Date published: 2025-9-7
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Villin Antibody (1D2C3) - Western Blotting - Image 393168
Villin 抗体 (1D2C3) Alexa Fluor® 680: sc-58897 AF680. Caco-2 (A), HCT-8 (B), HT-29 (C) 和 Hep G2 (D) 全细胞裂解液中 Villin 表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
Villin 抗体 (1D2C3) Alexa Fluor® 680: sc-58897 AF680. Caco-2 (A), HCT-8 (B), HT-29 (C) 和 Hep G2 (D) 全细胞裂解液中 Villin 表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

Villin 抗体 (1D2C3): sc-58897

4.9(8)
寫評論提問

说明书
  • Villin 抗体 1D2C3 是小鼠单克隆 IgG1 κ，Villin抗体, 在81篇文献中引用，规格为200 µg/ml
  • 与纯化的全长天然 Villin chicken 起源
  • Villin 抗体 (1D2C3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human 和avian 来源的 Villin; 不会与 breast cancer产生交联反应
  • 抗Villin抗体（1D2C3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Villin (1D2C3): sc-58897的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Villin 抗体 (1D2C3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Villin 抗体 (1D2C3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Villin抗体（1D2C3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠、人类和禽类来源的Villin蛋白。抗Villin抗体（1D2C3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。微丝蛋白在组织肌动蛋白细胞骨架方面发挥着至关重要的作用，特别是在吸收上皮细胞的刷状缘，微丝蛋白有助于维持细胞形状和吸收面积。微丝蛋白的活性受钙离子调节，钙离子影响肌动蛋白丝的结合和断裂，促进细胞骨架的动态变化，这对细胞运动和形态至关重要。Villin与其他肌动蛋白结合蛋白（如Caldesmon、Filamin 1和Nebulin）的相互作用凸显了Villin在细胞骨架调节中的重要性，使其成为肌肉组织和非肌肉组织中的重要组成部分。Villin的差异表达和调节强调了其在各种细胞功能中的作用，突出了其在维持不同物种细胞完整性和功能中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Villin 抗体 (1D2C3) 参考文献:

  1. 小鼠消化道和泌尿道上皮细胞系中绒毛蛋白基因表达的发育调控。  |  Maunoury, R., et al. 1992. Development. 115: 717-28. PMID: 1425351
  2. Caldesmon 可与平滑肌肌球蛋白和肌球蛋白杆结合，并使粗丝与肌动蛋白丝交联。  |  Marston, S., et al. 1992. J Muscle Res Cell Motil. 13: 206-18. PMID: 1534566
  3. Villin expression is frequently lost in poorly differentiated colon cancer.  |  Arango, D., et al. 2012. Am J Pathol. 180: 1509-21. PMID: 22349300
  4. 从刷状缘的肌动蛋白结合蛋白微绒毛蛋白的结构到功能。  |  Friederich, E., et al. 1990. Bioessays. 12: 403-8. PMID: 2256904
  5. Site-specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein-mediated membrane fusion.  |  Chandra, S., et al. 2019. FEBS Lett. 593: 1927-1943. PMID: 31183850
  6. Villin is a biomarker for reverse polarity in colorectal micropapillary carcinoma.  |  Zhao, L., et al. 2021. Oncol Lett. 21: 72. PMID: 33365083
  7. HeLa 细胞丝胶的肌动蛋白结合和二聚化结构域。  |  Weihing, RR. 1988. Biochemistry. 27: 1865-9. PMID: 3378035
  8. Villin expression in human tumors: a tissue microarray study on 14,398 tumors.  |  Dum, D., et al. 2022. Expert Rev Mol Diagn. 22: 665-675. PMID: 35866621
  9. Villin-1 regulates ferroptosis in colorectal cancer progression.  |  Hu, B., et al. 2024. FEBS J.. PMID: 39658274
  10. 人类球蛋白的完整一级结构及其与肌肉结构的相关性。  |  Labeit, S. and Kolmerer, B. 1995. J Mol Biol. 248: 308-15. PMID: 7739042
  11. Caldesmon 与 Ca(2+)-calmodulin 的多位相互作用。  |  Huber, PA., et al. 1996. Biochem J. 316 (Pt 2): 413-20. PMID: 8687382
  12. 克隆小鼠球蛋白的 cDNA。证明神经球蛋白编码序列在脊椎动物中高度保守。  |  Zhang, JQ., et al. 1996. Eur J Biochem. 239: 835-41. PMID: 8774733

