Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Flk-1/KDR/VEGFR2 Antibody (D-8) - Immunohistochemistry - Image 353882
VEGFR2 抗体 (D-8): sc-393163. 福尔马林固定, 石蜡包埋的人类胎盘组织的免疫过氧化物酶染色显示滋养层细胞的胞浆染色以及内皮细胞的膜和胞浆染色.
VEGFR2 抗体 (D-8): sc-393163. 福尔马林固定, 石蜡包埋的人类胎盘组织的免疫过氧化物酶染色显示滋养层细胞的胞浆染色以及内皮细胞的膜和胞浆染色.

Flk-1/KDR/VEGFR2 抗体 (D-8): sc-393163

4.1(13)
寫評論提問

说明书
  • Flk-1/KDR/VEGFR2抗体(D-8)是小鼠单克隆IgG1 λ Flk-1/KDR/VEGFR2抗体, 在32篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Flk-1的C-terminus的氨基酸1282-1321之间
  • Flk-1/KDR/VEGFR2 抗体 (D-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 VEGFR2; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Flk-1/KDR/VEGFR2抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Flk-1/KDR/VEGFR2 (D-8): sc-393163的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 Flk-1/KDR/VEGFR2 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Flk-1/KDR/VEGFR2 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品，请参阅我们的 小鼠 IgG 结合蛋白完整列表。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 VEGFR / Flk 抗体 包括 Flt-3/Flk-2

Flk-1抗体（D-8）是一种IgG1 λ小鼠单克隆Flk-1抗体（也称为CD309抗体、KDR激酶插入域受体抗体或FLK1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Flk-1蛋白。Flk-1抗体（D-8）既有非偶联的抗Flk-1抗体形式，也有多种偶联形式的抗Flk-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。三种细胞膜受体酪氨酸激酶Flt（也称为VEGF-R1）、Flk-1（也称为VEGF-R2）和Flt-4，可能参与内皮细胞的生长，其特征是细胞外结构域中存在七个免疫球蛋白样序列。这些受体彼此之间具有高度序列相关性，与III类受体（包括CSF-1/Fms、PDGR、SLFR/Kit和Flt-3/Flk-2）具有较低程度的相关性。已显示该受体类的两个成员Flt-1和Flk-1代表血管内皮生长因子(VEGFs)的高亲和力受体。基于与Flt和Flk-1的结构相似性，推测Flt-4可能代表VEGF或VEGF相关配体的第三种受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Flk-1/KDR/VEGFR2 抗体 (D-8)

sc-393163
200 µg/ml
RMB2377.00

Flk-1/KDR/VEGFR2 (D-8): m-IgGλ BP-HRP 套装

sc-523832
200 µg Ab, 40 µg BP
RMB2662.00

Flk-1/KDR/VEGFR2 抗体 (D-8) AC

sc-393163 AC
500 µg/ml, 25% agarose
RMB3129.00

Flk-1/KDR/VEGFR2 抗体 (D-8) HRP

sc-393163 HRP
200 µg/ml
RMB2377.00

Flk-1/KDR/VEGFR2 抗体 (D-8) FITC

sc-393163 FITC
200 µg/ml
RMB2482.00

Flk-1/KDR/VEGFR2 抗体 (D-8) PE

sc-393163 PE
200 µg/ml
RMB2580.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 488

sc-393163 AF488
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 546

sc-393163 AF546
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 594

sc-393163 AF594
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 647

sc-393163 AF647
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 680

sc-393163 AF680
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 抗体 (D-8) Alexa Fluor® 790

sc-393163 AF790
200 µg/ml
RMB2685.00

Flk-1/KDR/VEGFR2 (D-8) 中和勝肽

sc-393163 P
100 µg/0.5 ml
RMB511.00

There is no signal using Flk-1(D8) on canine tissue - what kind of pretreatment would you suggest (Citrate? EDTA?); we already tried pH6, dil. 1:50-1:200

Asked by: Angelika2406
Thank you for your question. You can find pre-treatment options in our recommended protocol for IHC by following this link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining We recommend heat treatment with 10 mM sodium citrate buffer, Pepsin, or Saponin. Please do not hesitate to get in touch with our technical service if these options do not improve your results.
Answered by: Tech Support Europe
Date published: 2019-07-09

We would like to get free sample of Flk-1 Antibody (D-8): sc-393163 antibody. Is it possible?

Asked by: martakiezun
Thank you for your question. Please get in touch with our technical service to arrange for a free sample of this antibody.
Answered by: Technical Support Europe
Date published: 2019-05-06

Is Flk-1 Antibody (D-8): sc-393163 applicable for flow cytometry analysis? 

Asked by: CLab
Thanks for your question. This antibody has not been tested for Flow cytometry and is therefore not recommended for that use. It may work fine, but has not been tested. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-11-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-02-09
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_393163, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:250 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-04-01
Rated 3 out of 5 by from Not always convincingTested with different protocols on canine tissue (hemangiosarcoma), the results were not always convincing.
Date published: 2022-06-08
Rated 1 out of 5 by from Did not work for 1:500 dilutionTried 1:1000 and 1:500 dilution in human bladder cancer cell lines - no signal detected by WB analysis
Date published: 2018-04-03
Rated 4 out of 5 by from Good antibodyI detected great bands in mouse endothelial cells. Though, there was a bit of background, but nothing to affect my experiment.
Date published: 2017-10-24
Rated 5 out of 5 by from Effect is Quite GoodWhen we used Flk-1 Antibody in our aim cell, the results show is very good. The antibody has very good activity and improves the detecting sensitivity.
Date published: 2017-10-21
Rated 5 out of 5 by from Good for WB1:300 diluted for wb of rat protein ,the band is strong and special!
Date published: 2017-08-11
Rated 5 out of 5 by from Excellent antibody for Western blot.We obtained great results with this excellent antibody (working dilution 1:1000, blocking buffer: 5%no-fat dry milk) on rat brain and retina. It clearly detects three strong bands between 150 and 230 kDa, corresponding to immature, intermidiate glycosylated and mature glycosylated forms of Fkl1 (VEGFR2).
Date published: 2017-07-14
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_393163, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
Flk-1/KDR/VEGFR2 抗体 (D-8) is rated 4.1 out of 5 by 13.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_393163, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 112ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。