用。  |  Peuhu, E., et al. 2017. Development. 144: 4103-4113. PMID: 28947532
  7. 蛋白质组分析显示波形蛋白参与胶质母细胞瘤细胞迁移  |  Nowicki, MO., et al. 2019. Cancers (Basel). 11: PMID: 30987208
  8. 外源性Vimentin补充暂时影响HPV16假病毒感染的早期步骤。  |  Carse, S., et al. 2021. Viruses. 13: PMID: 34960740
  9. vimentin免疫表达及其在结肠癌白种人患者中的预后活性。  |  Brzozowa-Zasada, M., et al. 2022. Prz Gastroenterol. 17: 123-129. PMID: 35664018
  10. 细胞外纤维蛋白改变能量代谢并诱导脂肪细胞肥大。  |  Park, JH., et al. 2024. Diabetes Metab J. 48: 215-230. PMID: 37750184

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (E-5)

sc-373717
200 µg/ml
RMB2377.00

Vimentin (E-5): m-IgGκ BP-HRP 套装

sc-522468
200 µg Ab, 40 µg BP
RMB2662.00

Vimentin 抗体 (E-5) AC

sc-373717 AC
500 µg/ml, 25% agarose
RMB3129.00

Vimentin 抗体 (E-5) HRP

sc-373717 HRP
200 µg/ml
RMB2377.00

Vimentin 抗体 (E-5) FITC

sc-373717 FITC
200 µg/ml
RMB2482.00

Vimentin 抗体 (E-5) PE

sc-373717 PE
200 µg/ml
RMB2580.00

Vimentin 抗体 (E-5) Alexa Fluor® 488

sc-373717 AF488
200 µg/ml
RMB2685.00

Vimentin 抗体 (E-5) Alexa Fluor® 546

sc-373717 AF546
200 µg/ml
RMB2685.00

Vimentin 抗体 (E-5) Alexa Fluor® 594

sc-373717 AF594
200 µg/ml
RMB2685.00

Vimentin 抗体 (E-5) Alexa Fluor® 647

sc-373717 AF647
200 µg/ml
RMB2685.00

Vimentin 抗体 (E-5) Alexa Fluor® 680

sc-373717 AF680
200 µg/ml
RMB2685.00

Vimentin 抗体 (E-5) Alexa Fluor® 790

sc-373717 AF790
200 µg/ml
RMB2685.00

Vimentin (E-5) 中和勝肽

sc-373717 P
100 µg/0.5 ml
RMB511.00

Dose Sc-373717 contain 0.5% BSA?

Asked by: Song
Thank you for your question. Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: BlakeJ
Date published: 2024-06-12

would it be possible to tell me the epitope?

Asked by: Thanos
Thank you for your question. Anti-Vimentin Antibody (E-5): sc-373717 is specific for an epitope mapping between amino acids 419-438 near the C-terminus of Vimentin of human origin.
Answered by: Tech Support Europe
Date published: 2021-10-26

Is there any reactivity with lower vertebrates, such as frog or salamanders?

Asked by: Newt83
Thank you for your question! Anti-Vimentin Antibody (E-5) is recommended for detection of Vimentin of mouse, rat, human, canine and bovine origin by WB, IP, IF, IHC(P) and ELISA. There is no data supporting reactivity with samples from non-mammalian or amphibian species.
Answered by: Tech Support Europe
Date published: 2021-07-29

Is there data showing reactivity with canine samples?

Asked by: AbPolly
Thank you for your inquiry. We do not have data showing reactivity of this antibody with canine samples, the recommendation for reactivity with canine samples is made based on 100% homology of the epitope of the antibody with the sequence for canine Vimentin.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 5 out of 5 by from Works fineIt works fine for immunofluorescence on paraffin-embedded human heart valve samples.
Date published: 2021-02-03
Rated 5 out of 5 by from Works perfectly in immunofluorescenceClear and very specific staining in murine retina.
Date published: 2020-01-28
Rated 5 out of 5 by from IF: Good staining!Good staining (IF 1:50/1:100/1:200) on cryosections (10µm) from human retinal organoids (for all dilutions)!
Date published: 2019-07-23
Rated 5 out of 5 by from 非常好我们申请了试用装，结果很好。肝癌细胞全蛋白裂解液，1：800稀释，一条带，无背景，推荐购买。我发现个问题，如果用中文回复，要写好多字。
Date published: 2019-03-05
Rated 5 out of 5 by from Works well at 1:50 for immunofluorescenceGood staining using 1:50 for immunofluorescence in primary fibroblasts
Date published: 2018-08-10
Rated 4 out of 5 by from Good productIt is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
Rated 3 out of 5 by from Vimentin Antibody (E-5)Vimentin Antibody (E-5): sc. Near-infrared western blot analysis of LDH-A expression in MEF whole cell lysates. Blocked with 5% NFDM for 1 hour. Detection reagent used: DAM CW800 (Licor).
Date published: 2018-06-28
Rated 4 out of 5 by from きれいにバンドを出すことができました。Ishikawa細胞 10 μg を泳動しました。非特異的なバンドもでてしまいますが、比較的きれいにバンドを出すことができました。
Date published: 2018-03-16
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Vimentin 抗体 (E-5) is rated 4.8 out of 5 by 37.
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