订购信息

产品名称产品编号规格价格数量收藏夹

Villin 抗体 (1D2C3)

sc-58897
200 µg/ml
RMB2377.00

Villin (1D2C3): m-IgG Fc BP-HRP 套装

sc-528631
200 µg Ab; 10 µg BP
RMB2662.00

Villin (1D2C3): m-IgGκ BP-HRP 套装

sc-521061
200 µg Ab, 40 µg BP
RMB2662.00

Villin (1D2C3): m-IgG1 BP-HRP 套装

sc-543041
200 µg Ab; 20 µg BP
RMB2662.00

Villin 抗体 (1D2C3) AC

sc-58897 AC
500 µg/ml, 25% agarose
RMB3129.00

Villin 抗体 (1D2C3) HRP

sc-58897 HRP
200 µg/ml
RMB2377.00

Villin 抗体 (1D2C3) FITC

sc-58897 FITC
200 µg/ml
RMB2482.00

Villin 抗体 (1D2C3) PE

sc-58897 PE
200 µg/ml
RMB2580.00

Villin 抗体 (1D2C3) Alexa Fluor® 488

sc-58897 AF488
200 µg/ml
RMB2685.00

Villin 抗体 (1D2C3) Alexa Fluor® 546

sc-58897 AF546
200 µg/ml
RMB2685.00

Villin 抗体 (1D2C3) Alexa Fluor® 594

sc-58897 AF594
200 µg/ml
RMB2685.00

Villin 抗体 (1D2C3) Alexa Fluor® 647

sc-58897 AF647
200 µg/ml
RMB2685.00

Villin 抗体 (1D2C3) Alexa Fluor® 680

sc-58897 AF680
200 µg/ml
RMB2685.00

Villin 抗体 (1D2C3) Alexa Fluor® 790

sc-58897 AF790
200 µg/ml
RMB2685.00

Has your villin antibody (Villin Antibody (1D2C3): sc-58897) been tested against fish tissues (zebrafish)?

Asked by: TomFinger
Thank you for your question. We only recommend the antibody for mouse,rat,human and avian. There is a 64%homology in zebra fish and we only recommend a species if the homology is 95% or higher .
Answered by: BlakeJ
Date published: 2023-11-09

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-01-27
  • y_2025, m_9, d_6, h_5CST
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Rated 4 out of 5 by from works with IFThis antibody worked well for IF of mouse kidneys.
Date published: 2022-03-18
Rated 5 out of 5 by from Very good signalStrong and clear bands (Colorectal cancer stem-like cells).
Date published: 2021-07-06
Rated 5 out of 5 by from Excellent labelling of intestinal porcine villiI used this antibody to label intestinal villi on frozen porcine section 15um at 1:800 with triton perm
Date published: 2018-02-02
Rated 5 out of 5 by from 很好，条带很漂亮。两次就跑出来了。条带蛮清晰的，第一次没跑出来，后来改了下条件，就跑出来了。还是挺满意的。若果后面再用，还是会考录用这个抗体。
Date published: 2017-10-12
Rated 5 out of 5 by from Great for IFStrong, clean signal by IF in rat and mouse kidney.
Date published: 2017-03-22
Rated 5 out of 5 by from Highly recommended for IHC(P) and WB.strong distinct WB band in COLO 205 whole cell lysate, human colon tissue extract and human small intestine tissue extract.
Date published: 2017-01-11
Rated 5 out of 5 by from IHC publishable data with mouse Brush BorderIHC publishable data with mouse Brush Border Membrane Vesicles (BBMVs) - SCBT Publication Review
Date published: 2015-03-16
Rated 5 out of 5 by from Great immunoperoxidase apical membrane andGreat immunoperoxidase apical membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2014-01-24
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Villin 抗体 (1D2C3) is rated 4.9 out of 5 by 8.
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