Date published: 2025-9-6

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Vimentin 抗体 (3CB2): sc-80975

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说明书
  • Vimentin抗体(3CB2)是小鼠单克隆IgM κ, 在43篇文献中引用,规格为200 µg/ml
  • 与Vimentin反应,从chicken胚胎脑组织中提取
  • 推荐用于 chicken, human, mouse 和 rat 来源的Vimentin WB, IP, IF 和 IHC(P)检测
  • 看 Vimentin (E-5): sc-373717,可查看有标记的 Vimentin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP 是 Vimentin 抗体 (3CB2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Vimentin 抗体 (3CB2) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

Vimentin (3CB2) 抗体是一种小鼠IgM单克隆抗体,针对从鸡胚胎脑组织中提取的Vimentin。Vimentin是一种57 kDa的蛋白质,在维持细胞完整性和抵抗间充质来源细胞的机械应力方面发挥着关键作用。作为细胞骨架中间丝(IF)家族的一员,Vimentin具有独特的结构,由双链α螺旋卷曲分子组成,这些分子组装成更大的丝状网络,支撑细胞的形状和细胞器定位。这种丝状网络不仅对结构支撑至关重要,而且有助于细胞内转运,并在细胞迁移、分裂和信号传导等过程中稳定细胞成分。尽管在细胞分裂或特定压力条件下,Vimentin也会出现在其他区域,但它主要定域于细胞质中。它的表达受到严格调控,并参与影响细胞运动、黏合和凋亡的信号传导通路。在癌细胞进展、纤维化和伤口愈合的关键过程--上皮-间质转化(EMT)中,纤连蛋白通常会上调。在各种肿瘤中,它经常与细胞角蛋白等其他中间丝共表达,因此成为识别转移性癌症(包括黑色素瘤和乳腺癌)起源的重要标志物。这种抗体广泛用于研究和诊断,以区分间充质来源的细胞,对于研究涉及细胞骨架功能障碍的疾病也很有价值。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Vimentin 抗体 (3CB2)

sc-80975
200 µg/ml
RMB2377.00

Vimentin (3CB2): m-IgGκ BP-HRP 套装

sc-550737
200 µg Ab; 40 µg BP
RMB2662.00

I am using Vimentin (3CB2): sc-80975 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
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Rated 4 out of 5 by from A crisp band was observed by Western BlotA crisp band was observed by Western Blot of the endogenously expressed vinculin protein in HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Produced great immunofluorescence cytoskeletalProduced great immunofluorescence cytoskeletal staining in paraformaldehyde-fixed cultured human U-87 glioma cells. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Multiple bands (protein is glycosylated andMultiple bands (protein is glycosylated and phosphorylated post translation) observed by Western Blot using detergent insoluble extracts from human U-87 glioma cells. -SCBT QC
Date published: 2015-02-03
Rated 4 out of 5 by from Specificity demonstrated by Western BlotSpecificity demonstrated by Western Blot comparing 293T cells that have been transfected to over-express the human vinculin protein to those that have not. A definite increase in expression post transfection is observed. -SCBT QC
Date published: 2015-02-02
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Vimentin 抗体 (3CB2) is rated 4.0 out of 5 by 4.
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