Date published: 2026-10-28

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Vimentin 抗体 (9E7E7): sc-66001

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说明书
  • Vimentin抗体(9E7E7)是小鼠单克隆IgG1 κ, 在24篇文献中引用,规格为200 µg/ml
  • 针对纯化的截短重组human的Vimentin
  • 推荐用于 human 来源的Vimentin WB, IP, IF 和 IHC(P)检测
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Vimentin Antibody (9E7E7) for WB and IHC(P) applications. 的首选辅助检测试剂,这些试剂现与Vimentin Antibody (9E7E7) 打包提供(请参阅下面的订购信息)。
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Vimentin (9E7E7) 抗体是一种小鼠IgG1 kappa抗体,针对纯化的、来自人类的截短重组体Vimentin,适用于免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHC(P))检测人类Vimentin。Vimentin是细胞骨架中间丝(IF)的关键组成部分,它提供结构完整性,并参与细胞迁移、伤口愈合和上皮-间质转化(EMT)等关键细胞功能。这种蛋白质经常被用作间充质细胞的一般标记,并且经常与其他中间丝蛋白(如细胞角蛋白)共同表达,特别是在黑色素瘤和乳腺癌等癌症中。 维明坦与各种蛋白质相互作用,包括整合素、微丝蛋白和AKT,这些蛋白质对于细胞黏合、信号传导和细胞凋亡调节至关重要。与整合素的相互作用在细胞迁移中起着重要作用,而与微丝蛋白的缔合作用则通过将微丝蛋白与其他细胞骨架成分连接起来,从而提供结构稳定性。AKT的磷酸化作用调节了微丝蛋白在细胞存活和运动中的功能,而细胞存活和运动是癌症进展和转移的关键因素。克隆9E7E7抗体是识别间充质细胞和区分肿瘤来源的宝贵工具,使其在癌症和转移研究中的应用非常有效。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Vimentin 抗体 (9E7E7) 参考文献:

  1. 纤连蛋白在细胞粘附、迁移和信号传导中的新功能。  |  Ivaska, J., et al. 2007. Exp Cell Res. 313: 2050-62. PMID: 17512929
  2. Vimentin DNA甲基化预测乳腺癌的存活率。  |  Ulirsch, J., et al. 2013. Breast Cancer Res Treat. 137: 383-96. PMID: 23239149
  3. 波形蛋白通过激活 NLRP3 促进内皮炎症。  |  Zhou, HF., et al. 2020. Int J Cardiol. 301: 155. PMID: 31948594
  4. 脂肪细胞祖细胞外泌体中的波形蛋白可加速伤口愈合。  |  Parvanian, S., et al. 2020. Cytoskeleton (Hoboken). 77: 399-413. PMID: 32978896
  5. 波形蛋白中间丝与 Caveolin-1 的单向调节。  |  Shi, X., et al. 2020. Int J Mol Sci. 21: PMID: 33050149
  6. 链球菌自溶素通过与波形蛋白相互作用促进猪气管上皮功能障碍。  |  Meng, Y., et al. 2022. PLoS Pathog. 18: e1010765. PMID: 35921364
  7. 细胞骨架中的波形蛋白通过mTORC1信号传导来调节细胞大小和自噬。  |  Mohanasundaram, P., et al. 2022. PLoS Biol. 20: e3001737. PMID: 36099296
  8. 膜结合纤连蛋白丝在应激下重组和伸长。  |  Nageswaran, S., et al. 2023. Biomacromolecules. 24: 2512-2521. PMID: 37132386
  9. 曲美替尼(Trametinib)作为细胞骨架中纤丝蛋白重塑的协调者的发现。  |  Zhao, S., et al. 2024. J Mol Cell Biol. 16: PMID: 38429984
  10. Vimentin调节线粒体ROS的产生和中性粒细胞的炎症反应。  |  Huynh, TN., et al. 2024. Front Immunol. 15: 1416275. PMID: 39139560

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (9E7E7)

sc-66001
200 µg/ml
CNY2422.00

Vimentin (9E7E7): m-IgG Fc BP-HRP 套装

sc-539206
200 µg Ab; 10 µg BP
CNY2715.00

Vimentin (9E7E7): m-IgG1 BP-HRP 套装

sc-541324
200 µg Ab; 20 µg BP
CNY2715.00

I ordered sc-66001 Ab from you. Does this Ab contain any glycine, Tris, glycerol, if yes what are the concentrations of them?

Asked by: Hong
Thank you for your question. Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: BlakeJ
Date published: 2024-06-14

I am using Vimentin (9E7E7): sc-66001 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by TO from Great immunoperoxidase cytoplasmic and membraneGreat immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by LF from Robust bands in WB in SJRH30 and RD wholeRobust bands in WB in SJRH30 and RD whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by TS from Publishable data for use in IHC (PFA) inPublishable data for use in IHC (PFA) in human oral cavity tumor sample. -SCBT Publication Review
Date published: 2015-01-27
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Vimentin 抗体 (9E7E7) is rated 5.0 out of 5 by 3.
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